FastQCFastQC Report
Thu 26 May 2016
SRR1545145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545145_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1452356
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149261.0277094596641594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.033902E-445.01
TATGCGA207.033902E-445.012
ATCGTTA207.033902E-445.035
CGTTTTT108200.043.5651551
CGGTCTA2250.043.031
ACGTAGG2650.042.452832
TGCGTAG750.042.0000041
ACGGTCT2350.041.1702130
GACGTAG1100.040.9090921
TACGTTG556.184564E-1140.9090921
TCACGAC2550.040.58823425
TATACGG2400.040.3125042
CATACGA10350.040.21739218
CGTTCGA1850.040.13513614
CGTAAGG2750.040.0909082
ACGGGAC3200.040.0781255
AATACGG900.040.02
AAGGGAC11850.039.873425
ACATACG10500.039.8571417
TCGTAAG1300.039.807691
Produced by FastQC (version 0.11.5)