##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545155_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1615209
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.849508020324304	31.0	31.0	33.0	30.0	33.0
2	31.15416952233426	31.0	31.0	33.0	30.0	34.0
3	31.316648186086134	31.0	31.0	34.0	28.0	34.0
4	35.209999448987716	37.0	35.0	37.0	33.0	37.0
5	35.07128303519854	37.0	35.0	37.0	32.0	37.0
6	35.14402841985155	37.0	35.0	37.0	32.0	37.0
7	35.536917513461106	36.0	35.0	37.0	35.0	37.0
8	35.603300873137776	37.0	35.0	37.0	35.0	37.0
9	37.45659663857742	39.0	37.0	39.0	35.0	39.0
10	36.89150568130812	39.0	37.0	39.0	32.0	39.0
11	36.47445810418342	38.0	35.0	39.0	32.0	39.0
12	35.294365001680895	35.0	35.0	39.0	31.0	39.0
13	34.883138962202416	35.0	34.0	39.0	30.0	39.0
14	35.57475038833984	36.0	34.0	40.0	29.0	41.0
15	35.980198228216906	36.0	34.0	40.0	31.0	41.0
16	36.15851508999764	36.0	34.0	40.0	32.0	41.0
17	35.98994557360688	36.0	34.0	40.0	31.0	41.0
18	36.070894850140135	36.0	35.0	40.0	31.0	41.0
19	35.951551780605485	36.0	35.0	40.0	31.0	41.0
20	35.69673893595194	36.0	34.0	40.0	30.0	41.0
21	35.570734189816925	35.0	34.0	40.0	30.0	41.0
22	35.405804450074264	35.0	34.0	40.0	30.0	41.0
23	35.33717741790691	35.0	34.0	40.0	30.0	41.0
24	35.45475600990336	35.0	34.0	40.0	30.0	41.0
25	35.36503139841346	35.0	34.0	40.0	30.0	41.0
26	35.09865596340783	35.0	34.0	39.0	29.0	41.0
27	35.0914141761221	35.0	34.0	40.0	29.0	41.0
28	35.162204395839794	36.0	34.0	40.0	29.0	41.0
29	35.150899357296794	36.0	34.0	40.0	29.0	41.0
30	35.1372707804377	36.0	34.0	40.0	29.0	41.0
31	34.933717556056216	35.0	34.0	40.0	29.0	41.0
32	34.81125662375581	35.0	34.0	40.0	29.0	41.0
33	34.56535470022765	35.0	33.0	40.0	27.0	41.0
34	34.3682600827509	35.0	34.0	40.0	26.0	41.0
35	34.159405377260775	35.0	33.0	40.0	25.0	41.0
36	33.91435102206587	35.0	33.0	40.0	23.0	41.0
37	33.958154641287905	35.0	33.0	40.0	24.0	41.0
38	33.99311977583087	35.0	33.0	40.0	24.0	41.0
39	34.00778289373078	35.0	33.0	40.0	24.0	41.0
40	33.72520707846477	35.0	33.0	40.0	23.0	41.0
41	33.776402929899476	35.0	33.0	40.0	23.0	41.0
42	33.76211809121916	35.0	33.0	40.0	23.0	41.0
43	33.724501287449485	35.0	33.0	40.0	23.0	41.0
44	33.70365816436139	35.0	33.0	39.0	23.0	41.0
45	33.77052567190995	35.0	33.0	39.0	23.0	41.0
46	33.77180228688671	35.0	33.0	39.0	23.0	41.0
47	33.68826449084917	35.0	33.0	39.0	23.0	41.0
48	33.745719594182546	35.0	33.0	39.0	23.0	41.0
49	33.791373747917454	35.0	33.0	39.0	24.0	41.0
50	33.589731112196624	35.0	33.0	39.0	24.0	41.0
51	33.301373382639646	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	0.0
14	10.0
15	42.0
16	108.0
17	334.0
18	838.0
19	1875.0
20	3277.0
21	5486.0
22	8326.0
23	12018.0
24	16906.0
25	23500.0
26	30156.0
27	34491.0
28	38217.0
29	44054.0
30	52437.0
31	64583.0
32	81317.0
33	108242.0
34	190288.0
35	273949.0
36	96643.0
37	119820.0
38	172181.0
39	236036.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.31228899789439	24.134833324975283	30.526018614309358	13.026859062820972
2	29.66718238939976	26.358384580571308	32.17558842230324	11.798844607725687
3	26.611602585176286	27.218520946824835	34.05584045160719	12.114036016391687
4	25.110125067406138	28.758445501479997	32.793093649181	13.338335781932864
5	21.87190636010572	34.17724888853393	31.59386803813005	12.3569767132303
6	19.613994226134203	42.21688957899566	28.86796693183359	9.301149263036548
7	87.82646703925003	4.079162510857728	6.866417906289526	1.2279525436027163
8	89.95869884330759	2.7626765328821223	6.341779918264448	0.9368447055458458
9	86.06192758955652	4.739015198652311	7.513145357659597	1.6859118541315707
10	56.01504201623443	24.72330206183844	12.87845721513439	6.383198706792744
11	50.202791093907976	17.59270781675932	22.655024829604095	9.549476259728618
12	47.500416354787525	21.147665720039946	22.40094006410316	8.950977861069372
