##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545139_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1313772
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.967047554674632	31.0	31.0	33.0	30.0	33.0
2	31.30090076512515	31.0	31.0	33.0	30.0	34.0
3	31.43032200412248	31.0	31.0	34.0	30.0	34.0
4	35.306138355818206	37.0	35.0	37.0	33.0	37.0
5	35.20769205006653	37.0	35.0	37.0	32.0	37.0
6	35.270594897744814	37.0	35.0	37.0	32.0	37.0
7	35.59699171545748	36.0	35.0	37.0	35.0	37.0
8	35.64714196983952	37.0	35.0	37.0	35.0	37.0
9	37.48985364279342	39.0	37.0	39.0	35.0	39.0
10	37.046524815569214	39.0	37.0	39.0	33.0	39.0
11	36.64107927402928	39.0	35.0	39.0	32.0	39.0
12	35.37868519042878	35.0	35.0	39.0	31.0	39.0
13	34.973800629028474	35.0	34.0	39.0	30.0	39.0
14	35.66881011317032	36.0	34.0	40.0	30.0	41.0
15	36.08575460582202	36.0	35.0	40.0	31.0	41.0
16	36.240094171591416	36.0	35.0	40.0	32.0	41.0
17	36.084514664645006	36.0	35.0	40.0	31.0	41.0
18	36.182246234506444	36.0	35.0	40.0	31.0	41.0
19	36.05669781362367	36.0	35.0	40.0	31.0	41.0
20	35.77534610267231	36.0	34.0	40.0	31.0	41.0
21	35.65545239204367	35.0	34.0	40.0	31.0	41.0
22	35.48596027316764	35.0	34.0	40.0	30.0	41.0
23	35.41677399122526	35.0	34.0	40.0	30.0	41.0
24	35.496940869496385	35.0	34.0	40.0	31.0	41.0
25	35.4074641566421	35.0	34.0	40.0	30.0	41.0
26	35.16318508843239	35.0	34.0	39.0	30.0	41.0
27	35.16140624096114	35.0	34.0	40.0	30.0	41.0
28	35.237175856998014	36.0	34.0	40.0	30.0	41.0
29	35.230434200150405	36.0	34.0	40.0	30.0	41.0
30	35.195702907353784	36.0	34.0	40.0	30.0	41.0
31	34.96723480177687	35.0	34.0	40.0	29.0	41.0
32	34.80646109066109	35.0	34.0	40.0	29.0	41.0
33	34.53954643575902	35.0	33.0	40.0	27.0	41.0
34	34.311520568256896	35.0	34.0	40.0	26.0	41.0
35	34.09315543336287	35.0	33.0	40.0	24.0	41.0
36	33.84027061012109	35.0	33.0	40.0	23.0	41.0
37	33.87287520208986	35.0	33.0	40.0	23.0	41.0
38	33.9238794859382	35.0	33.0	40.0	24.0	41.0
39	33.900457613649856	35.0	33.0	40.0	23.0	41.0
40	33.57347774195218	35.0	33.0	39.0	23.0	41.0
41	33.663878511644334	35.0	33.0	39.0	23.0	41.0
42	33.66136209327037	35.0	33.0	39.0	23.0	41.0
43	33.63096412467308	35.0	33.0	39.0	23.0	41.0
44	33.60339617528764	35.0	33.0	39.0	23.0	41.0
45	33.656747137250605	35.0	33.0	39.0	23.0	41.0
46	33.6607600101083	35.0	33.0	39.0	23.0	41.0
47	33.61637026820483	35.0	33.0	39.0	23.0	41.0
48	33.6546014072457	35.0	33.0	39.0	23.0	41.0
49	33.68652551584293	35.0	33.0	39.0	24.0	41.0
50	33.4943734529279	35.0	33.0	39.0	24.0	41.0
51	33.17943828913997	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	3.0
14	9.0
15	26.0
16	92.0
17	238.0
18	658.0
19	1354.0
20	2561.0
21	3951.0
22	6191.0
23	9175.0
24	13312.0
25	18822.0
26	24623.0
27	28404.0
28	30421.0
29	34663.0
30	41143.0
31	50820.0
32	64879.0
33	86784.0
34	160063.0
35	239328.0
36	77208.0
37	93667.0
38	134870.0
39	190428.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.48961311399543	24.20229689778744	31.50287873390512	13.805211254312011
2	31.37066401171588	26.350310403936145	30.127678166378946	12.151347417969022
3	27.934984152501347	25.59957131069927	34.16445167045728	12.300992866342105
4	25.30812043490042	29.051083445224897	32.380732729880066	13.260063389994611
5	22.7908647771455	32.664571934856276	32.23466476679363	12.309898521204593
6	20.14162274732602	40.92932411407763	29.696553130984675	9.232500007611671
7	85.6421814439644	4.91782440179879	8.118303632593783	1.3216905216430248
8	87.67350803640205	3.456992537517926	7.664648051564503	1.2048513745155172
9	83.23110859418529	5.25555423619928	9.4382434699476	2.0750936996678266
10	57.0563233194192	21.270890230572732	14.557929381962776	7.114857068045293
11	54.30074624820745	15.803959895628768	21.371364285431564	8.523929570732212
12	50.01339654064785	20.45050434930871	21.890251885410862	7.645847224632584
13	18.66069607207339	50.61144551718259	20.184476454057474	10.543381956686549
14	10.314499015049796	49.276358454891714	27.406962547534885	13.002179982523604
15	9.035662200138228	23.10142094670917	56.73823159574112	11.124685257411484
16	9.865029852972967	16.80801539384307	54.74800802574572	18.578946727438247
17	10.474191868908761	18.71382553441541	31.13021133042872	39.68177126624711
18	16.08391714848543	23.31797298161325	43.924744932910734	16.67336493699059
19	31.87623118775556	19.838526015168537	29.032663201834108	19.25257959524179
20	31.63250548801466	20.11087159720256	31.11475963865876	17.141863276124013
21	16.152422185889183	34.89235575122624	31.6617343039736	17.293487758910985
22	19.312331211199506	24.357042165611688	22.576139543238856	33.754487079949946
23	13.533931306193159	33.990677225576434	27.080041285702546	25.395350182527864
