##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545149_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1097067
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.104163191491494	31.0	31.0	33.0	30.0	33.0
2	31.43460791364611	31.0	31.0	33.0	30.0	34.0
3	31.563681160767757	31.0	31.0	34.0	30.0	34.0
4	35.44511957792915	37.0	35.0	37.0	33.0	37.0
5	35.38836734675275	37.0	35.0	37.0	33.0	37.0
6	35.48023958427334	37.0	35.0	37.0	33.0	37.0
7	35.69493020936734	37.0	35.0	37.0	35.0	37.0
8	35.74825238567927	37.0	35.0	37.0	35.0	37.0
9	37.65167943252327	39.0	37.0	39.0	35.0	39.0
10	37.241466564940886	39.0	37.0	39.0	34.0	39.0
11	36.8905618344185	39.0	35.0	39.0	33.0	39.0
12	35.6076930579445	37.0	35.0	39.0	32.0	39.0
13	35.1842321389669	35.0	35.0	39.0	31.0	39.0
14	35.863215282202454	36.0	35.0	40.0	31.0	41.0
15	36.23241880395637	37.0	35.0	40.0	32.0	41.0
16	36.40715197886729	36.0	35.0	40.0	32.0	41.0
17	36.2897297977243	36.0	35.0	40.0	32.0	41.0
18	36.39065891144296	36.0	35.0	40.0	32.0	41.0
19	36.266980047709026	36.0	35.0	40.0	32.0	41.0
20	35.977103495046336	36.0	35.0	40.0	31.0	41.0
21	35.86571193919788	35.0	34.0	40.0	31.0	41.0
22	35.702716424794474	35.0	34.0	40.0	31.0	41.0
23	35.654878872484545	35.0	34.0	40.0	31.0	41.0
24	35.678219288338816	35.0	34.0	40.0	31.0	41.0
25	35.58484577514409	35.0	34.0	40.0	31.0	41.0
26	35.38994883630626	35.0	34.0	40.0	31.0	41.0
27	35.38865265293733	35.0	34.0	40.0	31.0	41.0
28	35.44807290712418	36.0	34.0	40.0	31.0	41.0
29	35.46240019980548	36.0	34.0	40.0	31.0	41.0
30	35.428005764461055	36.0	34.0	40.0	30.0	41.0
31	35.175799654898015	35.0	34.0	40.0	30.0	41.0
32	35.03277283885123	35.0	34.0	40.0	29.0	41.0
33	34.77167666149834	35.0	34.0	40.0	29.0	41.0
34	34.52462338216353	35.0	34.0	40.0	27.0	41.0
35	34.27348648715165	35.0	34.0	40.0	26.0	41.0
36	34.01853852134828	35.0	33.0	40.0	24.0	41.0
37	34.0028931687855	35.0	33.0	40.0	24.0	41.0
38	34.021963107084616	35.0	33.0	40.0	25.0	41.0
39	34.04738270315304	35.0	33.0	40.0	24.0	41.0
40	33.72004991490948	35.0	33.0	40.0	23.0	41.0
41	33.81268783036952	35.0	33.0	40.0	23.0	41.0
42	33.81308525368095	35.0	33.0	40.0	23.0	41.0
43	33.774625433086584	35.0	33.0	40.0	23.0	41.0
44	33.704596893352914	35.0	33.0	39.0	23.0	41.0
45	33.74610848744881	35.0	33.0	39.0	23.0	41.0
46	33.76269270700878	35.0	33.0	39.0	23.0	41.0
47	33.73899497478276	35.0	33.0	39.0	23.0	41.0
48	33.78485908335589	35.0	33.0	39.0	23.0	41.0
49	33.791366434319876	35.0	33.0	39.0	24.0	41.0
50	33.572039811606764	35.0	33.0	39.0	24.0	41.0
51	33.27861744086733	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	1.0
14	1.0
15	21.0
16	53.0
17	152.0
18	397.0
19	839.0
20	1542.0
21	2683.0
22	4343.0
23	6873.0
24	10307.0
25	14702.0
26	19471.0
27	22563.0
28	22784.0
29	25525.0
30	30661.0
31	38948.0
32	51707.0
33	71293.0
34	139381.0
35	210701.0
36	63903.0
37	78215.0
38	116551.0
39	163368.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.627026425915645	23.468119996317455	31.02773121422848	14.877122363538417
2	31.99139159230931	25.54046380029661	31.296630014392925	11.171514593001158
3	27.44973643359977	25.066837303464602	34.99640404824865	12.487022214686979
4	24.200253949849916	28.08634294897212	33.91269630751814	13.800706793659822
5	21.252302730826834	32.323185366071534	33.75864919827139	12.665862704830245
6	18.08877671099395	41.560907401279955	30.8989332465565	9.451382641169591
7	86.25544292189994	4.283238854144733	8.26740755122522	1.1939106727301068
8	88.4847507034666	2.651251017485714	7.91875063236794	0.9452476466797378
9	84.73256419161272	4.209496776404722	9.323040434175853	1.7348985978066973
10	57.57150657161322	21.10153709846345	14.582245204714022	6.744711125209308
11	53.61477466736307	15.145838859431557	21.74780574021459	9.491580732990784
