##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545154_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1690026
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.881836137432206	31.0	31.0	33.0	30.0	33.0
2	31.20376254566498	31.0	31.0	33.0	30.0	34.0
3	31.367719194852622	31.0	31.0	34.0	30.0	34.0
4	35.241138893721164	37.0	35.0	37.0	33.0	37.0
5	35.140449318531196	37.0	35.0	37.0	32.0	37.0
6	35.20495069306626	37.0	35.0	37.0	32.0	37.0
7	35.54462357383851	36.0	35.0	37.0	35.0	37.0
8	35.60372029779423	37.0	35.0	37.0	35.0	37.0
9	37.464594035831404	39.0	37.0	39.0	35.0	39.0
10	36.955527311414144	39.0	37.0	39.0	33.0	39.0
11	36.55153944377187	39.0	35.0	39.0	32.0	39.0
12	35.461573372243976	37.0	35.0	39.0	31.0	39.0
13	35.15150358633536	37.0	35.0	39.0	30.0	39.0
14	35.84804020766544	37.0	35.0	40.0	30.0	41.0
15	36.19953598347008	37.0	35.0	40.0	31.0	41.0
16	36.31643300162246	37.0	35.0	40.0	32.0	41.0
17	36.14008423539046	36.0	34.0	40.0	31.0	41.0
18	36.2138789580752	36.0	35.0	40.0	31.0	41.0
19	36.14920953878816	36.0	35.0	40.0	31.0	41.0
20	35.93707789110937	36.0	34.0	40.0	31.0	41.0
21	35.80730769822476	36.0	34.0	40.0	31.0	41.0
22	35.63329380731421	35.0	34.0	40.0	30.0	41.0
23	35.57317283876106	35.0	34.0	40.0	30.0	41.0
24	35.669239408151114	35.0	34.0	40.0	30.0	41.0
25	35.57042495204216	35.0	34.0	40.0	30.0	41.0
26	35.34492546268519	35.0	34.0	40.0	30.0	41.0
27	35.335177091950065	35.0	34.0	40.0	30.0	41.0
28	35.42006454338572	36.0	34.0	40.0	30.0	41.0
29	35.39339394778542	36.0	34.0	40.0	30.0	41.0
30	35.376732073944424	36.0	34.0	40.0	30.0	41.0
31	35.17824696188106	36.0	34.0	40.0	29.0	41.0
32	35.03787752377774	35.0	34.0	40.0	29.0	41.0
33	34.823060710308596	35.0	34.0	40.0	27.0	41.0
34	34.65501477492062	35.0	34.0	40.0	27.0	41.0
35	34.446562360579065	35.0	33.0	40.0	26.0	41.0
36	34.235195198180385	35.0	33.0	40.0	24.0	41.0
37	34.26443261819641	35.0	33.0	40.0	25.0	41.0
38	34.3180826803848	35.0	33.0	40.0	25.0	41.0
39	34.33381202419371	35.0	33.0	40.0	25.0	41.0
40	33.978379030855145	35.0	33.0	40.0	23.0	41.0
41	34.07977806258602	35.0	33.0	40.0	23.0	41.0
42	34.08600518571904	35.0	33.0	40.0	24.0	41.0
43	34.06663506951964	35.0	33.0	40.0	24.0	41.0
44	34.03466278033592	35.0	33.0	40.0	24.0	41.0
45	34.11845084040127	35.0	33.0	40.0	24.0	41.0
46	34.12675662977966	35.0	33.0	40.0	24.0	41.0
47	34.05885530755148	35.0	33.0	40.0	24.0	41.0
48	34.09225124347199	35.0	33.0	40.0	24.0	41.0
49	34.149477582001694	36.0	33.0	40.0	24.0	41.0
50	33.955360450075915	35.0	33.0	40.0	24.0	41.0
51	33.684836801327315	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	1.0
14	11.0
15	48.0
16	118.0
17	338.0
18	784.0
19	1581.0
20	2795.0
21	4821.0
22	7661.0
23	11133.0
24	16338.0
25	22548.0
26	28731.0
27	32848.0
28	36474.0
29	42425.0
30	51804.0
31	64498.0
32	82755.0
33	109460.0
34	194738.0
35	280242.0
36	106081.0
37	132403.0
38	193953.0
39	265352.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.53719173551176	25.522270071584696	28.96718748705641	12.973350705847128
2	31.435966073894722	25.450377686497134	30.7637870659978	12.349869173610346
3	27.367922150310115	25.975280853667336	33.816225312509985	12.840571683512561
4	25.216949325039973	29.70143654594663	31.976194449079482	13.105419679933918
5	22.63746238223554	33.33741611075806	31.362121056125762	12.663000450880638
6	19.445736337784155	41.4501315364379	29.685460460371615	9.41867166540633
7	89.05922157410596	3.753255867069501	6.223277038341422	0.9642455204831168
8	91.05842158641346	2.530079418896514	5.56322802134405	0.8482709733459721
9	87.282621687477	4.203840650972233	6.837527943357085	1.6760097181936846
10	54.619573900046504	28.060100850519458	11.046220590689138	6.2741046587448945