13	18.489867255568786	49.97836193334733	20.844485140932225	10.687285670151665
14	10.731366652860403	50.937742422188094	25.697046016955085	12.633844907996428
15	8.980200085561684	25.150491360560768	54.73526955335192	11.134039000525627
16	10.474062489745908	18.162726928837074	53.49611102959432	17.867099551822704
17	10.873948820245554	19.189714767562588	31.365228896074747	38.57110751611711
18	15.702673771629556	22.53547373745441	44.47189187281646	17.289960618099578
19	30.634859018244697	21.53269329232316	28.802836041651574	19.029611647780566
20	31.733911834319894	21.041116041329637	31.320962178888305	15.904009945462166
21	16.663230578829115	33.750554881752144	31.813034721822376	17.77317981759636
22	19.579571436266143	24.103506109735644	24.143562845427432	32.17335960857078
23	12.706776646242066	33.945080791402226	27.202238224279334	26.14590433807637
24	10.424533295691147	21.871225333687466	54.986073009746725	12.71816836087466
25	10.440939841221786	26.688434747453737	47.264471656609146	15.606153754715335
26	11.33258915719266	40.80889841500388	29.133691057937394	18.724821369866067
27	10.278545996214731	48.75313349541762	27.081510813770848	13.8868096945968
28	9.157081219829756	35.11570329288656	42.58006239440221	13.147153092881478
29	9.335014849471493	25.175008311617873	41.755339401897835	23.734637437012797
30	13.55038264397982	42.35210427876516	30.94633573735659	13.151177339898428
31	29.611647780565857	31.744808257011943	26.817396386473824	11.826147575948376
32	32.73749712885453	23.740642851791936	30.37018738751456	13.151672631838975
33	32.945457832391966	28.508570717473713	22.729070974715963	15.816900475418352
34	18.290636072483498	26.515454037217474	26.219083722292286	28.97482616800674
35	17.82208989672544	24.862479097132322	35.757973116791696	21.55745788935054
36	34.794073088993436	27.915520530160492	24.116383700189882	13.174022680656188
37	15.623303238156797	44.07739184217027	28.818190091808553	11.481114827864381
38	15.172401837780743	41.591397769576574	25.837708928070608	17.39849146457208
39	18.004481153832106	40.04868719775583	28.610972326181937	13.335859322230126
40	26.605535258904577	25.43807024354124	26.635190863844866	21.32120363370932
41	13.448785884674985	24.934853631944843	29.738690163316328	31.877670320063846
42	15.349840175481935	22.325841423617625	28.111965696080198	34.21235270482024
43	16.228488078013434	22.233655211183198	30.949307488999878	30.588549221803497
44	12.274572516621687	29.992836840309828	35.81654138876145	21.916049254307026
45	11.033123267639048	51.759617486034315	22.318102487046566	14.889156759280068
46	17.597536913179656	43.06557231912403	24.38439855151872	14.952492216177596
47	16.57333509161972	30.850187189397783	26.32662398488369	26.249853734098807
48	16.713688445272407	28.300238544980864	39.476439271945615	15.509633737801115
49	21.080429839110604	27.603486607615483	38.25529699252543	13.060786560748486
50	18.49599649333306	42.4849044303245	25.945434925139722	13.073664151202724
51	15.294924681573715	38.21753098205867	26.277899640232317	20.2096446961353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1707.0
1	2204.5
2	2702.0
3	7457.0
4	12212.0
5	9768.5
6	7325.0
7	7656.0
8	7987.0
9	8389.0
10	8791.0
11	8954.0
12	9117.0
13	8955.5
14	8794.0
15	8100.0
16	7406.0
17	6956.0
18	6506.0
19	6428.0
20	6350.0
21	6655.5
22	6961.0
23	9684.0
24	12407.0
25	13011.5
26	19089.5
27	24563.0
28	30421.0
29	36279.0
30	44047.0
31	51815.0
32	57258.0
33	62701.0
34	68734.0
35	74767.0
36	78867.5
37	82968.0
38	94763.5
39	106559.0
40	146244.5
41	185930.0
42	213400.0
43	240870.0
44	227818.5
45	214767.0
46	182620.5
47	150474.0
48	127784.0
49	105094.0
50	87540.0
51	69986.0
52	52492.0
53	34998.0
54	26833.0
55	18668.0
56	15027.5
57	11387.0
58	9986.0
59	8585.0
60	7748.5
61	6912.0
62	5907.0
63	4902.0
64	4166.0
65	3430.0
66	2916.0
67	2402.0
68	2127.0
69	1852.0
70	1609.5
71	1367.0
