FastQCFastQC Report
Thu 26 May 2016
SRR1545141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545141_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2437119
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430051.764583510284069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT294450.044.0295451
ACGGGTA1900.043.815795
CGGTCTA3000.040.50000431
TAATGCG1000.040.51
ATGGGAC23850.040.05
TTGGGAC17600.039.8863645
TGGGACA24350.039.5482566
GTTTTTT340700.039.379952
CCCTAGC51500.039.3203921
ATACTAT50950.038.94994745
GATGCCA46100.038.801529
GGGCGAT35600.038.7429777
GCCCTAG52750.038.7298620
ACTAATC53000.038.6745310
TACTCGA356.2511717E-638.57142644
ATCCTAG53450.038.51730714
GGACTAA56150.038.508468
GACTAAT52800.038.3948869
TACACAA52700.038.33965730
GGATGCC47400.038.306968
Produced by FastQC (version 0.11.5)