##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545150_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2167284
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83817995241971	31.0	31.0	33.0	30.0	33.0
2	31.149626444896008	31.0	31.0	33.0	30.0	34.0
3	31.29341378425716	31.0	31.0	34.0	28.0	34.0
4	35.16943741567787	35.0	35.0	37.0	32.0	37.0
5	35.02707859237645	35.0	35.0	37.0	32.0	37.0
6	35.11613752512361	37.0	35.0	37.0	32.0	37.0
7	35.50689434333479	36.0	35.0	37.0	35.0	37.0
8	35.57023629575081	37.0	35.0	37.0	35.0	37.0
9	37.40560443393667	39.0	37.0	39.0	35.0	39.0
10	36.89058471340166	39.0	37.0	39.0	32.0	39.0
11	36.48630174910164	38.0	35.0	39.0	32.0	39.0
12	35.07345830080414	35.0	34.0	39.0	30.0	39.0
13	34.602810245450065	35.0	33.0	39.0	28.0	39.0
14	35.250124118481935	35.0	33.0	40.0	27.0	41.0
15	35.715344181934626	35.0	34.0	40.0	30.0	41.0
16	35.91935436241859	35.0	34.0	40.0	31.0	41.0
17	35.786955931940625	35.0	34.0	40.0	31.0	41.0
18	35.89449744472805	36.0	35.0	40.0	31.0	41.0
19	35.780723707645144	36.0	35.0	40.0	31.0	41.0
20	35.48757107974774	35.0	34.0	40.0	30.0	41.0
21	35.341897047179785	35.0	34.0	40.0	30.0	41.0
22	35.14842263404335	35.0	34.0	40.0	29.0	41.0
23	35.09470286312269	35.0	34.0	39.0	29.0	41.0
24	35.22211902085744	35.0	34.0	39.0	30.0	41.0
25	35.13931953541852	35.0	34.0	39.0	30.0	41.0
26	34.89097229527833	35.0	34.0	39.0	29.0	41.0
27	34.88617273970555	35.0	34.0	39.0	29.0	41.0
28	34.985314338130124	36.0	34.0	39.0	29.0	41.0
29	34.97628691025265	36.0	34.0	39.0	29.0	41.0
30	34.93870854027437	36.0	34.0	39.0	29.0	41.0
31	34.686052220198185	35.0	34.0	39.0	29.0	41.0
32	34.53826586640237	35.0	34.0	39.0	28.0	41.0
33	34.30682642422497	35.0	33.0	39.0	27.0	41.0
34	34.11695144706462	35.0	33.0	39.0	25.0	41.0
35	33.92291504020701	35.0	33.0	39.0	24.0	41.0
36	33.644906251326546	35.0	33.0	39.0	23.0	41.0
37	33.70643210580616	35.0	33.0	39.0	23.0	41.0
38	33.77833177377769	35.0	33.0	39.0	24.0	41.0
39	33.76181201909856	35.0	33.0	39.0	23.0	41.0
40	33.46354607887107	35.0	32.0	39.0	23.0	41.0
41	33.53783768071005	35.0	33.0	39.0	23.0	41.0
42	33.53843335714193	35.0	33.0	39.0	23.0	41.0
43	33.50245791506789	35.0	33.0	39.0	23.0	41.0
44	33.48244853927773	35.0	33.0	39.0	23.0	41.0
45	33.53831938961391	35.0	33.0	39.0	23.0	41.0
46	33.56171226290601	35.0	33.0	39.0	23.0	41.0
47	33.47932112265859	35.0	33.0	39.0	23.0	41.0
48	33.53258363924617	35.0	33.0	39.0	23.0	41.0
49	33.57845395435024	35.0	33.0	39.0	24.0	41.0
50	33.36992936781704	35.0	33.0	39.0	24.0	41.0
51	33.03639163118447	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	7.0
14	19.0
15	56.0
16	184.0
17	489.0
18	1231.0
19	2609.0
20	4944.0
21	7672.0
22	11689.0
23	16553.0
24	23303.0
25	32371.0
26	41297.0
27	48004.0
28	53294.0
29	60997.0
30	73109.0
31	89845.0
32	113899.0
33	152036.0
34	276412.0
35	397672.0
36	119860.0
37	145306.0
38	206473.0
39	287862.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.345868838601675	25.150049555111377	30.508507422192938	12.995574184094009
2	31.04715394936704	26.0254770486932	31.043462693398745	11.883906308541013
3	27.271091375195866	25.955112481797492	34.3386007556001	12.435195387406543
4	25.812122453725493	28.79410358771624	32.73576513276525	12.658008825793022
5	22.068035384379712	32.958809274649745	32.27989502068026	12.693260320290282
6	19.224061082903763	42.765876553326656	29.123963449183403	8.886098914586182
7	87.31495272423918	4.748339396221262	6.707565782795425	1.2291420967441278
8	88.81734004403668	3.2440603077400096	6.6159303533823905	1.322669294840916
9	84.73471866169824	5.433113519040421	7.883323090098021	1.9488447291633213
10	59.845594762846034	20.11388447476196	13.64218994834087	6.3983308140511355
11	57.088687961522346	16.32762480597836	18.59511720660513	7.988570025894161
