FastQCFastQC Report
Thu 26 May 2016
SRR1545140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545140_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2381836
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159800.6709110115054101No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG60150.2525362787362354No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG37970.15941483796533432No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31350.13162115275778852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAA207.0348114E-445.00000428
CACGATA207.0348114E-445.00000422
CGAATCG207.0348114E-445.00000412
ACGAACT207.0348114E-445.00000442
TGTAACG351.2126657E-745.01
ACGTTAG600.044.9999961
CGCTAAT302.1664873E-644.99999611
GACGACT253.8920713E-544.9999964
TCGAATA253.8920713E-544.99999623
CGGTCTA2000.042.74999631
ACGGGAC2850.042.631585
CGTTTTT124800.042.169471
TCACGAC2100.041.78571725
CTCACGA2100.041.78571724
TAGTTCG700.041.7857131
AATGCGG4100.041.7073172
ATATCGA650.041.53846414
GCTACGA1900.041.4473710
ACGGGAT7550.041.423845
TTGTGCG1250.041.3999981
Produced by FastQC (version 0.11.5)