##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545146_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1559603
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.824073177597118	31.0	31.0	33.0	28.0	33.0
2	31.14329159407875	31.0	31.0	33.0	30.0	34.0
3	31.31039629957111	31.0	31.0	34.0	28.0	34.0
4	35.15093264119138	35.0	35.0	37.0	32.0	37.0
5	35.009117063765586	35.0	35.0	37.0	32.0	37.0
6	35.08718436679078	37.0	35.0	37.0	32.0	37.0
7	35.50919112107376	36.0	35.0	37.0	35.0	37.0
8	35.58492962632157	37.0	35.0	37.0	35.0	37.0
9	37.4157622164102	39.0	37.0	39.0	35.0	39.0
10	36.954628838236395	39.0	37.0	39.0	32.0	39.0
11	36.52281830696658	39.0	35.0	39.0	32.0	39.0
12	34.968648431684215	35.0	34.0	39.0	30.0	39.0
13	34.40282623206034	35.0	33.0	39.0	27.0	39.0
14	34.980141100010705	35.0	33.0	40.0	27.0	41.0
15	35.48246573006079	35.0	33.0	40.0	30.0	41.0
16	35.728445636485695	35.0	34.0	40.0	31.0	41.0
17	35.60337855210589	35.0	34.0	40.0	31.0	41.0
18	35.73204655287275	36.0	35.0	39.0	31.0	41.0
19	35.56501366052771	36.0	34.0	39.0	30.0	41.0
20	35.28024952503939	35.0	34.0	39.0	30.0	41.0
21	35.109714459384854	35.0	34.0	39.0	29.0	41.0
22	34.921365244873215	35.0	34.0	39.0	29.0	41.0
23	34.89522718281511	35.0	34.0	39.0	29.0	41.0
24	35.010344299158184	35.0	34.0	39.0	30.0	41.0
25	34.935481657832156	35.0	34.0	39.0	30.0	41.0
26	34.679202335466144	35.0	34.0	39.0	29.0	41.0
27	34.673535508716	35.0	34.0	39.0	29.0	41.0
28	34.76717536449981	35.0	34.0	39.0	29.0	41.0
29	34.774316284336464	35.0	34.0	39.0	29.0	41.0
30	34.74870335591814	35.0	34.0	39.0	29.0	41.0
31	34.468496790529386	35.0	34.0	39.0	29.0	41.0
32	34.32288922244956	35.0	34.0	39.0	27.0	41.0
33	34.078964967366694	35.0	33.0	39.0	27.0	41.0
34	33.86697768598804	35.0	33.0	39.0	25.0	41.0
35	33.64916969254355	35.0	33.0	39.0	23.0	41.0
36	33.363397608237484	35.0	33.0	38.0	23.0	41.0
37	33.37424331704928	35.0	33.0	38.0	23.0	41.0
38	33.449736246980805	35.0	33.0	38.0	23.0	41.0
39	33.48356344531268	35.0	33.0	38.0	23.0	41.0
40	33.10605840075968	35.0	32.0	38.0	23.0	41.0
41	33.246581982722525	35.0	32.0	38.0	23.0	41.0
42	33.24626908258063	35.0	32.0	38.0	23.0	41.0
43	33.19518492847218	35.0	32.0	38.0	23.0	41.0
44	33.18358197566945	35.0	32.0	38.0	23.0	41.0
45	33.2603867779172	35.0	33.0	38.0	23.0	41.0
46	33.27274633352206	35.0	33.0	38.0	23.0	41.0
47	33.22033684213226	35.0	33.0	38.0	23.0	41.0
48	33.265356632425046	35.0	33.0	38.0	23.0	41.0
49	33.3257591835871	35.0	33.0	38.0	24.0	40.0
50	33.055133902666256	35.0	32.0	38.0	23.0	40.0
51	32.73047948740801	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	1.0
12	0.0
13	7.0
14	12.0
15	33.0
16	136.0
17	437.0
18	1013.0
19	2082.0
20	3599.0
21	5877.0
22	9043.0
23	12912.0
24	18202.0
25	24755.0
26	31447.0
27	36196.0
28	40259.0
29	45936.0
30	54269.0
31	66135.0
32	84477.0
33	112662.0
34	207329.0
35	302912.0
36	81312.0
37	96172.0
38	134801.0
39	187496.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.78046143794286	25.61562141134635	31.21973989534516	12.384177255365628
2	29.241992994371003	26.35350150006123	32.78064994745458	11.623855558113187
3	27.550985731625293	26.26322211485872	34.4627446856668	11.72304746784919
4	24.724689552405323	29.78879881610897	32.50763174987481	12.978879881610897
5	21.436480950600888	32.92017263367665	32.82489197571433	12.818454440008132
6	19.093769375924513	43.18701618296451	28.030787322158268	9.688427118952708
7	85.68885799783664	5.784036065588486	7.063207752229253	1.4638981843456316
8	89.0670253904359	3.2719865247758566	6.346486894421209	1.3145011903670356
9	85.32408568077902	5.14246253694049	7.724016945338012	1.8094348369424782
10	64.06495755650636	18.499259106323855	11.644758313493883	5.791025023675897
11	60.80021646534407	14.820822991492067	17.24785089538812	7.131109647775748