72	1164.5
73	962.0
74	769.5
75	421.5
76	266.0
77	198.5
78	131.0
79	95.0
80	59.0
81	43.0
82	27.0
83	20.5
84	14.0
85	10.0
86	6.0
87	4.5
88	3.0
89	5.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1615209.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.10426041615239
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89266779795389	13.665049953573554
2	7.5479569480141135	2.5820444249748036
3	2.6523690968315297	1.3610043525588418
4	1.461954198460886	1.000225813078493
5	0.9517594295191211	0.8139570568011839
6	0.6774496561335466	0.6952365202404642
7	0.5426441388632112	0.6497068665090586
8	0.42742841612019766	0.5848677550866728
9	0.3606986358643382	0.5552535059617107
>10	3.422685802217809	12.610188485216417
>50	0.733139291802875	9.008267897127077
>100	1.28272114023048	50.501905284824986
>500	0.042163687239086325	4.722103962521294
>1k	0.003998280686465083	0.8656009357003447
>5k	3.634800624059166E-4	0.3845871858250615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6186	0.3829844930284564	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1906	0.1180033048354733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.020616527025295178	0.0
2	0.0	0.0	0.0	0.07745127720313594	0.0
3	0.0	0.0	0.0	0.10822128900965758	0.0
4	0.0	0.0	0.0	0.16728485291996267	0.0
5	0.0	0.0	0.0	0.3065237997064157	0.0
6	0.0	0.0	0.0	0.4081205590112487	0.0
7	0.0	0.0	0.0	0.4795664214352446	0.0
8	0.0	0.0	0.0	0.6514327248052729	0.0
9	0.0	0.0	0.0	0.7065339531911969	0.0
10	0.0	0.0	0.0	0.8403866001241944	0.0
11	6.191149256845399E-5	0.0	0.0	0.9538084545096022	0.0
12	6.191149256845399E-5	0.0	0.0	1.0656825215807986	0.0
13	6.191149256845399E-5	0.0	0.0	1.1082776284678948	0.0
14	6.191149256845399E-5	0.0	0.0	1.1238793245951453	0.0
15	6.191149256845399E-5	0.0	0.0	1.153844486998277	0.0
16	6.191149256845399E-5	0.0	0.0	1.2199659610613858	0.0
17	6.191149256845399E-5	0.0	0.0	1.3002032554301022	0.0
18	6.191149256845399E-5	0.0	0.0	1.4135012868303731	0.0
19	6.191149256845399E-5	0.0	0.0	1.470212214023077	0.0
20	6.191149256845399E-5	0.0	0.0	1.5248800619610217	0.0
21	6.191149256845399E-5	0.0	0.0	1.611246594094015	0.0
22	6.191149256845399E-5	0.0	0.0	1.7036185410061484	0.0
23	6.191149256845399E-5	0.0	0.0	1.8123351219563537	0.0
24	6.191149256845399E-5	0.0	0.0	1.8985159196116417	0.0
25	6.191149256845399E-5	0.0	0.0	1.9689711981545421	0.0
26	6.191149256845399E-5	0.0	0.0	2.0457414489394252	0.0
27	6.191149256845399E-5	0.0	0.0	2.1180540722593793	0.0
28	6.191149256845399E-5	0.0	0.0	2.1950100575219675	0.0
29	6.191149256845399E-5	0.0	0.0	2.285896128612458	0.0
30	6.191149256845399E-5	0.0	0.0	2.406995008076354	0.0
31	6.191149256845399E-5	0.0	0.0	2.522707587686795	0.0
32	6.191149256845399E-5	0.0	0.0	2.6263474262463866	0.0
33	6.191149256845399E-5	0.0	0.0	2.728129920028925	0.0
34	6.191149256845399E-5	0.0	0.0	2.832760342469612	0.0
35	6.191149256845399E-5	0.0	0.0	2.9916252324002652	0.0
36	6.191149256845399E-5	0.0	0.0	3.1148291026114885	0.0
37	6.191149256845399E-5	0.0	0.0	3.238156795807849	0.0
38	6.191149256845399E-5	0.0	0.0	3.3544884903439742	0.0
39	6.191149256845399E-5	0.0	0.0	3.479116324884272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	80	0.0	45.000004	1
AATCCGG	20	7.0341356E-4	45.000004	2
TAGACGC	20	7.0341356E-4	45.000004	30
CTAAGCG	60	0.0	45.000004	1
GCGTTAG	155	0.0	45.000004	1
ATCCGGA	20	7.0341356E-4	45.000004	12
AATGCGT	20	7.0341356E-4	45.000004	16
CTACGCG	30	2.1660508E-6	45.000004	1
TGCGAAG	80	0.0	45.000004	1
ACGGACC	30	2.1660508E-6	45.000004	39
GCCTCGT	40	6.8175723E-9	45.000004	13
TTACGCG	75	0.0	45.000004	1
CGATGCG	30	2.1660508E-6	45.000004	10
GCGTCAG	20	7.0341356E-4	45.000004	19
TGTCGTT	20	7.0341356E-4	45.000004	31
TATCGCT	40	6.8175723E-9	45.000004	44
ACCTTCG	40	6.8175723E-9	45.000004	23
TCCGGCA	50	2.1827873E-11	45.0	10
GATCGCG	25	3.891511E-5	45.0	1
ATTACCG	35	1.2123382E-7	45.0	1
>>END_MODULE