FastQCFastQC Report
Thu 26 May 2016
SRR1545139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545139_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1313772
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.47793681095349877No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21770.16570607380884964No Hit
TTTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA18850.14347999500674394No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18440.14035920996946197No Hit
TTGATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA14720.11204379450924513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCG302.1657397E-645.00000443
GTCGACG302.1657397E-645.0000041
GCGAATT551.8189894E-1245.00000414
TCTTCGA302.1657397E-645.00000419
GACCGTA302.1657397E-645.00000429
ACGGGCC453.8562575E-1045.0000045
ACGGACC551.8189894E-1245.00000439
GCGATAT900.045.0000049
TCGTAAG650.045.0000041
GGACCGT302.1657397E-645.0000048
GTGCGTA302.1657397E-645.00000416
CGTCAAT302.1657397E-645.00000443
GCCGTGC302.1657397E-645.00000432
CGGACCA551.8189894E-1245.00000440
CTAGACG406.8157533E-945.01
CTATGCG351.2121018E-745.01
TCGCATC207.033656E-445.021
TCGCAAA207.033656E-445.027
GCGCACA207.033656E-445.09
CATTACG207.033656E-445.038