##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545141_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2437119
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.737805991418558	31.0	30.0	31.0	28.0	33.0
2	31.048130189785564	31.0	31.0	33.0	28.0	34.0
3	30.956460886809385	31.0	31.0	34.0	28.0	34.0
4	34.9945907442353	35.0	35.0	37.0	32.0	37.0
5	34.97715047972627	35.0	35.0	37.0	32.0	37.0
6	35.07709184492017	37.0	35.0	37.0	32.0	37.0
7	35.399766691737256	35.0	35.0	37.0	35.0	37.0
8	35.43170727403955	36.0	35.0	37.0	35.0	37.0
9	37.24805395222802	39.0	37.0	39.0	35.0	39.0
10	36.706929370293366	39.0	37.0	39.0	32.0	39.0
11	36.43118042245783	38.0	35.0	39.0	32.0	39.0
12	36.14787378047605	38.0	35.0	39.0	32.0	39.0
13	35.9846322645714	38.0	35.0	39.0	31.0	39.0
14	36.92459416220546	39.0	35.0	40.0	31.0	41.0
15	37.036175090342326	39.0	35.0	40.0	31.0	41.0
16	36.96302314330978	39.0	35.0	40.0	31.0	41.0
17	36.71007940112896	38.0	35.0	40.0	31.0	41.0
18	36.591683048714486	38.0	35.0	40.0	31.0	41.0
19	36.39126443969293	37.0	35.0	40.0	30.0	41.0
20	36.12398738018127	37.0	34.0	40.0	30.0	41.0
21	36.0403923649194	37.0	34.0	40.0	30.0	41.0
22	35.89135491537344	37.0	34.0	40.0	30.0	41.0
23	35.66160577304596	37.0	34.0	40.0	29.0	41.0
24	35.5829703022298	37.0	34.0	40.0	29.0	41.0
25	35.34324667773711	36.0	34.0	40.0	29.0	41.0
26	35.02857020933323	36.0	34.0	40.0	27.0	41.0
27	35.04791600246028	36.0	34.0	40.0	27.0	41.0
28	34.943181682962546	36.0	33.0	40.0	26.0	41.0
29	34.82214820039563	36.0	33.0	40.0	26.0	41.0
30	34.74796593847079	36.0	33.0	40.0	25.0	41.0
31	34.59024651648114	36.0	33.0	40.0	25.0	41.0
32	34.35453869917718	36.0	33.0	40.0	23.0	41.0
33	33.90298750286711	36.0	33.0	40.0	21.0	41.0
34	33.362439421300316	36.0	32.0	40.0	18.0	41.0
35	32.93635928323565	36.0	31.0	40.0	16.0	41.0
36	32.74510108041503	36.0	31.0	40.0	15.0	41.0
37	32.71324912735078	36.0	31.0	40.0	15.0	41.0
38	32.566322366696085	35.0	31.0	40.0	15.0	41.0
39	32.453495295059454	35.0	31.0	40.0	15.0	41.0
40	32.27132856458794	35.0	30.0	40.0	13.0	41.0
41	32.107016932697995	35.0	30.0	40.0	12.0	41.0
42	32.03797147369497	35.0	30.0	40.0	11.0	41.0
43	31.901114389572278	35.0	30.0	40.0	10.0	41.0
44	31.771335745197504	35.0	30.0	39.0	10.0	41.0
45	31.789329942444336	35.0	30.0	39.0	10.0	41.0
46	31.758235441108948	35.0	30.0	39.0	10.0	41.0
47	31.63175823585143	35.0	29.0	39.0	10.0	41.0
48	31.619653369408717	35.0	29.0	39.0	10.0	41.0
49	31.554234733716328	35.0	29.0	39.0	10.0	41.0
50	31.422365506157064	35.0	29.0	39.0	10.0	41.0
51	31.13147490951406	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	1.0
13	12.0
14	34.0
15	109.0
16	350.0
17	1082.0
18	2538.0
19	5027.0
20	8711.0
21	13621.0
22	20213.0
23	28842.0
24	41450.0
25	58917.0
26	75872.0
27	83467.0
28	80373.0
29	78693.0
30	85514.0
31	96207.0
32	113908.0
33	139154.0
34	192662.0
35	238755.0
36	203979.0
37	243398.0
38	311094.0
39	313088.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.63887360444853	20.63662053432762	24.540943630573643	23.183562230650207
2	39.493803954587364	22.695239748243726	26.321652738335715	11.489303558833196
3	26.271470535497038	23.84458863108449	37.52319849789854	12.36074233551993
4	23.31568544662776	25.60289423700689	37.19416245164886	13.887257864716496
5	20.497809093441887	28.795434281214828	37.095275199938946	13.61148142540434
6	18.62568056791646	38.25660544273792	33.75620968857081	9.36150430077481
7	79.68974842836973	3.325812157715729	15.412296239945608	1.5721431739689362
8	80.34195293705395	2.750296559174993	15.358421152188301	1.54932935158275
9	75.92809378614668	4.616106148284102	16.875335180596434	2.5804648849727894
10	36.88449353519463	26.949853495048863	23.676275142904387	12.489377826852115