12	51.311226401339184	21.155049361320437	19.613211743361738	7.920512493978639
13	17.92040175629959	53.83046245900399	18.632306610485752	9.616829174210672
14	9.857176078446573	53.30478146841854	25.623499273745388	11.214543179389503
15	8.719392566917856	22.70210087833436	57.96508440979585	10.613422144951931
16	9.319452365264542	16.566818192724163	56.072254489951476	18.041474952059815
17	9.419116276408628	16.94291103519428	31.40303716541072	42.23493552298637
18	15.085471031945975	21.79502086482436	45.562049090013126	17.55745901321654
19	32.82204824102425	19.89706932732397	27.83880654312033	19.442075888531452
20	32.40461333170918	20.27200865230399	30.867943472106102	16.455434543880727
21	16.041367905636733	35.28610002196297	31.011302625774935	17.661229446625363
22	19.687590551122973	24.637749367411008	21.013351272837337	34.66130880862868
23	12.486227001168281	34.3141000441105	26.04282595174421	27.156847002976995
24	9.93935266444084	21.039559190212266	56.687863704064625	12.333224441282269
25	9.552278335465033	25.26152548535402	49.12752551119281	16.058670667988135
26	9.292967603691995	42.80546527358666	28.78104576972838	19.120521352992963
27	9.505630088165649	52.018286482066955	25.65136825630605	12.824715173461346
28	8.53653697438822	35.00967109063694	42.881781990731255	13.572009944243579
29	8.853477439966335	25.6446317141639	41.79890591173099	23.702984934138765
30	13.700050385643966	44.00715365406656	29.088388969788916	13.204406990500553
31	31.74078708651012	31.281871688251282	26.32926741488425	10.648073810354342
32	36.065324156871	21.959143333314877	29.784421423311393	12.191111086502739
33	35.87407095701348	27.843420613080706	20.39834188781904	15.884166542086778
34	18.284359594773917	26.290416945817903	25.21252406237484	30.212699397033337
35	18.50371248069012	22.964641459079658	36.507398199774464	22.02424786045576
36	37.999450002860726	26.642147498897238	22.978668231759197	12.379734266482842
37	15.10535767347519	44.81069393766576	28.59879000629359	11.485158382565459
38	14.754411512289115	43.46075548935903	23.961788118216163	17.82304488013569
39	18.851521074303136	40.82367608490627	27.135483859060468	13.189318981730128
40	28.007127815274785	24.45318656899603	24.68398234841396	22.85570326731522
41	12.969273985319877	23.777779008196433	29.030620813885026	34.222326192598665
42	15.102173965202532	20.710576002037573	27.092849852626603	37.09440018013329
43	15.711046637173531	20.88245010806152	30.720293233374125	32.68621002139083
44	11.51413474191661	30.673737267473943	35.43961012954463	22.372517861064818
45	10.790971557027136	52.701261117601575	22.129863921848727	14.377903403522566
46	18.332668907259038	43.43168684860868	22.767389968273655	15.468254275858632
47	16.393975132008542	30.86812803490452	25.281135282685614	27.456761550401332
48	16.996803372331453	28.126909071446104	40.09663708125008	14.77965047497236
49	22.214255261423975	27.05446079055629	38.20952860815657	12.521755339863166
50	19.036083872718113	43.994095836078706	24.25141328962886	12.71840700157432
51	15.278108452791606	39.54936224325007	24.879988040330662	20.292541263627655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2743.0
1	3642.0
2	4541.0
3	9895.0
4	15249.0
5	12135.5
6	9022.0
7	9617.0
8	10212.0
9	11047.0
10	11882.0
11	11781.0
12	11680.0
13	11355.5
14	11031.0
15	10355.0
16	9679.0
17	9322.0
18	8965.0
19	9196.0
20	9427.0
21	9767.0
22	10107.0
23	10639.0
24	11171.0
25	12514.5
26	19138.0
27	24418.0
28	33126.0
29	41834.0
30	49349.5
31	56865.0
32	66003.0
33	75141.0
34	84309.0
35	93477.0
36	98061.5
37	102646.0
38	119652.0
39	136658.0
40	188984.5
41	241311.0
42	304561.0
43	367811.0
44	327600.5
45	287390.0
46	248147.0
47	208904.0
48	175602.5
49	142301.0
50	121006.5
51	99712.0
