FastQCFastQC Report
Thu 26 May 2016
SRR1545148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545148_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1228098
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43490.3541248336859111No Hit
GTATTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC13310.10837897301355429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGC1450.045.0000044
ACGGGTA950.045.0000045
TACGTGG1250.045.0000042
TCACGAC502.1827873E-1145.025
TATCACG207.033475E-445.01
CGGGTAT1400.045.06
ATCTCGC502.1827873E-1145.011
TCGTTAG800.045.01
ATCTCCG253.890963E-545.013
ACCGGGC207.033475E-445.04
TCCGCAA207.033475E-445.032
GATCGCG351.2120108E-745.010
CACGGGT351.2120108E-745.04
TAGACCG207.033475E-445.02
ACGCACG207.033475E-445.01
GACACGG406.8157533E-945.02
GACACGC207.033475E-445.013
ATGTGCG207.033475E-445.01
GATCCTA207.033475E-445.023
GATCCGG406.8157533E-945.02