##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545152_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2130646
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.828706880448465	31.0	31.0	33.0	28.0	33.0
2	31.150231432157195	31.0	31.0	33.0	30.0	34.0
3	31.28839704014651	31.0	31.0	34.0	28.0	34.0
4	35.149398351485885	35.0	35.0	37.0	32.0	37.0
5	35.008365537963606	35.0	35.0	37.0	32.0	37.0
6	35.09289107622759	37.0	35.0	37.0	32.0	37.0
7	35.538013353696485	36.0	35.0	37.0	35.0	37.0
8	35.62227042878075	37.0	35.0	37.0	35.0	37.0
9	37.48864428910293	39.0	37.0	39.0	35.0	39.0
10	36.95522954071207	39.0	37.0	39.0	32.0	39.0
11	36.475736466780496	38.0	35.0	39.0	32.0	39.0
12	35.06338359352046	35.0	34.0	39.0	30.0	39.0
13	34.571916686300774	35.0	33.0	39.0	27.0	39.0
14	35.19253785002295	35.0	33.0	40.0	27.0	41.0
15	35.674729635988335	35.0	34.0	40.0	30.0	41.0
16	35.879084559330835	35.0	34.0	40.0	31.0	41.0
17	35.73888060240885	35.0	34.0	40.0	31.0	41.0
18	35.81905159280331	36.0	35.0	39.0	31.0	41.0
19	35.67396648715929	36.0	34.0	39.0	31.0	41.0
20	35.408402428183756	35.0	34.0	39.0	30.0	41.0
21	35.236344751779505	35.0	34.0	39.0	30.0	41.0
22	35.06956669479585	35.0	34.0	39.0	29.0	41.0
23	34.99931616983769	35.0	34.0	39.0	29.0	41.0
24	35.14249575011522	35.0	34.0	39.0	30.0	41.0
25	35.064999535352186	35.0	34.0	39.0	30.0	41.0
26	34.81948197870505	35.0	34.0	39.0	29.0	41.0
27	34.80124619481603	35.0	34.0	39.0	29.0	41.0
28	34.89111799895431	35.0	34.0	39.0	29.0	41.0
29	34.889436818692545	36.0	34.0	39.0	29.0	41.0
30	34.84915795491133	36.0	34.0	39.0	29.0	41.0
31	34.600010513243404	35.0	34.0	39.0	29.0	41.0
32	34.40919983892209	35.0	34.0	39.0	27.0	41.0
33	34.14061697719846	35.0	33.0	39.0	26.0	41.0
34	33.910675917069284	35.0	33.0	39.0	24.0	41.0
35	33.67355158951792	35.0	33.0	39.0	23.0	41.0
36	33.427272761406634	35.0	33.0	39.0	23.0	41.0
37	33.44881364618993	35.0	33.0	39.0	23.0	41.0
38	33.50724756716977	35.0	33.0	39.0	23.0	41.0
39	33.5042996349464	35.0	33.0	39.0	23.0	41.0
40	33.14357523492875	35.0	32.0	39.0	23.0	41.0
41	33.2371210421628	35.0	32.0	39.0	23.0	41.0
42	33.23679625803629	35.0	32.0	39.0	23.0	41.0
43	33.20784306731386	35.0	32.0	39.0	23.0	41.0
44	33.180065576355716	35.0	32.0	39.0	23.0	41.0
45	33.236183767739924	35.0	33.0	39.0	23.0	41.0
46	33.25536339682894	35.0	33.0	39.0	23.0	41.0
47	33.19007709398934	35.0	32.0	39.0	23.0	41.0
48	33.24194680862048	35.0	33.0	39.0	23.0	41.0
49	33.30236369626864	35.0	33.0	39.0	23.0	41.0
50	33.07545176439446	35.0	32.0	38.0	23.0	41.0
51	32.77526675008425	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	4.0
13	5.0
14	14.0
15	53.0
16	180.0
17	571.0
18	1442.0
19	2822.0
20	5058.0
21	8102.0
22	12503.0
23	18377.0
24	25552.0
25	34789.0
26	44168.0
27	49764.0
28	53845.0
29	61059.0
30	72659.0
31	88880.0
32	113333.0
33	148808.0
34	270079.0
35	393874.0
36	117327.0
37	141971.0
38	198408.0
39	266916.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.40289189288131	25.93894058421718	29.605903561642805	14.0522639612587
2	29.05147077459137	26.841342954202624	32.451847937198394	11.65533833400762
3	25.408772738408913	25.352874198717195	36.563652526041395	12.674700536832493
4	23.455890842495656	28.545520935903944	35.67706695528023	12.321521266320168
5	21.36272285494634	33.25352968066962	33.64646215279309	11.737285311590945
6	19.006066704652017	41.8450554432787	29.817904992194855	9.330972859874423
7	87.64994278730488	4.118563102458127	7.27943543882935	0.9520586714076388
8	90.39680923062771	2.193794745818874	6.5510647944332385	0.8583312291201824
9	87.3659913472252	3.559671573785603	7.641579126706173	1.4327579522830165
10	60.74932203660298	22.46023037144603	11.336233236304857	5.454214355646128
11	55.07813123343812	15.049191653611157	21.29147685725362	8.581200255697098