52	74222.5
53	48733.0
54	37271.0
55	25809.0
56	21557.5
57	17306.0
58	15774.0
59	14242.0
60	13313.5
61	12385.0
62	11029.0
63	9673.0
64	8247.5
65	6822.0
66	5727.5
67	4633.0
68	3865.5
69	3098.0
70	2814.5
71	2531.0
72	2146.5
73	1762.0
74	1435.0
75	815.0
76	522.0
77	440.0
78	358.0
79	257.0
80	156.0
81	108.0
82	60.0
83	46.5
84	33.0
85	21.0
86	9.0
87	7.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2167284.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.931545990377979
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4462391885204	12.8163295938486
2	7.895948304971689	2.5158932711660706
3	2.7848851474430796	1.3310257741322995
4	1.4535820926821024	0.9263123984141913
5	0.941920989169828	0.7503128779130718
6	0.6749639869243704	0.6451931879720694
7	0.4873476105937865	0.543494061003301
8	0.41155074337492153	0.5245311676361447
9	0.34157054513578333	0.4897572163910289
>10	2.912562168851017	9.495362714308914
>50	0.45346255803369195	5.1394298086814665
>100	0.9786766315339347	41.23565128802286
>500	0.19782688811930166	19.129081078209953
>1k	0.01888215525367784	3.890414252975182
>5k	5.809893924208565E-4	0.5672113093248298
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7116	0.32833721838023994	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5140	0.23716319596324248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2185	0.10081742863417992	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019794360130006034	0.0
2	0.0	0.0	0.0	0.08116149060298512	0.0
3	0.0	0.0	0.0	0.11613614090262282	0.0
4	0.0	0.0	0.0	0.17321218631245375	0.0
5	0.0	0.0	0.0	0.2994531404282964	0.0
6	0.0	0.0	0.0	0.42057247688812355	0.0
7	0.0	0.0	0.0	0.5115619365067061	0.0
8	0.0	0.0	0.0	0.741434901932557	0.0
9	0.0	0.0	0.0	0.8275795880927465	0.0
10	0.0	0.0	0.0	0.9629102600305267	0.0
11	0.0	0.0	0.0	1.0984716354663255	0.0
12	0.0	0.0	0.0	1.2229131022976223	0.0
13	0.0	0.0	0.0	1.2712685554823457	0.0
14	0.0	0.0	0.0	1.2896325539246356	0.0
15	0.0	0.0	0.0	1.3203161191611252	0.0
16	0.0	0.0	0.0	1.3886966359738733	0.0
17	0.0	0.0	0.0	1.4677356543950861	0.0
18	0.0	0.0	0.0	1.6076803962932407	0.0
19	0.0	0.0	0.0	1.6633260800153555	0.0
20	0.0	0.0	0.0	1.7234935522986374	0.0
21	0.0	0.0	0.0	1.8074234848778472	0.0
22	0.0	0.0	0.0	1.8935220303384328	0.0
23	0.0	0.0	0.0	2.002783207000098	0.0
24	0.0	0.0	0.0	2.0859287476860437	0.0
25	0.0	0.0	0.0	2.1517253853209826	0.0
26	0.0	0.0	0.0	2.225965770983406	0.0
27	0.0	0.0	0.0	2.2945769912941727	0.0
28	0.0	0.0	0.0	2.3636034779013735	0.0
29	0.0	0.0	0.0	2.447764113978602	0.0
30	0.0	0.0	0.0	2.5674069480511093	0.0
31	0.0	0.0	0.0	2.6771756724084153	0.0
32	4.614069960374367E-5	0.0	0.0	2.7774394126473503	0.0
33	4.614069960374367E-5	0.0	0.0	2.8749808516096644	0.0
34	4.614069960374367E-5	0.0	0.0	2.9784744408208614	0.0
35	4.614069960374367E-5	0.0	0.0	3.126401523750464	0.0
36	4.614069960374367E-5	0.0	0.0	3.238846408684787	0.0
37	4.614069960374367E-5	0.0	0.0	3.3541981576941464	0.0
38	4.614069960374367E-5	0.0	0.0	3.4686270927114307	0.0
39	4.614069960374367E-5	0.0	0.0	3.577611425175473	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGA	30	2.1663946E-6	45.000004	4
CCGTACC	75	0.0	45.000004	31
TCTACGG	30	2.1663946E-6	45.000004	2
CGCTATA	30	2.1663946E-6	45.000004	18
TATCGTG	65	0.0	45.000004	1
CCGCAAC	30	2.1663946E-6	45.000004	13
TGCACCG	45	3.8562575E-10	45.000004	16
ACGACGA	45	3.8562575E-10	45.000004	1
TACGTAA	65	0.0	45.000004	1
CGGAATA	35	1.2125929E-7	45.0	34
TCGTTAG	25	3.8919534E-5	45.0	1
GTCGAAA	25	3.8919534E-5	45.0	42
CAACGAA	25	3.8919534E-5	45.0	8
CGTGCCG	20	7.034673E-4	45.0	40
CGCATTG	25	3.8919534E-5	45.0	37
TCGGGTG	85	0.0	45.0	5
TTATCCG	25	3.8919534E-5	45.0	1
CCGTAGA	20	7.034673E-4	45.0	28
GTATGCG	35	1.2125929E-7	45.0	1
CACTAGC	35	1.2125929E-7	45.0	31
>>END_MODULE