##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545145_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1452356
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.766875339104185	31.0	30.0	31.0	28.0	33.0
2	31.076006158269735	31.0	31.0	33.0	28.0	34.0
3	31.106446353373414	31.0	31.0	34.0	28.0	34.0
4	35.05782328850502	35.0	35.0	37.0	32.0	37.0
5	34.98923955283691	35.0	35.0	37.0	32.0	37.0
6	35.07939788867192	37.0	35.0	37.0	32.0	37.0
7	35.44344430704318	35.0	35.0	37.0	35.0	37.0
8	35.487964383388096	36.0	35.0	37.0	35.0	37.0
9	37.290273872246196	39.0	37.0	39.0	35.0	39.0
10	36.75237131942857	39.0	37.0	39.0	32.0	39.0
11	36.46200862598426	38.0	35.0	39.0	32.0	39.0
12	35.846305588987825	37.0	35.0	39.0	31.0	39.0
13	35.613130664933394	37.0	35.0	39.0	30.0	39.0
14	36.460084166691914	38.0	35.0	40.0	31.0	41.0
15	36.67237164992605	38.0	35.0	40.0	31.0	41.0
16	36.668601224493166	38.0	35.0	40.0	31.0	41.0
17	36.43100796223516	38.0	35.0	40.0	31.0	41.0
18	36.41301306291295	38.0	35.0	40.0	31.0	41.0
19	36.292095051075634	37.0	35.0	40.0	31.0	41.0
20	36.046251745439825	37.0	34.0	40.0	30.0	41.0
21	35.97276631900168	37.0	34.0	40.0	30.0	41.0
22	35.81410618333246	37.0	34.0	40.0	30.0	41.0
23	35.64714642966325	37.0	34.0	40.0	30.0	41.0
24	35.66834715455439	37.0	34.0	40.0	30.0	41.0
25	35.47967096221588	36.0	34.0	40.0	29.0	41.0
26	35.183526628457486	36.0	34.0	40.0	29.0	41.0
27	35.19507338421158	36.0	34.0	40.0	29.0	41.0
28	35.14301383407374	36.0	34.0	40.0	27.0	41.0
29	35.05985722508807	36.0	34.0	40.0	27.0	41.0
30	35.00697969368392	36.0	34.0	40.0	27.0	41.0
31	34.85997854520517	36.0	34.0	40.0	27.0	41.0
32	34.687467122385975	36.0	34.0	40.0	25.0	41.0
33	34.35468370013964	36.0	33.0	40.0	24.0	41.0
34	33.9682274869247	36.0	33.0	40.0	23.0	41.0
35	33.66935172919036	36.0	33.0	40.0	21.0	41.0
36	33.514790450826105	36.0	33.0	40.0	20.0	41.0
37	33.51689737226961	36.0	33.0	40.0	21.0	41.0
38	33.45640875928491	35.0	32.0	40.0	20.0	41.0
39	33.39840851692009	35.0	32.0	40.0	19.0	41.0
40	33.1846461886755	35.0	32.0	40.0	18.0	41.0
41	33.10612756101121	35.0	32.0	40.0	18.0	41.0
42	33.07210904213567	35.0	32.0	40.0	18.0	41.0
43	32.96680703629138	35.0	31.0	40.0	18.0	41.0
44	32.873774749441594	35.0	31.0	40.0	18.0	41.0
45	32.9059046129186	35.0	31.0	40.0	18.0	41.0
46	32.877677373867016	35.0	31.0	40.0	18.0	41.0
47	32.778599048718085	35.0	31.0	40.0	18.0	41.0
48	32.78237291683306	35.0	31.0	39.0	18.0	41.0
49	32.75157537132769	35.0	31.0	39.0	18.0	41.0
50	32.608324680725666	35.0	31.0	39.0	18.0	41.0
51	32.33768235887069	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	1.0
13	4.0
14	15.0
15	64.0
16	156.0
17	489.0
18	1150.0
19	2415.0
20	4090.0
21	6581.0
22	9797.0
23	14271.0
24	20029.0
25	27917.0
26	35152.0
27	39026.0
28	39790.0
29	42381.0
30	48256.0
31	57049.0
32	69054.0
33	87952.0
34	134752.0
35	178086.0
36	110547.0
37	135135.0
38	182306.0
39	205854.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.055286720335786	21.797341698591804	25.38420332205052	18.763168259021892
2	35.879426256372405	24.355392204115244	27.83952419379271	11.925657345719644
3	28.071560967145796	24.846318671179795	34.9713155727659	12.110804788908505
4	24.9924260993861	27.048120433282197	34.588145055344555	13.371308411987142
5	22.040877030149634	30.838926544180627	33.80162990341211	13.318566522257628
6	18.78981461845443	41.43908242882599	30.943033250800767	8.828069701918812
7	82.8723122980867	4.19387533084175	11.470879040675976	1.462933330395578
8	83.51099868076423	3.6209441762212573	11.342880120301082	1.5251770227134394
9	78.95963524094644	5.461126610830953	12.976708190003002	2.6025299582196104