12	50.32445558764807	20.468956363469108	20.738545962116653	8.468042086766173
13	17.793758324939947	53.02851811140846	20.396724749207518	8.78099881444407
14	10.063755311769293	52.87828198583904	26.970834197703418	10.087128504688248
15	8.549378920759244	22.957168858646625	58.62606927664192	9.86738294395221
16	9.170927502738605	17.050134090787488	57.15430906870499	16.62462933776892
17	10.155605389163663	16.635564988271163	32.07435679132057	41.13447283124461
18	15.541765267435323	21.764056534966393	45.95033618911823	16.743842008480055
19	31.893097210892847	19.697359392409624	30.022115358440587	18.387428038256942
20	32.60626119965494	19.67788173164383	31.0810430263873	16.634814042313927
21	16.489928406689803	33.946934403931955	32.633952331828	16.929184857550243
22	18.805564134070135	24.190879198139907	22.850346796229875	34.15320987156008
23	11.094381703952697	34.47128241857165	26.638399809259727	27.79593606821593
24	9.811108931281874	20.742723099003776	56.81098596388138	12.635182005832974
25	9.56982060839764	24.36110925982073	49.872573857881605	16.19649627390003
26	9.95177049589655	40.81128446489938	31.486929316273095	17.75001572293098
27	9.534338411918263	50.87949851828976	26.666654150900715	12.919508918891264
28	8.362581113896912	34.78893255848226	43.401156269037656	13.447330058583171
29	9.268456608934567	24.63689416261547	42.86939266307026	23.225256565379702
30	13.548426158076001	42.04086460162787	31.08996050962947	13.320748730666661
31	31.30383930507461	30.107582395198452	27.33095971832017	11.257618581406765
32	35.43836000912399	21.083887234200333	31.067619867401717	12.410132889273957
33	36.475087837209934	26.750666229866432	21.676524396826128	15.097721536097502
34	19.84412239292684	25.498651582665538	24.805199925280878	29.852026099126743
35	17.753488847983192	24.201110836807242	36.34165412743366	21.703746187775913
36	37.54157189885133	25.754442549348884	24.442821566792418	12.261163985007364
37	15.792064941806384	44.49232767902317	27.768338804287524	11.947268574882923
38	15.940470636605047	42.90553193726222	23.660007340496733	17.493990085636
39	19.31118543390127	41.23852578044405	26.428651216579386	13.021637569075295
40	28.159393911517917	23.785696920089023	25.38385071945316	22.6710584489399
41	13.615776623615561	24.53377989586257	28.26635677630165	33.584086704220226
42	16.10530327421824	20.458536988312463	27.16739430200981	36.268765435459486
43	16.689633097192118	20.72629615618925	30.169253831936416	32.41481691468221
44	11.531666921675399	30.077826161642996	36.16949976673741	22.221007149944192
45	11.551989396643084	52.080354972153984	22.44539918879063	13.922256442412301
46	18.0443865381673	42.29599849059863	24.66388128295362	14.995733688280454
47	16.596234193760953	30.289639855705737	25.839674915495113	27.2744510350382
48	17.92315570019609	26.974166520388653	39.55626603386954	15.546411745545718
49	22.486842018805564	25.36174474783704	38.871872662094034	13.279540571263363
50	18.471580919589645	43.03338048648156	25.056719886832447	13.438318707096345
51	15.198066689633096	39.946476326898036	25.415859790880326	19.43959719258854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2647.0
1	3108.0
2	3569.0
3	9891.5
4	16214.0
5	13191.5
6	10169.0
7	10814.5
8	11460.0
9	12354.0
10	13248.0
11	13477.5
12	13707.0
13	13329.5
14	12952.0
15	12188.0
16	11424.0
17	10775.5
18	10127.0
19	9503.0
20	8879.0
21	9770.0
22	10661.0
23	10914.5
24	11168.0
25	14939.5
26	22602.0
27	26493.0
28	26992.0
29	27491.0
30	37666.0
31	47841.0
32	58314.0
33	68787.0
34	78725.5
35	88664.0
36	97693.0
37	106722.0
38	120615.0
39	134508.0
40	190995.0
41	247482.0
42	289162.5
43	330843.0
44	322214.0
45	313585.0
46	261273.0
47	208961.0
48	180383.5
49	151806.0
50	123539.5
51	95273.0
52	72834.0
53	50395.0
54	38054.0
55	25713.0
56	20462.5
57	15212.0