11	26.49127925226466	21.689995441338727	33.074051779991045	18.744673526405563
12	25.788564284304545	21.687041133403824	36.62488372541513	15.8995108568765
13	18.67984288005633	26.953177091475634	35.561948349670246	18.805031678797793
14	15.855647590454138	28.83059875205109	36.022451099023066	19.291302558471703
15	16.13388595304538	21.798976578492884	42.777722384503996	19.28941508395774
16	16.91431563251528	20.941406636278327	40.11724499296095	22.027032738245445
17	17.23502217167073	20.953141803908633	37.61232012060142	24.199515903819222
18	18.66892835351905	21.909106613177283	38.62244724200993	20.799517791293738
19	22.56221382706384	22.620602440832805	32.05202536273362	22.765158369369733
20	24.082820740390602	22.26021790482943	34.23587440744584	19.421086947334125
21	19.754103102885004	24.201690602715747	33.73068775057763	22.31351854382162
22	20.767389692501677	23.62207179870987	31.61675732699142	23.99378118179703
23	18.82813272556654	26.480446789836687	32.6083379596975	22.08308252489928
24	19.532940328313884	21.73447418858086	41.099757541589064	17.63282794151619
25	18.625762632025765	25.10238523436894	35.37808371277726	20.893768420828035
26	18.549771266811348	25.27820758855025	34.777046176243346	21.394974968395058
27	16.76582062673181	26.04062419602818	33.75854851568594	23.435006661554073
28	16.928430659315364	23.00458040826074	37.0781648331493	22.988824099274595
29	16.875253116487134	20.34332340767931	39.25233031296379	23.529093162869767
30	19.962751100787447	25.380090180249717	35.972515088512296	18.684643630450545
31	22.733932975780007	26.868527962729765	32.12547274055965	18.272066320930573
32	23.7737262727015	22.51141614340539	30.94342131016171	22.771436273731403
33	23.094850928493848	27.58293706626554	29.221059784113944	20.101152221126668
34	21.115628740328233	24.689643796630364	30.959464843530416	23.235262619510987
35	23.142899464490654	25.995488935911627	31.35095167695956	19.510659922638165
36	24.489653562259374	30.039854434682915	27.551014127746736	17.91947787531097
37	19.92697114913141	31.330066361141988	31.083340616523035	17.659621873203566
38	21.64961989956174	28.77463102950656	30.887822876109045	18.68792619482266
39	22.40034237146401	31.054905402649602	27.8633501277533	18.68140209813308
40	23.18590105776534	26.203644549158252	29.30501136793074	21.305443025145674
41	19.14867513650339	25.71798915030411	31.401051815688934	23.73228389750357
42	19.079495092361103	26.20249565162801	29.708110272826232	25.00989898318465
43	19.186875979383856	25.636458457711747	29.91257300115423	25.26409256175016
44	19.015936439706064	26.667758119320396	30.00678259863388	24.309522842339664
45	18.11844230831568	31.508843023258198	26.805461694730543	23.56725297369558
46	19.933659374039596	29.181135594938123	30.302993001162438	20.582212029859846
47	19.124876544805566	27.543997646401348	32.021292353799716	21.309833454993377
48	20.07657402039047	24.958608914870386	32.62934637167902	22.335470693060124
49	22.153493530681104	25.92889391121238	32.219559241875345	19.698053316231174
50	19.76645375133508	30.01679441996882	31.36227652404335	18.85447530465275
51	18.436112475426928	27.79626271839824	30.429823082089957	23.337801724084873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2152.0
1	3691.5
2	5231.0
3	39356.5
4	73482.0
5	52712.5
6	31943.0
7	31054.5
8	30166.0
9	30707.0
10	31248.0
11	31086.5
12	30925.0
13	30691.5
14	30458.0
15	30088.0
16	29718.0
17	28117.5
18	26517.0
19	23381.5
20	20246.0
21	18404.5
22	16563.0
23	16732.5
24	16902.0
25	18793.0
26	22962.0
27	25240.0
28	28384.5
29	31529.0
30	37703.5
31	43878.0
32	53102.5
33	62327.0
34	74710.5
35	87094.0
36	89570.0