10	42.39408244259672	26.955236870299014	19.345670069872675	11.305010617231588
11	35.245903896840716	22.479818997546055	27.62690414746798	14.64737295814525
12	34.12710106888394	22.345072420260596	30.544921493077453	12.982905017778009
13	19.240255144055592	36.37049043072084	29.018091982957344	15.37116244226622
14	14.09681923715673	37.89511662429872	31.98189700045994	16.026167138084602
15	14.113481818507308	23.74583091198026	45.947481196070385	16.19320607344205
16	15.485734902461932	20.76915026343403	44.048084629388384	19.69703020471565
17	15.11316784590004	21.10061169575504	35.074114060189096	28.712106398155825
18	17.6697035713007	23.47420329450906	39.72572840267813	19.13036473151211
19	25.977446301044647	22.86760270897769	30.769177804890813	20.385773185086855
20	26.961364844432083	22.037916323546018	32.38799578064882	18.612723051373077
21	18.50007849315182	28.930854418613617	32.623199821531365	19.945867266703203
22	19.854636191126694	25.343510819661297	28.49411576775942	26.307737221452594
23	16.752641914241412	30.690478092148204	29.708969426228833	22.84791056738155
24	16.09564046280664	22.98933594793563	45.02401615031025	15.891007438947478
25	15.915863603689454	25.904668001509272	39.196657017976314	18.982811376824966
26	15.764729859621196	32.003723604956356	32.291462974642585	19.940083560779865
27	14.420637915221887	35.638300802282636	30.420984937577288	19.520076344918188
28	14.230601863454966	29.060850094604906	38.10140213556456	18.60714590637557
29	14.876035903043055	23.522400843870237	38.75661339230877	22.844949860777934
30	17.453847403804577	32.68723370853978	32.998589877412975	16.860329010242666
31	24.995937635125273	29.473352263494622	30.06026070742986	15.470449393950245
32	27.258674870348592	24.093335242874335	30.12863237388078	18.51935751289629
33	26.79453247000047	28.425537540382663	26.301127271825912	18.478802717790956
34	20.251439729653057	26.504520930130077	28.72174590802806	24.522293432188803
35	20.517903323978416	26.5142981472862	32.773851590106005	20.193946938629374
36	28.042573583887147	29.177075042207285	26.447234700032222	16.33311667387335
37	18.29813076132849	36.5519197772447	29.90940237792938	15.240547083497436
38	19.255609506209222	34.05714576866829	28.41885873711404	18.26838598800845
39	20.420268859701064	35.29857693292829	27.575332769651517	16.705821437719127
40	23.34647978870194	27.25530104189331	28.670381091137436	20.72783807826731
41	17.23200096945928	26.460110331075853	30.570879316090544	25.737009383374325
42	17.626807752369253	25.81811897358499	29.357884705953634	27.197188568092123
43	18.13501648356188	25.7700591315077	29.80729242692563	26.287631958004788
44	16.556615595625317	28.831360906003763	31.832002621946685	22.78002087642424
45	15.570975711189266	39.24988088319943	25.14080569777658	20.03833770783472
46	18.699960615716808	34.78286315476371	28.12561107607226	18.391565153447225
47	18.440864361079516	30.01233857263646	28.949238341012812	22.597558725271213
48	18.551030188190772	27.382336011280984	34.31892731534142	19.74770648518683
49	21.180275359484867	28.138624414399775	33.998689026657374	16.682411199457984
50	18.68081930325623	35.79652647147118	28.881899479191052	16.64075474608154
51	17.234823968779004	32.69398136545034	28.620806468937367	21.450388196833284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1402.0
1	2643.0
2	3884.0
3	15821.0
4	27758.0
5	20450.0
6	13142.0
7	12996.0
8	12850.0
9	13060.0
10	13270.0
11	13181.0
12	13092.0
13	12870.5
14	12649.0
15	12511.5
16	12374.0
17	11866.5
18	11359.0
19	10672.0
20	9985.0
21	9796.0
22	9607.0
23	10156.5
24	10706.0
25	12010.5
26	16611.5
27	19908.0
28	24666.5
29	29425.0
30	33268.0
31	37111.0
32	42899.0