58	12991.5
59	10771.0
60	9518.0
61	8265.0
62	6980.5
63	5696.0
64	4800.0
65	3904.0
66	3243.0
67	2582.0
68	2094.5
69	1607.0
70	1475.5
71	1344.0
72	1038.0
73	732.0
74	627.5
75	403.0
76	283.0
77	188.5
78	94.0
79	88.0
80	82.0
81	58.0
82	34.0
83	21.0
84	8.0
85	7.5
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2130646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.556166956098725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.66891177145587	14.162368832264768
2	7.835627480497701	2.751271685068257
3	2.7642036672754333	1.4558646325004363
4	1.4508567549670002	1.018859336783371
5	0.9081238604116687	0.7971587055102074
6	0.6549207035609984	0.6898738328833517
7	0.49467691702728384	0.6079241381261433
8	0.37655352101922873	0.5288669186336328
9	0.31530549090869475	0.4982000256510941
>10	2.9961736546945033	11.391977478168116
>50	0.5304297359701544	6.6049513550079375
>100	0.790541946624714	34.323829186758395
>500	0.1981052484209434	21.187050847631294
>1k	0.01530081186991026	3.5919217781828174
>5k	2.684352959633379E-4	0.38988124683022973
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8273	0.3882859940130834	No Hit
AATTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2521	0.11832092238691927	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2287	0.10733833776235002	No Hit
GAAATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2258	0.10597724821486065	No Hit
AGTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2131	0.10001661467930383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0191960560318326	0.0
2	0.0	0.0	0.0	0.07241935075089902	0.0
3	0.0	0.0	0.0	0.10649352356046007	0.0
4	0.0	0.0	0.0	0.17877207194437744	0.0
5	0.0	0.0	0.0	0.3344994898260903	0.0
6	0.0	0.0	0.0	0.4910247877873659	0.0
7	0.0	0.0	0.0	0.6049808367978537	0.0
8	0.0	0.0	0.0	0.9258224970267234	0.0
9	0.0	0.0	0.0	1.0621191882649674	0.0
10	0.0	0.0	0.0	1.29768154822528	0.0
11	0.0	0.0	0.0	1.473168231606752	0.0
12	0.0	0.0	0.0	1.6457919335262639	0.0
13	0.0	0.0	0.0	1.7078857773651748	0.0
14	0.0	0.0	0.0	1.7293816053910411	0.0
15	0.0	0.0	0.0	1.7646760653811098	0.0
16	0.0	0.0	0.0	1.8404277388172414	0.0
17	0.0	0.0	0.0	1.9377691085238937	0.0
18	0.0	0.0	0.0	2.1058402005776653	0.0
19	0.0	0.0	0.0	2.17394161207446	0.0
20	0.0	0.0	0.0	2.249740219632919	0.0
21	0.0	0.0	0.0	2.3543563783002903	0.0
22	0.0	0.0	0.0	2.4596296146802423	0.0
23	0.0	0.0	0.0	2.593016390334199	0.0
24	0.0	0.0	0.0	2.6933146097474663	0.0
25	0.0	0.0	0.0	2.7752146532084634	0.0
26	0.0	0.0	0.0	2.862512120737091	0.0
27	0.0	0.0	0.0	2.9478852892503027	0.0
28	0.0	0.0	0.0	3.039641498400016	0.0
29	0.0	0.0	0.0	3.1474961115079654	0.0
30	0.0	0.0	0.0	3.3027072540440785	0.0
31	0.0	0.0	0.0	3.442477070334537	0.0
32	0.0	0.0	0.0	3.559859310274912	0.0
33	0.0	0.0	0.0	3.679869861065611	0.0
34	0.0	0.0	0.0	3.80748373967332	0.0
35	0.0	0.0	0.0	3.997191462119939	0.0
36	0.0	0.0	0.0	4.1387917091811595	0.0
37	0.0	0.0	0.0	4.28780754757008	0.0
38	0.0	0.0	0.0	4.4313320936467155	0.0
39	0.0	0.0	0.0	4.5833047817422505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	30	2.1663782E-6	45.000004	1
CTATGCG	35	1.2125747E-7	45.000004	1
GGTACGT	25	3.8919305E-5	45.000004	8
CGTGCAA	25	3.8919305E-5	45.000004	11
CGCATCG	35	1.2125747E-7	45.000004	29
ATTCGCA	25	3.8919305E-5	45.000004	33
TACGGGT	45	3.8562575E-10	45.000004	4
AATATCG	30	2.1663782E-6	45.000004	30
ACGGCAA	25	3.8919305E-5	45.000004	36
ATCCCGC	25	3.8919305E-5	45.000004	23
CCATACG	25	3.8919305E-5	45.000004	43
GTCGTTC	35	1.2125747E-7	45.000004	17
CTACCGT	30	2.1663782E-6	45.000004	21
GTTCACG	45	3.8562575E-10	45.000004	1
ACCGTTG	25	3.8919305E-5	45.000004	10
TAAATCG	25	3.8919305E-5	45.000004	31
TCGACTG	60	0.0	45.000004	1
TCGACTA	25	3.8919305E-5	45.000004	35
TAGTCCG	25	3.8919305E-5	45.000004	1
ATCGACT	25	3.8919305E-5	45.000004	34
>>END_MODULE