FastQCFastQC Report
Thu 26 May 2016
SRR1545144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545144_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2748202
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64910.23619078946889638No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC44450.16174211357098206No Hit
GTTATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA43080.15675703605484603No Hit
GGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATG38600.14045546870281006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG302.1666092E-645.00000437
CTGTCCG302.1666092E-645.0000041
AGATCCG650.045.0000041
GTCGAAC600.045.0000049
GGTCGAA600.045.0000048
CGACGAA1300.045.00000420
CGTGCGG650.045.0000042
TATATCG302.1666092E-645.00000412
TCGTGCG600.045.0000041
TACGACG600.045.0000041
CGTAAGC302.1666092E-645.00000441
CGTAACG302.1666092E-645.0000042
ACGTATG1300.045.0000041
CACGTTA302.1666092E-645.00000435
TGTACCG302.1666092E-645.0000041
TATCGTG600.045.0000041
ATCGACG650.045.0000041
ATTTACG207.035002E-445.01
TATCACG1050.045.01
CTAGACG1700.045.01
Produced by FastQC (version 0.11.5)