FastQCFastQC Report
Thu 26 May 2016
SRR1545149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545149_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1097067
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.6301347137412756No Hit
TTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA11660.10628339016668992No Hit
GGCCTGGGCAGTGTAGTCTTAGCAACTGGTGTATATTTTTATATGGTGTTT11170.10181693551989075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG207.033145E-445.00000421
CAGCGAA207.033145E-445.00000433
CTCACGA406.8139343E-945.00000424
AGTCCGT207.033145E-445.00000432
CGTATTC750.045.00000429
CGTGCCT207.033145E-445.00000433
GCGCGAT207.033145E-445.0000043
CGGTTTA207.033145E-445.00000417
GACCGTT406.8139343E-945.0000049
GGCGTAA302.1654105E-645.00000434
GCACGAG302.1654105E-645.0000041
CGCGATA207.033145E-445.0000044
TAAGTCG406.8139343E-945.0000041
TACGGGC406.8139343E-945.0000044
ACTTACG207.033145E-445.00000443
ATCGTAG600.045.0000041
CCGTACG207.033145E-445.00000435
CACTCGA207.033145E-445.00000410
TGCGCGA207.033145E-445.0000042
CGTAGTA207.033145E-445.00000418