37	92046.0
38	97007.0
39	101968.0
40	113394.0
41	124820.0
42	132629.5
43	140439.0
44	141472.0
45	142505.0
46	137311.5
47	132118.0
48	129109.5
49	126101.0
50	121963.5
51	117826.0
52	120033.0
53	122240.0
54	126421.5
55	130603.0
56	128898.5
57	127194.0
58	116767.0
59	106340.0
60	96508.5
61	86677.0
62	80861.5
63	75046.0
64	67334.5
65	59623.0
66	51647.0
67	43671.0
68	38229.0
69	32787.0
70	28021.5
71	23256.0
72	19739.0
73	16222.0
74	13063.0
75	7242.0
76	4580.0
77	3579.0
78	2578.0
79	1921.0
80	1264.0
81	876.0
82	488.0
83	375.0
84	262.0
85	191.0
86	120.0
87	87.5
88	55.0
89	53.5
90	52.0
91	37.5
92	23.0
93	15.0
94	7.0
95	3.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2437119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.53474258983567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89122990074422	19.606847650626165
2	6.730539952784424	3.571862902754788
3	3.196605544367496	2.5446331584309907
4	2.1987696087758377	2.3337514233288212
5	1.6525972791895458	2.1925621703978693
6	1.3102945903048062	2.0860997802355334
7	1.1415906396462088	2.1204269636184594
8	0.9318820513272312	1.9781800284844886
9	0.8098261788916683	1.933967627946057
>10	7.2076249323167	38.13565719592133
>50	0.7448603734759696	13.693331907495429
>100	0.17964573768230319	6.831742037803945
>500	0.002188446445249646	0.3919715759002967
>1k	0.002188446445249646	0.7951838181570992
>5k	0.0	0.0
>10k+	1.5631760323211755E-4	1.7837817588986518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43005	1.764583510284069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012350648450075684	0.0
2	0.0	0.0	0.0	0.04312468943863636	0.0
3	0.0	0.0	0.0	0.07398079453649986	0.0
4	0.0	0.0	0.0	0.10249807251923275	0.0
5	0.0	0.0	0.0	0.18210025854297635	0.0
6	0.0	0.0	0.0	0.35570688177311	0.0
7	0.0	0.0	0.0	0.4932463289646505	0.0
8	0.0	0.0	0.0	0.734104489768452	0.0
9	0.0	0.0	0.0	0.9005305034345882	0.0
10	0.0	0.0	0.0	1.067613029975147	0.0
11	0.0	0.0	0.0	1.1736808912490526	0.0
12	0.0	0.0	0.0	1.2574273147925892	0.0
13	0.0	0.0	0.0	1.3016188376521622	0.0
14	0.0	0.0	0.0	1.3401889690244917	0.0
15	0.0	0.0	0.0	1.3675163174223335	0.0
16	0.0	0.0	0.0	1.404814455100469	0.0
17	0.0	0.0	0.0	1.4465440546809574	0.0
18	0.0	0.0	0.0	1.5126466947243857	0.0
19	0.0	0.0	0.0	1.5400971392861817	0.0
20	0.0	0.0	0.0	1.5702556994549712	0.0
21	0.0	0.0	0.0	1.6057073946737932	0.0
22	0.0	0.0	0.0	1.648093507128704	0.0
23	0.0	0.0	0.0	1.6993425433883205	0.0
24	0.0	0.0	0.0	1.7458318613083728	0.0
25	0.0	0.0	0.0	1.7884231340365406	0.0
26	0.0	0.0	0.0	1.8285935155402753	0.0
27	0.0	0.0	0.0	1.876806179755687	0.0
28	0.0	0.0	0.0	1.9216542154896827	0.0
29	0.0	0.0	0.0	1.973190476131859	0.0
30	0.0	0.0	0.0	2.0437656101322914	0.0
31	0.0	0.0	0.0	2.0976817299442496	0.0
32	0.0	0.0	0.0	2.1534442922155215	0.0
33	0.0	0.0	0.0	2.223937362106651	0.0
34	0.0	0.0	0.0	2.296523066785003	0.0
35	0.0	0.0	0.0	2.379202656907603	0.0
36	0.0	0.0	0.0	2.449777790908035	0.0
37	0.0	0.0	0.0	2.517111392591006	0.0
38	0.0	0.0	0.0	2.591174251236809	0.0
39	0.0	0.0	0.0	2.681485803524571	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	29445	0.0	44.029545	1
ACGGGTA	190	0.0	43.81579	5
CGGTCTA	300	0.0	40.500004	31
TAATGCG	100	0.0	40.5	1
ATGGGAC	2385	0.0	40.0	5
TTGGGAC	1760	0.0	39.886364	5
TGGGACA	2435	0.0	39.548256	6
GTTTTTT	34070	0.0	39.37995	2
CCCTAGC	5150	0.0	39.32039	21
ATACTAT	5095	0.0	38.949947	45
GATGCCA	4610	0.0	38.80152	9
GGGCGAT	3560	0.0	38.742977	7
GCCCTAG	5275	0.0	38.72986	20
ACTAATC	5300	0.0	38.67453	10
TACTCGA	35	6.2511717E-6	38.571426	44
ATCCTAG	5345	0.0	38.517307	14
GGACTAA	5615	0.0	38.50846	8
GACTAAT	5280	0.0	38.394886	9
TACACAA	5270	0.0	38.339657	30
GGATGCC	4740	0.0	38.30696	8
>>END_MODULE