##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545148_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1228098
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.852462099930136	31.0	31.0	33.0	30.0	33.0
2	31.152644984357927	31.0	31.0	33.0	30.0	34.0
3	31.313308872744685	31.0	31.0	34.0	28.0	34.0
4	35.149745378626136	35.0	35.0	37.0	32.0	37.0
5	35.04137454828523	37.0	35.0	37.0	32.0	37.0
6	35.120427685738434	37.0	35.0	37.0	32.0	37.0
7	35.53314230623289	36.0	35.0	37.0	35.0	37.0
8	35.60682942240766	37.0	35.0	37.0	35.0	37.0
9	37.458980472242445	39.0	37.0	39.0	35.0	39.0
10	36.94098597994623	39.0	37.0	39.0	33.0	39.0
11	36.51897894141999	39.0	35.0	39.0	32.0	39.0
12	34.87453444269106	35.0	34.0	39.0	30.0	39.0
13	34.323620753392646	35.0	33.0	39.0	27.0	39.0
14	34.91805539948766	35.0	33.0	40.0	27.0	41.0
15	35.44463389729484	35.0	33.0	40.0	30.0	41.0
16	35.71365314494446	35.0	34.0	40.0	31.0	41.0
17	35.574784748448415	35.0	34.0	40.0	31.0	41.0
18	35.68984397010662	36.0	35.0	39.0	31.0	41.0
19	35.53090144271874	36.0	34.0	39.0	31.0	41.0
20	35.20489732904051	35.0	34.0	39.0	30.0	41.0
21	35.03584730208827	35.0	34.0	39.0	29.0	41.0
22	34.87007225807712	35.0	34.0	39.0	29.0	41.0
23	34.85660102043974	35.0	34.0	39.0	29.0	41.0
24	34.98789103149748	35.0	34.0	39.0	30.0	41.0
25	34.91372594043798	35.0	34.0	39.0	30.0	41.0
26	34.6510563489233	35.0	34.0	38.0	29.0	41.0
27	34.65577339919127	35.0	34.0	39.0	29.0	41.0
28	34.76894759213027	35.0	34.0	39.0	29.0	41.0
29	34.75260931945171	35.0	34.0	38.0	29.0	41.0
30	34.729746323176165	35.0	34.0	38.0	29.0	41.0
31	34.4428506519838	35.0	34.0	38.0	29.0	41.0
32	34.27492349959042	35.0	34.0	39.0	27.0	41.0
33	34.00605814845395	35.0	33.0	39.0	27.0	41.0
34	33.81466625627596	35.0	33.0	38.0	24.0	41.0
35	33.553983476888654	35.0	33.0	38.0	23.0	41.0
36	33.24631666202534	35.0	33.0	38.0	23.0	41.0
37	33.25812598017422	35.0	32.0	38.0	23.0	41.0
38	33.351147058296654	35.0	32.0	38.0	23.0	41.0
39	33.39336030186516	35.0	33.0	38.0	23.0	41.0
40	32.993526575240736	35.0	32.0	38.0	23.0	41.0
41	33.14570498445564	35.0	32.0	38.0	23.0	41.0
42	33.1503471221352	35.0	32.0	38.0	23.0	41.0
43	33.12924620022181	35.0	32.0	38.0	23.0	41.0
44	33.08649554025819	35.0	32.0	38.0	23.0	41.0
45	33.14831552530824	35.0	32.0	38.0	23.0	41.0
46	33.163803702961815	35.0	33.0	38.0	23.0	40.0
47	33.1063213196341	35.0	32.0	38.0	23.0	40.0
48	33.16198300135657	35.0	33.0	38.0	23.0	40.0
49	33.2375795742685	35.0	33.0	38.0	24.0	40.0
50	32.99557201461121	35.0	32.0	38.0	23.0	40.0
51	32.68281032946882	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	4.0
14	6.0
15	27.0
16	105.0
17	321.0
18	856.0
19	1683.0
20	2837.0
21	4677.0
22	7105.0
23	10344.0
24	14486.0
25	19907.0
26	25260.0
27	28996.0
28	31777.0
29	35906.0
30	42795.0
31	52409.0
32	66228.0
33	89072.0
34	169641.0
35	242254.0
36	59964.0
37	73727.0
38	104900.0
39	142744.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.92921086102249	23.454561443793573	31.348068313766493	13.268159381417444
2	29.697711420424106	26.539494405169624	31.613926575892155	12.148867598514125
3	28.195144035736565	26.043687067318732	33.82246367960863	11.938705217336077
4	24.137324545761004	29.975620838076438	32.5098648479193	13.37718976824325
5	22.223552192088906	32.77393172206126	33.07382635587714	11.92868972997269
6	19.105885686647156	42.08426363368395	29.794202091364042	9.015648588304842
7	87.61222638584218	4.4468763893435215	6.943256971349192	0.9976402534651143
8	90.42845114966394	2.5985711238028233	6.078505135583642	0.8944725909495821
9	87.14190561339568	3.8839734288305983	7.739773210281264	1.234347747492464
10	65.01728689404266	18.248055122636792	11.386469157998793	5.348188825321758
11	61.876413771539404	13.682947126369394	17.204164488501732	7.236474613589469
12	55.65182908855808	19.491441236774264	18.038462728544467	6.818266946123192