12	55.33267119901667	19.932636703058407	17.571651247144302	7.163040850780615
13	18.59050027474941	55.5148329414601	18.024009956379924	7.870656827410566
14	9.259343563714612	56.53066838163302	24.167175877450862	10.042812177201506
15	8.167014297869393	22.660125685831588	60.172813209515496	9.000046806783521
16	8.82288633709989	15.83249070436515	58.955067411386096	16.389555547148856
17	8.946379302937991	16.037607006398424	30.92799898435692	44.088014706306666
18	14.789917690591773	21.418207069363167	46.647255743929705	17.144619496115357
19	33.58335422540223	18.49015422514576	28.529568101625863	19.396923447826143
20	34.16920844599555	19.724570932474485	28.94435314628146	17.16186747524851
21	16.573640856038363	34.452421545739526	31.419213735803282	17.554723862418832
22	19.248103523781374	25.416019333125163	19.917568765897474	35.41830837719599
23	12.067558218341462	34.417540874184006	24.468149907380276	29.046751000094257
24	9.796275077696055	20.88396854840623	56.839272558465204	12.480483815432516
25	8.961126645691243	24.743348146932263	50.63878435730118	15.656740850075307
26	8.669321615821463	42.309356932501416	30.219485343385465	18.80183610829166
27	8.990108380145461	53.68782953097679	25.272393038484793	12.049669050392954
28	7.826286561387738	37.779999140806986	42.69407022171668	11.699644076088594
29	8.414449061716347	25.403836745633345	41.83987848189572	24.341835710754594
30	13.45329548609486	44.71612326983213	29.248981952458415	12.581599291614598
31	32.795653765733974	31.434474029608815	24.963788861652613	10.806083343004598
32	37.805390217895194	21.46078200670299	29.815023438657146	10.918804336744671
33	38.04224536628873	27.038291154864414	19.509836798210827	15.409626680636034
34	20.03727871772496	26.688971488256946	23.391145054222132	29.88260473979596
35	18.06139126431534	23.52765415301202	35.37124511814866	23.039709464523987
36	40.86713093011491	25.056697120998102	22.04375087762719	12.032421071259801
37	15.936940362387094	45.19156477642067	28.225772840908874	10.645722020283367
38	15.513563387605691	44.27639598025908	21.945520751114227	18.264519881021005
39	19.57870047698036	40.7956383772024	26.570287438534034	13.055373707283199
40	28.165629330028217	24.35421065489102	24.278550374678684	23.201609640402076
41	13.48811203876884	22.97148697456981	28.00834571362071	35.53205527304064
42	15.899943767740893	19.591652491050606	26.627737956390185	37.88066578481832
43	15.640775248572874	20.14269015896994	30.30848235095726	33.90805224149992
44	10.606160670375731	30.954993033483518	34.99865029754367	23.44019599859708
45	9.969267820079853	54.560423389798565	21.0570895285531	14.41321926156849
46	18.131537320715594	44.05133870606815	23.00386700974543	14.813256963470833
47	17.476370589181993	30.838745501258973	23.95769949147315	27.727184418085887
48	17.710532744551017	27.546882123206995	39.50492529188518	15.237659840356809
49	23.301827452242655	26.027328749688223	38.39066736855469	12.280176429514436
50	19.03240760629468	45.11494271298529	23.647428223721036	12.205221456998991
51	16.08601676195801	40.23158457633129	23.152045744974842	20.53035291673586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2385.0
1	2923.0
2	3461.0
3	6894.0
4	10327.0
5	8454.0
6	6581.0
7	7089.0
8	7597.0
9	8161.0
10	8725.0
11	8691.0
12	8657.0
13	8507.5
14	8358.0
15	7858.0
16	7358.0
17	6675.5
18	5993.0
19	5849.0
20	5705.0
21	5841.0
22	5977.0
23	6353.0
24	6729.0
25	8048.5
26	13027.0
27	16686.0
28	21685.5
29	26685.0
30	31129.0
31	35573.0
32	40486.5
33	45400.0
34	50026.0
35	54652.0
36	57639.5
37	60627.0
38	76485.0
39	92343.0
40	137918.5
41	183494.0
42	221623.5
43	259753.0
44	247759.0
45	235765.0
46	202085.5
47	168406.0
48	139036.5
49	109667.0
50	91126.0
51	72585.0
52	55556.0
53	38527.0
54	28996.5
