##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545153_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1807473 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.909052583358093 31.0 31.0 33.0 30.0 33.0 2 31.222973178575835 31.0 31.0 33.0 30.0 34.0 3 31.389412732583004 31.0 31.0 34.0 30.0 34.0 4 35.23784642979452 37.0 35.0 37.0 33.0 37.0 5 35.1163823747298 37.0 35.0 37.0 32.0 37.0 6 35.19784306598218 37.0 35.0 37.0 32.0 37.0 7 35.58373596728693 36.0 35.0 37.0 35.0 37.0 8 35.65052092064446 37.0 35.0 37.0 35.0 37.0 9 37.51763539483024 39.0 37.0 39.0 35.0 39.0 10 37.041242663099254 39.0 37.0 39.0 33.0 39.0 11 36.58445907629049 39.0 35.0 39.0 32.0 39.0 12 34.92048456602118 35.0 34.0 39.0 30.0 39.0 13 34.4144255543513 35.0 33.0 39.0 27.0 39.0 14 35.00304679516651 35.0 33.0 40.0 27.0 41.0 15 35.53389345235033 35.0 33.0 40.0 30.0 41.0 16 35.784173262892445 35.0 34.0 40.0 32.0 41.0 17 35.641871275532196 35.0 34.0 40.0 31.0 41.0 18 35.76470409239862 36.0 35.0 39.0 31.0 41.0 19 35.61363406258351 36.0 34.0 39.0 31.0 41.0 20 35.30704967653735 35.0 34.0 39.0 30.0 41.0 21 35.151825227818065 35.0 34.0 39.0 30.0 41.0 22 34.99498028462943 35.0 34.0 39.0 30.0 41.0 23 34.98639537077456 35.0 34.0 39.0 30.0 41.0 24 35.103799614157445 35.0 34.0 39.0 30.0 41.0 25 35.006957227023584 35.0 34.0 39.0 30.0 41.0 26 34.759482990893915 35.0 34.0 39.0 29.0 41.0 27 34.744075845116356 35.0 34.0 39.0 29.0 41.0 28 34.87111010786883 35.0 34.0 39.0 29.0 41.0 29 34.876617244075014 35.0 34.0 39.0 29.0 41.0 30 34.83841086422868 35.0 34.0 38.0 29.0 41.0 31 34.54354283577127 35.0 34.0 39.0 29.0 41.0 32 34.367128029021735 35.0 34.0 39.0 29.0 41.0 33 34.13611047025322 35.0 33.0 39.0 27.0 41.0 34 33.961203846475165 35.0 34.0 39.0 26.0 41.0 35 33.73479769822288 35.0 33.0 38.0 24.0 41.0 36 33.43980242028512 35.0 33.0 38.0 23.0 41.0 37 33.466988995132986 35.0 33.0 38.0 23.0 41.0 38 33.563786568319415 35.0 33.0 38.0 24.0 41.0 39 33.57360248258203 35.0 33.0 38.0 23.0 41.0 40 33.17534425133875 35.0 32.0 38.0 23.0 41.0 41 33.324596273360655 35.0 32.0 38.0 23.0 41.0 42 33.32697694516045 35.0 33.0 38.0 23.0 41.0 43 33.288961439534646 35.0 33.0 38.0 23.0 41.0 44 33.25904232041087 35.0 33.0 38.0 23.0 41.0 45 33.33727806722424 35.0 33.0 38.0 23.0 41.0 46 33.37277956572519 35.0 33.0 38.0 23.0 41.0 47 33.31109012416783 35.0 33.0 38.0 23.0 41.0 48 33.38256837031591 35.0 33.0 38.0 23.0 41.0 49 33.46396101075922 35.0 33.0 38.0 24.0 40.0 50 33.20980285735942 35.0 33.0 38.0 24.0 40.0 51 32.86531195763367 35.0 32.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 0.0 11 0.0 12 2.0 13 8.0 14 10.0 15 38.0 16 120.0 17 380.0 18 897.0 19 1948.0 20 3587.0 21 5865.0 22 9173.0 23 13637.0 24 19177.0 25 26737.0 26 34515.0 27 40363.0 28 44806.0 29 50700.0 30 61528.0 31 75846.0 32 97724.0 33 131325.0 34 249341.0 35 371559.0 36 91050.0 37 108377.0 38 154175.0 39 214474.0 40 109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.283454856587067 25.384556228502447 30.68554827651644 12.646440638394047 2 30.219317245679466 26.638406216856353 31.68003062839666 11.462245909067521 3 26.720177839447672 25.751366687081912 35.122627004663414 12.405828468807002 4 25.006293316691313 28.30648092668604 33.107493168639316 13.579732587983335 5 21.436115504906574 33.47137135658458 31.98116928994237 13.111343848566479 6 18.188321485300197 42.15825077331722 30.061195934877034 9.592231806505547 7 88.77349758474953 3.8081896659037233 6.242693528478711 1.1756192208680296 8 90.57192002314835 2.2629383675440797 5.935911629108706 1.2292299801988744 9 87.6425263337267 3.5684627100930415 7.224672235767837 1.5643387204124213 10 65.64037194469849 17.95434841903586 10.586271551497587 5.819008084768072 11 