13	16.777732721655763	57.449568356922654	17.642484557421316	8.130214364000267
14	9.038529498460221	56.485231634608965	24.614810870142286	9.86142799678853
15	7.498912953200803	21.257749788697645	62.491836970665204	8.751500287436345
16	7.696454191766455	14.460572364746135	60.9061328981889	16.9368405452985
17	7.991056088357769	15.173219075350664	30.30344483909265	46.53227999719892
18	14.739214622937258	20.839134987598708	47.76988481375265	16.651765575711387
19	34.109248610452916	17.590127172261496	28.833610998470803	19.467013218814785
20	34.77393497913033	18.219881475256862	30.122270372559846	16.883913173052964
21	15.259368552021094	34.60554450866299	32.26705034940208	17.868036589913835
22	19.07991056088358	22.947110084048667	20.462047817030886	37.51093153803687
23	10.96500442147125	34.42347434813834	24.680277958273688	29.93124327211672
24	9.058153339554336	18.60755412027379	60.33565725210855	11.99863528806333
25	8.591333916348695	23.261905808819815	51.78397815158073	16.36278212325075
26	8.524482573866255	41.727044584389844	30.418093670049135	19.330379171694766
27	8.087953892930368	54.56649225061844	25.37126515962081	11.974288696830383
28	7.470413598914745	35.88801545153563	44.837464111170284	11.804106838379349
29	8.536208022486806	23.806569182589662	42.57257971269394	25.084643082229597
30	12.694833799908475	45.30591206890655	29.650728199215372	12.348525931969599
31	33.536411589303135	31.46882414921285	25.264759001317483	9.730005260166534
32	37.43772891088496	20.800538719222732	30.709031363946526	11.05270100594578
33	38.0437066097331	27.803888614752243	19.06378806903032	15.088616706484338
34	19.57856783416307	25.11037392781358	23.77929122920158	31.53176700882177
35	18.00605489138489	23.904769814786768	36.692348656214726	21.39682663761361
36	41.87247271797527	25.213378736876045	21.380215585401167	11.533932959747512
37	14.828295461762824	46.94462494035492	28.286260542725415	9.940819055156835
38	15.48882906738713	43.6695605725276	22.662116541188084	18.17949381889719
39	19.262387855040885	41.34132618080967	26.615221260844002	12.781064703305436
40	29.231950544663377	22.11957026230806	25.41433989795603	23.23413929507254
41	11.89245483666613	22.936768889779156	29.023335271289426	36.14744100226529
42	15.319135769295283	19.00198518359284	27.051505661600295	38.62737338551158
43	16.35553514458944	19.413027299124337	30.393584225363124	33.8378533309231
44	10.858172556261797	30.226496582520284	36.04207481813341	22.87325604308451
45	10.009787492529098	56.845789179690875	20.78303197301844	12.36139135476159
46	18.777735978724824	44.25501873628978	22.886121465876503	14.081123819108898
47	17.158565521644036	30.170637848119615	24.629549107644504	28.041247522591846
48	17.658362769094975	26.85714006536938	41.1089343032885	14.375562862247149
49	23.408392489850158	26.049956925261665	39.08548014897834	11.456170435909838
50	19.525314755011408	46.01986160713559	23.014368560163764	11.44045507768924
51	15.94188737380893	40.38863348039	23.849399640745283	19.820079505055784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1493.0
1	2190.5
2	2888.0
3	6222.0
4	9556.0
5	7792.0
6	6028.0
7	6213.0
8	6398.0
9	6777.0
10	7156.0
11	7165.5
12	7175.0
13	6930.5
14	6686.0
15	6430.5
16	6175.0
17	5704.5
18	5234.0
19	5016.0
20	4798.0
21	5117.5
22	5437.0
23	6154.5
24	6872.0
25	7332.0
26	9109.5
27	10427.0
28	14757.5
29	19088.0
30	21889.5
31	24691.0
32	30544.5
33	36398.0
34	39569.5
35	42741.0
36	46841.0
37	50941.0
38	59936.5
39	68932.0
40	112733.5
41	156535.0
42	179171.5
43	201808.0
44	191805.5
45	181803.0
46	154941.5
47	128080.0
48	104903.5
49	81727.0
50	68468.0
51	55209.0
52	42583.0
53	29957.0
54	22205.5
55	14454.0
56	12280.0
57	10106.0
58	9151.0
59	8196.0
60	7540.5
61	6885.0
62	5927.0
63	4969.0
64	4370.0
65	3771.0
66	3298.5
67	2826.0
68	2329.0
69	1832.0
70	1538.0
71	1244.0
72	998.0
73	752.0
74	633.0
75	396.5
76	279.0
77	218.5