11	47.69784606864037	19.1900597978966	23.750344669253607	9.361749464209426
12	44.60700604606083	23.537626048356653	22.48509786239975	9.370270043182767
13	18.021675406177184	48.845698231861526	22.755507903428704	10.37711845853259
14	11.765676977750639	49.36184413730913	27.40827655905885	11.464202325881377
15	10.613031988856976	24.998787000910045	53.90769136096131	10.480489649271668
16	11.63047195723616	20.310042567392454	51.67322869588988	16.3862567794815
17	12.606788297931512	19.20183476467226	31.322772549061373	36.86860438833485
18	16.312293420337912	24.055014538237874	43.53217051098623	16.100521530437994
19	30.268055047673826	22.067944516829918	30.275865578399387	17.388134857096873
20	31.609336187727287	22.681781227034378	29.349252615048528	16.359629970189808
21	17.15452898357777	34.35994475824632	32.35382177552298	16.13170448265293
22	19.403606808415965	24.532166960744984	24.816659625354877	31.247566605484177
23	13.564170018686102	34.369767092340595	27.90377189463357	24.162290994339735
24	11.395505157908813	22.16652288189649	53.154389340755706	13.283582619438992
25	11.069474670803881	26.872959350921228	45.67580617102932	16.38175980724557
26	11.461362132890262	41.01398440023999	30.17302692384614	17.3516265430236
27	10.704214018009191	47.74399920474596	28.056905633404455	13.49488114384039
28	10.246706263690617	34.606864036411274	42.030418466934826	13.11601123296328
29	10.638061189591166	26.40758189518978	41.11291778943046	21.841439125788597
30	14.508652529606053	42.058583714096706	30.239771459137316	13.192992297159925
31	29.924746719872946	30.849111197105845	27.372359951858726	11.853782131162479
32	32.316840095951186	24.31731819510469	30.16776073267512	13.198080976269006
33	33.24061286631093	28.80494146243904	22.924736069149233	15.029709602100796
34	18.664209899729354	27.872293088982065	25.775875637416227	27.687621373872357
35	17.815879755696066	27.722946274199334	35.6372032146251	18.8239707554795
36	34.38071366949384	27.242953658701108	24.79873090709847	13.577601764706579
37	16.07241545396343	43.46678690150329	28.06749718643382	12.39330045809946
38	16.518917460441436	41.62255491927343	25.002041388712364	16.856486231572767
39	18.853260245700362	39.852996344434935	26.93999973964897	14.353743670215724
40	26.01208502117719	25.683746877266977	27.62484127463128	20.67932682692456
41	13.386302932617605	26.178768847343175	28.582637190197076	31.852291029842146
42	16.335014964266822	22.71148491206644	27.40531802469311	33.548182098973626
43	16.832758785959506	23.70602582445477	30.120720036259797	29.340495353325924
44	12.523949335690693	31.836610797703703	35.52625817590972	20.113181690695882
45	11.727216030995972	50.029111978158916	23.48656174520392	14.757110245641192
46	16.61033617234291	41.89923705315776	25.91090314586876	15.579523628630565
47	17.226953904851168	30.814496345026644	26.125988594258313	25.832561155863875
48	17.36162638917981	28.314949000784605	38.36917301863995	15.954251591395636
49	20.979854747796782	28.11980407402016	37.6177052897411	13.282635888441954
50	17.873926199951953	43.89257916742109	25.060087832968247	13.173406799658704
51	16.708500342598278	38.965909400210414	25.3981299695981	18.92746028759321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1436.0
1	1901.0
2	2366.0
3	6367.0
4	10368.0
5	8501.0
6	6634.0
7	7073.0
8	7512.0
9	7973.0
10	8434.0
11	8477.5
12	8521.0
13	8479.5
14	8438.0
15	7963.5
16	7489.0
17	7053.5
18	6618.0
19	6972.0
20	7326.0
21	7734.0
22	8142.0
23	9557.5
24	10973.0
25	13258.5
26	20062.0
27	24580.0
28	29924.0
29	35268.0
30	42666.5
31	50065.0
32	57315.0
33	64565.0
34	75951.5
35	87338.0
36	92442.5
