FastQCFastQC Report
Thu 26 May 2016
SRR1545152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545152_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2130646
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82730.3882859940130834No Hit
AATTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA25210.11832092238691927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22870.10733833776235002No Hit
GAAATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA22580.10597724821486065No Hit
AGTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA21310.10001661467930383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG302.1663782E-645.0000041
CTATGCG351.2125747E-745.0000041
GGTACGT253.8919305E-545.0000048
CGTGCAA253.8919305E-545.00000411
CGCATCG351.2125747E-745.00000429
ATTCGCA253.8919305E-545.00000433
TACGGGT453.8562575E-1045.0000044
AATATCG302.1663782E-645.00000430
ACGGCAA253.8919305E-545.00000436
ATCCCGC253.8919305E-545.00000423
CCATACG253.8919305E-545.00000443
GTCGTTC351.2125747E-745.00000417
CTACCGT302.1663782E-645.00000421
GTTCACG453.8562575E-1045.0000041
ACCGTTG253.8919305E-545.00000410
TAAATCG253.8919305E-545.00000431
TCGACTG600.045.0000041
TCGACTA253.8919305E-545.00000435
TAGTCCG253.8919305E-545.0000041
ATCGACT253.8919305E-545.00000434