FastQCFastQC Report
Thu 26 May 2016
SRR1545142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545142_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1088851
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43590.4003302563895335No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22240.20425200509527935No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12530.11507543272679183No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12020.11039159627901339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGC650.045.00000440
ACCGACG453.8562575E-1045.00000445
ATATGCG750.045.0000041
ACGTGCC650.045.00000441
CTCGAGG650.045.0000042
CGGTCTA453.8562575E-1045.00000431
AATCGCG650.045.0000041
ACGGTCT453.8562575E-1045.00000430
GTGCACG406.8139343E-945.01
CTCACGT502.1827873E-1145.045
TCGCAAG351.2118471E-745.01
ACTGCGT253.89067E-545.043
GTCGATG253.89067E-545.01
GTCGATA207.033124E-445.07
CGTATCT253.89067E-545.036
GATCGCA253.89067E-545.09
GCGAATT207.033124E-445.026
ACGCATG253.89067E-545.01
GCGAACG207.033124E-445.01
TAGGTCG207.033124E-445.04