55	19466.0
56	15354.5
57	11243.0
58	9718.5
59	8194.0
60	7772.5
61	7351.0
62	6344.0
63	5337.0
64	4364.0
65	3391.0
66	2916.5
67	2442.0
68	1996.0
69	1550.0
70	1476.0
71	1402.0
72	1119.0
73	836.0
74	673.5
75	400.0
76	289.0
77	193.5
78	98.0
79	80.5
80	63.0
81	43.5
82	24.0
83	16.5
84	9.0
85	7.0
86	5.0
87	5.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1559603.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.270569754111655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.60092104071906	13.920238290793844
2	7.887175574579428	2.724320318473993
3	2.828416948646001	1.465451166159072
4	1.427521261346647	0.9861642207825885
5	0.899837213757935	0.7770350683775947
6	0.667279186882375	0.6914575045511377
7	0.48239907976107665	0.5831914869433061
8	0.3981849514974576	0.5501504783899525
9	0.32457179558112736	0.5044985852221016
>10	2.82567760860354	9.845962704918849
>50	0.44459705608044053	5.50131904587508
>100	1.129567203437863	51.542378997875296
>500	0.0737889496142187	8.319816613046664
>1k	0.009689458030149931	2.2556476464691397
>5k	3.7267146269807423E-4	0.33236787212140145
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5164	0.33110990425127423	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2918	0.18709889632169213	No Hit
TTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2158	0.1383685463544248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1946	0.12477534346881866	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1633	0.10470613354808884	No Hit
GATAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1570	0.10066664401132852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02872525892807336	0.0
2	0.0	0.0	0.0	0.10682205663877281	0.0
3	0.0	0.0	0.0	0.15439826673839432	0.0
4	0.0	0.0	0.0	0.2432029176655854	0.0
5	0.0	0.0	0.0	0.42491582793826377	0.0
6	0.0	0.0	0.0	0.600922157754249	0.0
7	0.0	0.0	0.0	0.7273004732614646	0.0
8	0.0	0.0	0.0	1.0770689720396793	0.0
9	0.0	0.0	0.0	1.2167198960248218	0.0
10	0.0	0.0	0.0	1.4593457437565842	0.0
11	0.0	0.0	0.0	1.6328514371926701	0.0
12	0.0	0.0	0.0	1.8017405711581729	0.0
13	0.0	0.0	0.0	1.8723995786107106	0.0
14	0.0	0.0	0.0	1.893174096228335	0.0
15	0.0	0.0	0.0	1.9358772713312298	0.0
16	0.0	0.0	0.0	2.030388502715114	0.0
17	0.0	0.0	0.0	2.13778762928771	0.0
18	0.0	0.0	0.0	2.3302083927768797	0.0
19	0.0	0.0	0.0	2.3986232393756617	0.0
20	0.0	0.0	0.0	2.472616428668065	0.0
21	0.0	0.0	0.0	2.5753348768885416	0.0
22	0.0	0.0	0.0	2.681900458001171	0.0
23	0.0	0.0	0.0	2.8082787735083863	0.0
24	0.0	0.0	0.0	2.9112536972550065	0.0
25	0.0	0.0	0.0	2.9896069704918493	0.0
26	0.0	0.0	0.0	3.0741797752376727	0.0
27	0.0	0.0	0.0	3.1577907967604575	0.0
28	0.0	0.0	0.0	3.247557230910687	0.0
29	0.0	0.0	0.0	3.3466850217651545	0.0
30	0.0	0.0	0.0	3.491465456273167	0.0
31	0.0	0.0	0.0	3.6247686109862576	0.0
32	0.0	0.0	0.0	3.736591940384829	0.0
33	0.0	0.0	0.0	3.84732524879729	0.0
34	0.0	0.0	0.0	3.973062375489147	0.0
35	0.0	0.0	0.0	4.145606285702195	0.0
36	0.0	0.0	0.0	4.278075894955319	0.0
37	0.0	0.0	0.0	4.414841469271346	0.0
38	0.0	0.0	0.0	4.54327158898771	0.0
39	0.0	0.0	0.0	4.676061792648514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGC	65	0.0	45.000004	1
TAAACGG	65	0.0	45.000004	2
AGTTCCC	65	0.0	45.000004	17
TTGTTCG	115	0.0	45.000004	1
TGATCCG	35	1.2123019E-7	45.0	1
TCACGAC	70	0.0	45.0	25
CGGGTTC	20	7.034063E-4	45.0	6
TATCACT	20	7.034063E-4	45.0	45
TGTCACG	55	1.8189894E-12	45.0	2
ACTATCC	40	6.8175723E-9	45.0	44
CTCACGA	70	0.0	45.0	24
ACACGGC	25	3.8914488E-5	45.0	3
GCGACTT	25	3.8914488E-5	45.0	41
GTCGATA	50	2.1827873E-11	45.0	9
CAACGCG	20	7.034063E-4	45.0	40
TTAGCGT	55	1.8189894E-12	45.0	30
TTAGCGA	25	3.8914488E-5	45.0	31
CGTTGGC	25	3.8914488E-5	45.0	15
TGAACCG	40	6.8175723E-9	45.0	1
AGGCGTA	20	7.034063E-4	45.0	12
>>END_MODULE