FastQCFastQC Report
Thu 26 May 2016
SRR1545146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545146_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1559603
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51640.33110990425127423No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29180.18709889632169213No Hit
TTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA21580.1383685463544248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19460.12477534346881866No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16330.10470613354808884No Hit
GATAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA15700.10066664401132852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGC650.045.0000041
TAAACGG650.045.0000042
AGTTCCC650.045.00000417
TTGTTCG1150.045.0000041
TGATCCG351.2123019E-745.01
TCACGAC700.045.025
CGGGTTC207.034063E-445.06
TATCACT207.034063E-445.045
TGTCACG551.8189894E-1245.02
ACTATCC406.8175723E-945.044
CTCACGA700.045.024
ACACGGC253.8914488E-545.03
GCGACTT253.8914488E-545.041
GTCGATA502.1827873E-1145.09
CAACGCG207.034063E-445.040
TTAGCGT551.8189894E-1245.030
TTAGCGA253.8914488E-545.031
CGTTGGC253.8914488E-545.015
TGAACCG406.8175723E-945.01
AGGCGTA207.034063E-445.012