12	50.63984241618789	19.743279125158264	20.92014434852201	8.696734110131834
13	17.61177758514293	51.37288789107684	19.900151950610127	11.115182573170099
14	9.852634342296323	50.98622053165395	26.424912972498486	12.736232153551242
15	9.147754877322898	22.768709659482965	56.880117622715844	11.203417840478295
16	9.399790532392279	16.64930218482554	54.97139190222657	18.979515380555608
17	9.395688686288075	17.346433718268802	33.15431053891877	40.103567056524355
18	14.816232736924908	22.67974517508958	44.84457193589817	17.659450152087338
19	29.805289923040252	18.7695008600204	29.65261009582824	21.772599121111107
20	29.906742250017547	19.407201201020538	32.79963757910866	17.886418969853253
21	15.087501492616221	33.21994007658603	32.66281822349957	19.029740207298186
22	19.828962132668288	24.04802988331615	22.74473664780729	33.37827133620827
23	12.628672633485467	32.57540332541221	26.278431490510606	28.517492550591715
24	10.912369071351158	19.741729538852233	56.64467165633458	12.701229733462041
25	10.083066941216899	25.064011587259483	47.19666164418399	17.656259827339625
26	9.832033959639658	38.184085384028506	31.624321942050944	20.359558714280894
27	8.952780459169768	48.372250737648656	28.15169903023243	14.523269772949146
28	8.443695781570314	33.92308765098212	42.97403896024582	14.659177607201748
29	8.78843315859469	23.210888669516084	42.41017184912133	25.590506322767894
30	13.949102470496333	41.54149199638673	31.059178701027374	13.450226832089562
31	29.656803094068092	30.939587099055938	28.5580552509555	10.845554555920469
32	33.144374956133035	20.592178964457048	32.661633245736134	13.601812833673787
33	33.493031874990315	28.436731758406737	22.092269660832017	15.977966705770932
34	19.416863327399327	26.05866369146096	25.980728615481098	28.543744365658615
35	20.12657385556215	23.984679149040122	35.63866199603123	20.250084999366493
36	36.895376490223484	26.469850975373426	24.464686295367557	12.170086239035538
37	15.4099065964066	42.37763053669466	31.544654975493746	10.667807891404992
38	16.696245534684756	39.84597112118038	26.15738145436878	17.300401889766075
39	20.102418539615176	38.311424917530104	28.352051424388847	13.234105118465871
40	26.65862704830243	24.242001627977142	26.287182095532906	22.81218922818752
41	13.127639424027885	23.596279899039896	31.21905954695566	32.05702112997656
42	15.277553695444308	20.620527278643873	29.42062791060163	34.68129111531019
43	16.455421592300194	21.404071036682353	30.927828473557224	31.21267889746023
44	11.189198107317056	30.82318582183221	34.70754293037709	23.280073140473647
45	11.350901995958314	51.51134798512762	21.5011480611485	15.636601957765569
46	18.287488366708686	40.87580794974236	24.89383055000287	15.942873133546081
47	17.368127926553257	29.16667806068362	28.323156197388126	25.142037815374994
48	18.193054754176362	26.626723800825292	39.596578878044824	15.583642566953523
49	23.915221221675615	26.68916301374483	37.0910801254618	12.304535639117757
50	19.38341049361616	41.40840987833924	26.15236808690809	13.055811541136503
51	16.32944934083333	36.03763489376674	25.961677819130465	21.67123794626946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1398.0
1	2306.5
2	3215.0
3	7906.5
4	12598.0
5	9463.5
6	6329.0
7	6585.5
8	6842.0
9	7185.5
10	7529.0
11	7465.5
12	7402.0
13	7258.5
14	7115.0
15	7039.0
16	6963.0
17	6598.5
18	6234.0
19	5602.5
20	4971.0
21	4491.0
22	4011.0
23	5061.5
24	6112.0
25	6996.0
26	9360.0
27	10840.0
28	14670.0
29	18500.0
30	23393.5
31	28287.0
32	33870.0
33	39453.0
34	43603.0
35	47753.0
36	47469.5
37	47186.0
38	56006.5
39	64827.0
40	93237.0
41	121647.0
42	138088.0
43	154529.0
44	144943.5
45	135358.0
46	120363.5
47	105369.0
48	89573.0