33	48687.0
34	55604.0
35	62521.0
36	66127.5
37	69734.0
38	74057.5
39	78381.0
40	92108.5
41	105836.0
42	119629.5
43	133423.0
44	130791.5
45	128160.0
46	117933.5
47	107707.0
48	93640.5
49	79574.0
50	72457.0
51	65340.0
52	60162.5
53	54985.0
54	51821.0
55	48657.0
56	47417.0
57	46177.0
58	43372.5
59	40568.0
60	37015.5
61	33463.0
62	30202.0
63	26941.0
64	23775.5
65	20610.0
66	17819.5
67	15029.0
68	13473.5
69	11918.0
70	10625.5
71	9333.0
72	7799.5
73	6266.0
74	4963.0
75	2758.0
76	1856.0
77	1377.0
78	898.0
79	646.5
80	395.0
81	323.0
82	251.0
83	163.0
84	75.0
85	59.0
86	43.0
87	29.5
88	16.0
89	11.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1452356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.895670675444403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03538432689099	19.430916015345712
2	6.793716139505118	3.5185567162215206
3	3.380150530570857	2.6259379491927475
4	2.307900620192603	2.3905853764864617
5	1.7446913189450592	2.2589975912853992
6	1.3859111574698646	2.1533459351558144
7	1.1476367119195787	2.0803175642843486
8	0.9232906195035483	1.9127383856312747
9	0.7728419114188346	1.8011933660054804
>10	5.260834876658297	26.19286458404438
>50	0.6930533997009057	12.831299085902845
>100	0.5511272019068767	21.20004761054009
>500	0.0029286952686374784	0.5028227018689919
>1k	2.6624502442158896E-4	0.07129009649424621
>5k	0.0	0.0
>10k+	2.6624502442158896E-4	1.029087021540734
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14926	1.0277094596641594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010328046291680552	0.0
2	0.0	0.0	0.0	0.03325630905921138	0.0
3	0.0	0.0	0.0	0.05556488904924137	0.0
4	0.0	0.0	0.0	0.08420800409816877	0.0
5	0.0	0.0	0.0	0.1607044002985494	0.0
6	0.0	0.0	0.0	0.2683914962998053	0.0
7	0.0	0.0	0.0	0.354389695088532	0.0
8	0.0	0.0	0.0	0.5894560286871814	0.0
9	0.0	0.0	0.0	0.706094098141227	0.0
10	0.0	0.0	0.0	0.8655591328847748	0.0
11	0.0	0.0	0.0	0.9713183269115837	0.0
12	0.0	0.0	0.0	1.0781103255675606	0.0
13	0.0	0.0	0.0	1.1221078027701197	0.0
14	0.0	0.0	0.0	1.1471705284379312	0.0
15	0.0	0.0	0.0	1.1718201322540756	0.0
16	0.0	0.0	0.0	1.2167127068019137	0.0
17	0.0	0.0	0.0	1.2693857428894844	0.0
18	0.0	0.0	0.0	1.3461575536576431	0.0
19	0.0	0.0	0.0	1.3818237401849134	0.0
20	0.0	0.0	0.0	1.42471955911636	0.0
21	0.0	0.0	0.0	1.4832451547692163	0.0
22	0.0	0.0	0.0	1.5465905053581903	0.0
23	0.0	0.0	0.0	1.6142736353896703	0.0
24	0.0	0.0	0.0	1.6701827926486343	0.0
25	0.0	0.0	0.0	1.7177606592323094	0.0
26	0.0	0.0	0.0	1.767059866864598	0.0
27	0.0	0.0	0.0	1.8226247559138393	0.0
28	0.0	0.0	0.0	1.8840422045283662	0.0
29	0.0	0.0	0.0	1.9464236041301168	0.0
30	0.0	0.0	0.0	2.0479827259983088	0.0
31	0.0	0.0	0.0	2.1260627559634138	0.0
32	0.0	0.0	0.0	2.202077176670183	0.0
33	0.0	0.0	0.0	2.2814654258322338	0.0
34	0.0	0.0	0.0	2.373385037828191	0.0
35	0.0	0.0	0.0	2.4815541093230586	0.0
36	0.0	0.0	0.0	2.5781557689712438	0.0
37	0.0	0.0	0.0	2.672003282941648	0.0
38	0.0	0.0	0.0	2.7697754545028905	0.0
39	0.0	0.0	0.0	2.8716788445808055	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.033902E-4	45.0	1
TATGCGA	20	7.033902E-4	45.0	12
ATCGTTA	20	7.033902E-4	45.0	35
CGTTTTT	10820	0.0	43.565155	1
CGGTCTA	225	0.0	43.0	31
ACGTAGG	265	0.0	42.45283	2
TGCGTAG	75	0.0	42.000004	1
ACGGTCT	235	0.0	41.17021	30
GACGTAG	110	0.0	40.909092	1
TACGTTG	55	6.184564E-11	40.909092	1
TCACGAC	255	0.0	40.588234	25
TATACGG	240	0.0	40.312504	2
CATACGA	1035	0.0	40.217392	18
CGTTCGA	185	0.0	40.135136	14
CGTAAGG	275	0.0	40.090908	2
ACGGGAC	320	0.0	40.078125	5
AATACGG	90	0.0	40.0	2
AAGGGAC	1185	0.0	39.87342	5
ACATACG	1050	0.0	39.85714	17
TCGTAAG	130	0.0	39.80769	1
>>END_MODULE