FastQCFastQC Report
Thu 26 May 2016
SRR1545150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545150_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2167284
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71160.32833721838023994No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG51400.23716319596324248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21850.10081742863417992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGA302.1663946E-645.0000044
CCGTACC750.045.00000431
TCTACGG302.1663946E-645.0000042
CGCTATA302.1663946E-645.00000418
TATCGTG650.045.0000041
CCGCAAC302.1663946E-645.00000413
TGCACCG453.8562575E-1045.00000416
ACGACGA453.8562575E-1045.0000041
TACGTAA650.045.0000041
CGGAATA351.2125929E-745.034
TCGTTAG253.8919534E-545.01
GTCGAAA253.8919534E-545.042
CAACGAA253.8919534E-545.08
CGTGCCG207.034673E-445.040
CGCATTG253.8919534E-545.037
TCGGGTG850.045.05
TTATCCG253.8919534E-545.01
CCGTAGA207.034673E-445.028
GTATGCG351.2125929E-745.01
CACTAGC351.2125929E-745.031