49	73777.0
50	60748.0
51	47719.0
52	39088.0
53	30457.0
54	25943.5
55	21430.0
56	19570.0
57	17710.0
58	14842.0
59	11974.0
60	10520.5
61	9067.0
62	7876.5
63	6686.0
64	5811.0
65	4936.0
66	4288.5
67	3641.0
68	3214.0
69	2787.0
70	2321.0
71	1855.0
72	1509.0
73	1163.0
74	939.0
75	557.5
76	400.0
77	308.5
78	217.0
79	156.0
80	95.0
81	68.0
82	41.0
83	35.0
84	29.0
85	19.5
86	10.0
87	7.5
88	5.0
89	3.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1097067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.449936065616235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59873877699826	13.71542966496691
2	6.974783663507232	2.4341905799941155
3	2.8448025002862876	1.4892486524790276
4	1.7775851721819977	1.2407499042305312
5	1.2308138551796115	1.073881154077943
6	0.947662108344937	0.9921985921455742
7	0.7677044267286681	0.9377475214594073
8	0.6416714409866784	0.89577004962795
9	0.5522139523344423	0.867248834649954
>10	3.6146607059435807	11.974118133122635
>50	0.6291215341545255	7.95173440954141
>100	1.3803974995240327	51.3581647852503
>500	0.03774729204927093	4.132076854033671
>1k	0.0015728038353862888	0.30500906040578873
>5k	5.242679451287628E-4	0.6324318040147623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6913	0.6301347137412756	No Hit
TTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1166	0.10628339016668992	No Hit
GGCCTGGGCAGTGTAGTCTTAGCAACTGGTGTATATTTTTATATGGTGTTT	1117	0.10181693551989075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019050796350633097	0.0
2	0.0	0.0	0.0	0.07283055638352079	0.0
3	0.0	0.0	0.0	0.10218154406248661	0.0
4	0.0	0.0	0.0	0.13891585472901838	0.0
5	0.0	0.0	0.0	0.21849166915056237	0.0
6	0.0	0.0	0.0	0.2979763314364574	0.0
7	0.0	0.0	0.0	0.3469250282799501	0.0
8	0.0	0.0	0.0	0.47216806266162414	0.0
9	0.0	0.0	0.0	0.5320550157829923	0.0
10	0.0	0.0	0.0	0.624209824924093	0.0
11	0.0	0.0	0.0	0.7251152390874942	0.0
12	0.0	0.0	0.0	0.8054202705942299	0.0
13	0.0	0.0	0.0	0.8379615830209094	0.0
14	0.0	0.0	0.0	0.8555539452011591	0.0
15	0.0	0.0	0.0	0.8787977397916444	0.0
16	0.0	0.0	0.0	0.9297517836194144	0.0
17	0.0	0.0	0.0	0.9938317349806347	0.0
18	0.0	0.0	0.0	1.08471041422265	0.0
19	0.0	0.0	0.0	1.1231766154665122	0.0
20	0.0	0.0	0.0	1.173766050751686	0.0
21	0.0	0.0	0.0	1.241583239674514	0.0
22	0.0	0.0	0.0	1.3043870611366488	0.0
23	0.0	0.0	0.0	1.383507114879948	0.0
24	0.0	0.0	0.0	1.4450348064429976	0.0
25	0.0	0.0	0.0	1.4953507853212247	0.0
26	0.0	0.0	0.0	1.5565138683416784	0.0
27	0.0	0.0	0.0	1.6172211906838871	0.0
28	0.0	0.0	0.0	1.677655056619149	0.0
29	0.0	0.0	0.0	1.7420996165229654	0.0
30	0.0	0.0	0.0	1.8232250172505418	0.0
31	0.0	0.0	0.0	1.8985166812965844	0.0
32	0.0	0.0	0.0	1.9605912856735277	0.0
33	0.0	0.0	0.0	2.0399847958237736	0.0
34	0.0	0.0	0.0	2.1340537998135027	0.0
35	0.0	0.0	0.0	2.246808991611269	0.0
36	0.0	0.0	0.0	2.3415160605505405	0.0
37	0.0	0.0	0.0	2.4292044150448424	0.0
38	0.0	0.0	0.0	2.5231822668989223	0.0
39	0.0	0.0	0.0	2.631926764728134	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.033145E-4	45.000004	21
CAGCGAA	20	7.033145E-4	45.000004	33
CTCACGA	40	6.8139343E-9	45.000004	24
AGTCCGT	20	7.033145E-4	45.000004	32
CGTATTC	75	0.0	45.000004	29
CGTGCCT	20	7.033145E-4	45.000004	33
GCGCGAT	20	7.033145E-4	45.000004	3
CGGTTTA	20	7.033145E-4	45.000004	17
GACCGTT	40	6.8139343E-9	45.000004	9
GGCGTAA	30	2.1654105E-6	45.000004	34
GCACGAG	30	2.1654105E-6	45.000004	1
CGCGATA	20	7.033145E-4	45.000004	4
TAAGTCG	40	6.8139343E-9	45.000004	1
TACGGGC	40	6.8139343E-9	45.000004	4
ACTTACG	20	7.033145E-4	45.000004	43
ATCGTAG	60	0.0	45.000004	1
CCGTACG	20	7.033145E-4	45.000004	35
CACTCGA	20	7.033145E-4	45.000004	10
TGCGCGA	20	7.033145E-4	45.000004	2
CGTAGTA	20	7.033145E-4	45.000004	18
>>END_MODULE