61.15842394326222 13.9318263675308 17.461837604213176 7.4479120849938 12 54.35306640818425 19.69185708444884 18.074128908149664 7.88094759921725 13 16.262760218271588 57.72141547895875 17.82151102672073 8.194313276048938 14 8.353430452349771 57.15825353961027 25.302341999022943 9.18597400901701 15 7.435574417985774 20.54332208558579 63.02583773035614 8.9952657660723 16 7.644208239901785 13.817246509353112 60.759801114594794 17.778744136150305 17 8.400900041107114 14.758007450180447 30.58142500607201 46.259667502640426 18 15.27076753013738 20.299169060893302 48.26130182857503 16.168761580394285 19 34.8894285004534 17.322471760297386 28.20528992687581 19.58280981237341 20 34.71266237448637 19.603612336117884 28.983448162157888 16.70027712723786 21 14.563979655574386 35.37590879642462 32.27301320683629 17.787098341164707 22 18.55922605759533 23.84765913515721 20.39067803502459 37.202436772222875 23 11.054107032304216 36.05918318005303 24.675057386749348 28.2116524008934 24 8.743643750141771 19.247092487688615 59.52083378285595 12.48842997931366 25 8.35968227464532 23.853081069537417 51.99513353726446 15.792103118552808 26 8.554539957166718 43.65171706575977 29.146327497008258 18.647415480065263 27 8.434593490469844 53.72943330273813 26.179312222091284 11.65666098470074 28 7.288186324221717 35.715720234825085 45.03348044479779 11.962612996155405 29 8.398742332527236 24.218508381591317 43.43860184910093 23.944147436780522 30 13.749527655461518 44.1571188062007 29.103505280576808 12.989848257760976 31 35.10514403258029 29.738701490976627 24.77807413997332 10.378080336469758 32 38.58558329778647 20.468521521483307 30.178597411966873 10.76729776876335 33 39.02409607225115 25.614047900023955 20.22232143993299 15.139534587791905 34 19.009025307708608 25.51313352951884 24.374748613118978 31.103092549653578 35 18.162373656480625 23.087426478846435 36.66217973933774 22.088020125335206 36 41.491795451439664 24.9328758991144 21.85122544015872 11.72410320928722 37 15.205980946879983 46.54537024896084 27.326825905559858 10.921822898599315 38 16.001456176662114 44.151862849403564 21.49520352447865 18.35147744945568 39 19.701151829100628 40.182564276202186 26.00470380470414 14.111580089993044 40 29.029589930250687 22.309600198730493 24.191122080385156 24.469687790633664 41 12.475649705417453 22.12945919524109 27.377670371839578 38.01722072750188 42 15.523662040871427 18.545394592339694 26.553702323630834 39.377241043158044 43 16.215235303653223 18.554357381825344 31.21413155272582 34.01627576179561 44 10.67573346877104 30.633210011989114 36.514570342129595 22.176486177110252 45 9.659618705231004 55.503457036426 20.60561900509717 14.231305253245829 46 17.88021176526565 42.52738491805964 24.466866171721513 15.125537144953203 47 17.46133967146397 28.396219473264605 24.45978446151063 29.68265639376079 48 16.973254925523094 25.96221354343882 42.17069909204729 14.89383243899079 49 22.416655739809116 25.355344173882543 39.91368059163263 12.314319494675715 50 18.823849650866155 45.79780721482423 22.835417181888747 12.542925952420866 51 16.416676763636303 39.89271209030508 23.351552139368057 20.339059006690558 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2680.0 1 3193.5 2 3707.0 3 7870.5 4 12034.0 5 9719.0 6 7404.0 7 7884.0 8 8364.0 9 9150.0 10 9936.0 11 10130.0 12 10324.0 13 9850.5 14 9377.0 15 8649.5 16 7922.0 17 7493.5 18 7065.0 19 6667.0 20 6269.0 21 6990.5 22 7712.0 23 7906.0 24 8100.0 25 9264.0 26 14017.0 27 17606.0 28 21314.0 29 25022.0 30 30642.0 31 36262.0 32 43186.0 33 50110.0 34 56466.5 35 62823.0 36 58670.5 37 54518.0 38 83040.0 39 111562.0 40 164886.0 41 218210.0 42 268237.5 43 318265.0 44 290503.0 45 262741.0 46 229859.0 47 196977.0 48 165926.5 49 134876.0 50 110036.5 51 85197.0 52 66845.5 53 48494.0 54 36030.5 55 23567.0 56 18567.0 57 13567.0 58 11815.5 59 10064.0 60 8992.5 61 7921.0 62 6942.5 63 5964.0 64 5072.5 65 4181.0 66 3554.5 67 2928.0 68 2383.0 69 1838.0 70 1555.0 71 1272.0 72 1120.0 73 968.0 74 767.0 75 471.5 76 377.0 77 266.0 78 155.0 79 115.0 80 75.0 81 49.5 82 24.0 83 17.5 84 11.0 85 7.0 86 3.0 87 3.0 88 3.0 89 2.