FastQCFastQC Report
Thu 26 May 2016
SRR1545153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545153_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1807473
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.3441821814212439No Hit
TTTAACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT21390.11834201672722082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19510.10794075485498261No Hit
GTTGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA18190.10063774120000685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1150.045.00000425
ACTATCA302.1661945E-645.00000437
ACGCCTA302.1661945E-645.00000435
CAAGTCG750.045.0000041
GTTTACG1350.045.0000041
TTGTCGA750.045.00000426
ACGGGTA1500.045.0000045
ATCGTAG453.8562575E-1045.0000041
CACCTGG750.045.0000042
AGCGCAT302.1661945E-645.00000441
TATTACG600.045.0000041
TCCCGTA302.1661945E-645.00000430
GGTTGCG453.8562575E-1045.0000041
TACTAAG1150.045.0000041
TCGACAC302.1661945E-645.00000434
GTTATCG1150.045.0000041
TACCGGT600.045.00000419
CGGACAA207.0343615E-445.031
CAGCGCG800.045.01
CCTTACG207.0343615E-445.043
Produced by FastQC (version 0.11.5)