FastQCFastQC Report
Thu 26 May 2016
SRR1545143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545143_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2429936
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132800.5465164514620962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG207.034841E-445.01
CTATGCG351.2126839E-745.01
CGTTCGA207.034841E-445.014
CGGGCTA351.2126839E-745.06
TCGCTAC207.034841E-445.029
TCGTAGC253.892094E-545.011
TAGGCGT207.034841E-445.02
CACGTCA406.8212103E-945.035
ATCGCTA207.034841E-445.028
TCGAAGT207.034841E-445.021
TACCGGC253.892094E-545.03
ACCGGTA302.1665055E-644.99999637
GACCGTA302.1665055E-644.9999969
CCGTACA302.1665055E-644.99999632
CGTACAC302.1665055E-644.99999633
AGTACGG2000.043.8752
CGTTTTT121300.042.9039571
TGGGCGA7250.042.2068946
TACGGGA7950.041.886794
CGTCGGG700.041.7857133
Produced by FastQC (version 0.11.5)