37	97547.0
38	112230.0
39	126913.0
40	156133.5
41	185354.0
42	219620.5
43	253887.0
44	243051.0
45	232215.0
46	193348.5
47	154482.0
48	132621.5
49	110761.0
50	91480.5
51	72200.0
52	54865.5
53	37531.0
54	27568.0
55	17605.0
56	13787.0
57	9969.0
58	8256.0
59	6543.0
60	5679.5
61	4816.0
62	3926.5
63	3037.0
64	2635.0
65	2233.0
66	1748.0
67	1263.0
68	1004.5
69	746.0
70	584.5
71	423.0
72	401.0
73	379.0
74	322.0
75	191.0
76	117.0
77	83.5
78	50.0
79	46.0
80	42.0
81	29.5
82	17.0
83	11.5
84	6.0
85	6.0
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1690026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.25629988469531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57811867236661	12.93645761397863
2	7.369628768583665	2.3960579060194775
3	2.6947468215615697	1.314198373339032
4	1.4495760670017805	0.9425897300343249
5	0.9519721399566494	0.7737772294505214
6	0.6985387634894649	0.6813393372221396
7	0.5337138650999874	0.6073348850579651
8	0.4279103112950023	0.5564990675331903
9	0.35453376659724234	0.518706650614981
>10	3.7125168113416693	12.990514939251991
>50	0.7948309563446643	9.22087042675799
>100	1.3856528515072195	51.48006268636559
>500	0.044969736341791015	4.752780260689147
>1k	0.0029248609002790905	0.5310452015113426
>5k	3.656076125348863E-4	0.29776569217367943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5010	0.29644514344749723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02065056987288953	0.0
2	0.0	0.0	0.0	0.07195155577488158	0.0
3	0.0	0.0	0.0	0.10242445974203947	0.0
4	0.0	0.0	0.0	0.1564472972605155	0.0
5	0.0	0.0	0.0	0.2727176978342345	0.0
6	0.0	0.0	0.0	0.37869239881516614	0.0
7	0.0	0.0	0.0	0.4410583032450388	0.0
8	0.0	0.0	0.0	0.6072687639125078	0.0
9	0.0	0.0	0.0	0.6716464717110862	0.0
10	0.0	0.0	0.0	0.7951356961372192	0.0
11	0.0	0.0	0.0	0.9177373602536293	0.0
12	0.0	0.0	0.0	1.0302800075265115	0.0
13	0.0	0.0	0.0	1.078149093564241	0.0
14	0.0	0.0	0.0	1.0959594704460167	0.0
15	0.0	0.0	0.0	1.1274382760975274	0.0
16	0.0	0.0	0.0	1.1943603234506452	0.0
17	0.0	0.0	0.0	1.2841814267946174	0.0
18	0.0	0.0	0.0	1.4089724063416775	0.0
19	0.0	0.0	0.0	1.469208166028215	0.0
20	0.0	0.0	0.0	1.5270179275348426	0.0
21	0.0	0.0	0.0	1.604472357230007	0.0
22	0.0	0.0	0.0	1.689974000399994	0.0
23	0.0	0.0	0.0	1.792102606705459	0.0
24	0.0	0.0	0.0	1.867486062344603	0.0
25	0.0	0.0	0.0	1.9303845029603095	0.0
26	0.0	0.0	0.0	1.998253281310465	0.0
27	0.0	0.0	0.0	2.067778838905437	0.0
28	0.0	0.0	0.0	2.143576489355785	0.0
29	0.0	0.0	0.0	2.224166965478638	0.0
30	0.0	0.0	0.0	2.346709458907733	0.0
31	0.0	0.0	0.0	2.4509090392692183	0.0
32	0.0	0.0	0.0	2.5465880406573627	0.0
33	0.0	0.0	0.0	2.6442196747268976	0.0
34	0.0	0.0	0.0	2.7504902291444036	0.0
35	0.0	0.0	0.0	2.891671489077683	0.0
36	0.0	0.0	0.0	3.004746672536399	0.0
37	0.0	0.0	0.0	3.1193011231779866	0.0
38	0.0	0.0	0.0	3.2346839634419826	0.0
39	0.0	0.0	0.0	3.3587648947412645	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.034229E-4	45.000004	1
AACCGTG	20	7.034229E-4	45.000004	24
AACCGGT	20	7.034229E-4	45.000004	19
CGGCGAA	20	7.034229E-4	45.000004	31
GTACCGT	20	7.034229E-4	45.000004	18
ACACGTC	40	6.8175723E-9	45.000004	22
CTCGTGA	20	7.034229E-4	45.000004	36
CACGGGT	20	7.034229E-4	45.000004	40
GCGAATA	20	7.034229E-4	45.000004	1
GCGCGAC	20	7.034229E-4	45.000004	9
CGCATTG	20	7.034229E-4	45.000004	12
CCGGACC	20	7.034229E-4	45.000004	17
CAACCGG	20	7.034229E-4	45.000004	2
CTAAGCG	65	0.0	45.000004	1
TGACGTC	20	7.034229E-4	45.000004	32
CGACAAG	20	7.034229E-4	45.000004	27
GCGTTAT	20	7.034229E-4	45.000004	23
CCGTCGC	20	7.034229E-4	45.000004	21
GCACCGC	20	7.034229E-4	45.000004	3
CGTTAGA	80	0.0	45.000004	21
>>END_MODULE