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1807473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.16915729236089 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34536386303596 12.99116826013397 2 8.392171822928022 2.713886924588844 3 2.913919275822199 1.4134685732403443 4 1.4274214400253784 0.9232080714503453 5 0.8666156370119299 0.7006222273432713 6 0.6152621656437823 0.596896243939975 7 0.4589109075387659 0.5194141853022074 8 0.3618926591540741 0.4681199463050377 9 0.2827283096517811 0.4114330658786668 >10 2.7353679082118814 9.501578061301972 >50 0.46931573302301505 5.282965176317645 >100 0.8688868690594611 36.41278923720441 >500 0.24221638698573333 23.600492018384532 >1k 0.019583452564803972 4.118367352384549 >5k 3.435693432421749E-4 0.3455906562242433 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6221 0.3441821814212439 No Hit TTTAACGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTAT 2139 0.11834201672722082 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1951 0.10794075485498261 No Hit GTTGAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 1819 0.10063774120000685 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03712365274612677 0.0 2 0.0 0.0 0.0 0.13029240270809025 0.0 3 0.0 0.0 0.0 0.18583956717472405 0.0 4 0.0 0.0 0.0 0.2812766774386118 0.0 5 0.0 0.0 0.0 0.4766876185702359 0.0 6 0.0 0.0 0.0 0.6686130304574398 0.0 7 0.0 0.0 0.0 0.8010631417454092 0.0 8 0.0 0.0 0.0 1.1621750366395516 0.0 9 0.0 0.0 0.0 1.3154829975330198 0.0 10 0.0 0.0 0.0 1.5812684338853193 0.0 11 0.0 0.0 0.0 1.816016062203972 0.0 12 0.0 0.0 0.0 2.03870265281971 0.0 13 0.0 0.0 0.0 2.121968073658638 0.0 14 0.0 0.0 0.0 2.1501842627801357 0.0 15 0.0 0.0 0.0 2.2006414480326955 0.0 16 0.0 0.0 0.0 2.319868678536277 0.0 17 0.0 0.0 0.0 2.460949624143763 0.0 18 0.0 0.0 0.0 2.7135675055726973 0.0 19 0.0 0.0 0.0 2.8002078039340006 0.0 20 0.0 0.0 0.0 2.8954789366148206 0.0 21 0.0 0.0 0.0 3.015923336060898 0.0 22 0.0 0.0 0.0 3.131222430431879 0.0 23 0.0 0.0 0.0 3.2775040069754846 0.0 24 0.0 0.0 0.0 3.3847808514981965 0.0 25 0.0 0.0 0.0 3.4704806102221166 0.0 26 0.0 0.0 0.0 3.564645225682486 0.0 27 0.0 0.0 0.0 3.6527239964303755 0.0 28 0.0 0.0 0.0 3.743679711951437 0.0 29 0.0 0.0 0.0 3.8485222185891574 0.0 30 0.0 0.0 0.0 4.007639394889993 0.0 31 0.0 0.0 0.0 4.158236388593356 0.0 32 0.0 0.0 0.0 4.27707633806978 0.0 33 0.0 0.0 0.0 4.396801501322565 0.0 34 0.0 0.0 0.0 4.534784198712789 0.0 35 0.0 0.0 0.0 4.731799589814067 0.0 36 0.0 0.0 0.0 4.874927592279387 0.0 37 0.0 0.0 0.0 5.024252091179232 0.0 38 0.0 0.0 0.0 5.162234788569456 0.0 39 0.0 0.0 0.0 5.299332272183319 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 115 0.0 45.000004 25 ACTATCA 30 2.1661945E-6 45.000004 37 ACGCCTA 30 2.1661945E-6 45.000004 35 CAAGTCG 75 0.0 45.000004 1 GTTTACG 135 0.0 45.000004 1 TTGTCGA 75 0.0 45.000004 26 ACGGGTA 150 0.0 45.000004 5 ATCGTAG 45 3.8562575E-10 45.000004 1 CACCTGG 75 0.0 45.000004 2 AGCGCAT 30 2.1661945E-6 45.000004 41 TATTACG 60 0.0 45.000004 1 TCCCGTA 30 2.1661945E-6 45.000004 30 GGTTGCG 45 3.8562575E-10 45.000004 1 TACTAAG 115 0.0 45.000004 1 TCGACAC 30 2.1661945E-6 45.000004 34 GTTATCG 115 0.0 45.000004 1 TACCGGT 60 0.0 45.000004 19 CGGACAA 20 7.0343615E-4 45.0 31 CAGCGCG 80 0.0 45.0 1 CCTTACG 20 7.0343615E-4 45.0 43 >>END_MODULE