78	158.0
79	101.0
80	44.0
81	32.5
82	21.0
83	17.0
84	13.0
85	8.5
86	4.0
87	3.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1228098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.980993379588664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59151411423213	13.685239676248024
2	7.406291205734071	2.51532363863752
3	2.6762592868425608	1.3633662369580861
4	1.460094235612014	0.9917540219401272
5	0.993313005087119	0.8433720781621846
6	0.6769582381906181	0.6897254016583735
7	0.5442516613837322	0.6469353701151103
8	0.4465967490061072	0.6066925150574821
9	0.3915891998015322	0.5984616248413417
>10	3.036926664815327	10.370655989331128
>50	0.43960247882908277	5.384029173803537
>100	1.2817730919903205	55.20455288482119
>500	0.05002041334596373	5.90175255799478
>1k	0.004809655129419589	1.1981388304311236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4349	0.3541248336859111	No Hit
GTATTTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	1331	0.10837897301355429	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.142672653159601E-5	0.0	0.0	0.02410231105335242	0.0
2	8.142672653159601E-5	0.0	0.0	0.07996104545402728	0.0
3	8.142672653159601E-5	0.0	0.0	0.10528475740535365	0.0
4	8.142672653159601E-5	0.0	0.0	0.15658359512025913	0.0
5	8.142672653159601E-5	0.0	0.0	0.265451128493003	0.0
6	8.142672653159601E-5	0.0	0.0	0.371468726437141	0.0
7	8.142672653159601E-5	0.0	0.0	0.4431242457849455	0.0
8	8.142672653159601E-5	0.0	0.0	0.6353727471260436	0.0
9	8.142672653159601E-5	0.0	0.0	0.7058882923024058	0.0
10	8.142672653159601E-5	0.0	0.0	0.8340539598631379	0.0
11	8.142672653159601E-5	0.0	0.0	0.9371401956521385	0.0
12	8.142672653159601E-5	0.0	0.0	1.0451120350330347	0.0
13	8.142672653159601E-5	0.0	0.0	1.086395385384554	0.0
14	8.142672653159601E-5	0.0	0.0	1.1006450625275832	0.0
15	8.142672653159601E-5	0.0	0.0	1.126701615017694	0.0
16	8.142672653159601E-5	0.0	0.0	1.188993060814365	0.0
17	8.142672653159601E-5	0.0	0.0	1.2669998648316338	0.0
18	8.142672653159601E-5	0.0	0.0	1.395409812571961	0.0
19	8.142672653159601E-5	0.0	0.0	1.4436958614051973	0.0
20	8.142672653159601E-5	0.0	0.0	1.4901905222547387	0.0
21	8.142672653159601E-5	0.0	0.0	1.562497455414796	0.0
22	8.142672653159601E-5	0.0	0.0	1.6243003408522774	0.0
23	8.142672653159601E-5	0.0	0.0	1.7130554727717169	0.0
24	8.142672653159601E-5	0.0	0.0	1.7781968539969937	0.0
25	8.142672653159601E-5	0.0	0.0	1.82851857099352	0.0
26	8.142672653159601E-5	0.0	0.0	1.8816087966921207	0.0
27	8.142672653159601E-5	0.0	0.0	1.9345361689376581	0.0
28	8.142672653159601E-5	0.0	0.0	1.9876263946362587	0.0
29	8.142672653159601E-5	0.0	0.0	2.0527677758615357	0.0
30	8.142672653159601E-5	0.0	0.0	2.147629912270845	0.0
31	8.142672653159601E-5	0.0	0.0	2.234430802753526	0.0
32	8.142672653159601E-5	0.0	0.0	2.307796283358494	0.0
33	8.142672653159601E-5	0.0	0.0	2.3861287942818894	0.0
34	8.142672653159601E-5	0.0	0.0	2.4730925382176343	0.0
35	8.142672653159601E-5	0.0	0.0	2.6060623826437306	0.0
36	8.142672653159601E-5	0.0	0.0	2.696446049093802	0.0
37	8.142672653159601E-5	0.0	0.0	2.7916338924092376	0.0
38	8.142672653159601E-5	0.0	0.0	2.882668972671562	0.0
39	8.142672653159601E-5	0.0	0.0	2.9703655571460907	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGGC	145	0.0	45.000004	4
ACGGGTA	95	0.0	45.000004	5
TACGTGG	125	0.0	45.000004	2
TCACGAC	50	2.1827873E-11	45.0	25
TATCACG	20	7.033475E-4	45.0	1
CGGGTAT	140	0.0	45.0	6
ATCTCGC	50	2.1827873E-11	45.0	11
TCGTTAG	80	0.0	45.0	1
ATCTCCG	25	3.890963E-5	45.0	13
ACCGGGC	20	7.033475E-4	45.0	4
TCCGCAA	20	7.033475E-4	45.0	32
GATCGCG	35	1.2120108E-7	45.0	10
CACGGGT	35	1.2120108E-7	45.0	4
TAGACCG	20	7.033475E-4	45.0	2
ACGCACG	20	7.033475E-4	45.0	1
GACACGG	40	6.8157533E-9	45.0	2
GACACGC	20	7.033475E-4	45.0	13
ATGTGCG	20	7.033475E-4	45.0	1
GATCCTA	20	7.033475E-4	45.0	23
GATCCGG	40	6.8157533E-9	45.0	2
>>END_MODULE