FastQCFastQC Report
Thu 26 May 2016
SRR1545154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545154_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1690026
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.29644514344749723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.034229E-445.0000041
AACCGTG207.034229E-445.00000424
AACCGGT207.034229E-445.00000419
CGGCGAA207.034229E-445.00000431
GTACCGT207.034229E-445.00000418
ACACGTC406.8175723E-945.00000422
CTCGTGA207.034229E-445.00000436
CACGGGT207.034229E-445.00000440
GCGAATA207.034229E-445.0000041
GCGCGAC207.034229E-445.0000049
CGCATTG207.034229E-445.00000412
CCGGACC207.034229E-445.00000417
CAACCGG207.034229E-445.0000042
CTAAGCG650.045.0000041
TGACGTC207.034229E-445.00000432
CGACAAG207.034229E-445.00000427
GCGTTAT207.034229E-445.00000423
CCGTCGC207.034229E-445.00000421
GCACCGC207.034229E-445.0000043
CGTTAGA800.045.00000421