24	11.088986521253307	20.70785493982213	55.02857421226819	13.174584326656374
25	10.47799770432008	25.56539490870562	47.549498695359624	16.40710869161468
26	10.012239566682805	41.14435381481718	30.10141790204084	18.741988716459172
27	9.718581306345394	49.40073315613363	27.350255599906227	13.530429937614747
28	9.303745246511571	33.39384611637331	43.41430628754457	13.88810234957055
29	9.462905283412953	25.235505095252446	42.05082769308525	23.250761928249347
30	13.827970150071703	42.58722213595662	30.15903825016822	13.42576946380346
31	31.194986649129376	30.653264036682167	26.643968664273558	11.507780649914903
32	34.948682115313765	22.577052943737574	29.28856757489123	13.185697366057427
33	34.93833024299498	27.231894118614186	21.486833331811	16.342942306579832
34	17.93591277634171	26.461060214405546	26.18095072813243	29.42207628112032
35	18.983963731910862	24.8301075072387	36.334995722241	19.85093303860944
36	36.53213799654735	27.997780436788116	23.35587910230999	12.114202464354545
37	15.95847681332834	44.85451052389608	27.965963652749487	11.221049010026093
38	15.627673599376452	42.376226620753066	25.48844091668874	16.50765886318174
39	19.139698516942058	39.67035376001316	27.85277810761685	13.337169615427943
40	27.69826118991728	24.704819405498064	26.600886607417422	20.996032797167242
41	13.783289642342812	24.22185889180162	29.352962310050756	32.64188915580481
42	15.226843013856287	20.99329259567109	27.801779913105168	35.97808447736745
43	16.608970201831063	22.38683728987983	29.474520693088298	31.52967181520081
44	11.978562490295118	31.460101143881893	35.31084541305493	21.25049095276806
45	11.359657535706349	51.22974153810554	22.35730400708799	15.053296919100118
46	17.413371574367545	43.12940144865319	24.061557104276847	15.395669872702417
47	16.954007240221287	29.92574053945433	26.521877464278425	26.59837475604595
48	17.15952235243254	26.992202604409286	40.53717083329528	15.311104209862899
49	20.828576039069183	28.474803847242903	38.137211022917214	12.559409090770696
50	18.096214563866486	43.42290747557415	25.890946069789887	12.58993189076948
51	15.808374664705902	37.87331439549633	26.34505835106853	19.973252588729245
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1272.0
1	2459.5
2	3647.0
3	8771.0
4	13895.0
5	10707.0
6	7519.0
7	7665.0
8	7811.0
9	8004.0
10	8197.0
11	8062.5
12	7928.0
13	7691.0
14	7454.0
15	7144.0
16	6834.0
17	6199.5
18	5565.0
19	5211.0
20	4857.0
21	5258.5
22	5660.0
23	6609.0
24	7558.0
25	8841.0
26	13191.5
27	16259.0
28	22849.0
29	29439.0
30	31851.5
31	34264.0
32	42767.0
33	51270.0
34	55011.0
35	58752.0
36	61088.0
37	63424.0
38	74116.0
39	84808.0
40	111858.0
41	138908.0
42	165087.0
43	191266.0
44	178762.5
45	166259.0
46	144530.0
47	122801.0
48	104208.0
49	85615.0
50	72277.5
51	58940.0
52	45954.0
53	32968.0
54	25712.0
55	18456.0
56	16104.0
57	13752.0
58	13196.5
59	12641.0
60	11455.0
61	10269.0
62	9099.5
63	7930.0
64	6889.0
65	5848.0
66	4923.5
67	3999.0
68	3300.0
69	2601.0
70	2236.0
71	1871.0
72	1594.0
73	1317.0
74	1127.0
75	683.0
76	429.0
77	316.0
78	203.0
79	156.5
80	110.0
81	87.5
82	65.0
83	48.0
84	31.0
85	20.5
86	10.0
87	6.5
88	3.0
89	2.5
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1313772.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.125493038921277
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2186526110464	14.358771364542445
2	7.2212382984476475	2.6177700902180914
3	2.8676682354711183	1.5593370191990226
4	1.7410335388067342	1.2622836515267974
5	1.1891853898380107	1.0777285752747876
6	0.8576556482123745	0.9327258888878948
7	0.7142352204144948	0.9062105861042733
8	0.5623995004033762	0.815501458372336
9	0.456404043375439	0.7445293480023341
>10	3.2302339114968284	11.649363358537952
>50	0.6100727619893612	8.026411845288436
>100	1.2937749951112876	50.81434256215238
>500	0.03407992670049245	3.9126555701371566
>1k	0.0029451788506598417	0.8435247975146588
>5k	4.2073983580854883E-4	0.47884388424143776
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6279	0.47793681095349877	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2177	0.16570607380884964	No Hit
TTTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1885	0.14347999500674394	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1844	0.14035920996946197	No Hit
TTGATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1472	0.11204379450924513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011417506233958404	0.0
2	0.0	0.0	0.0	0.05526073017235868	0.0
3	0.0	0.0	0.0	0.0879909147097061	0.0
4	0.0	0.0	0.0	0.13183413864810636	0.0
5	0.0	0.0	0.0	0.23938704737199454	0.0
6	0.0	0.0	0.0	0.3511263750483341	0.0
7	0.0	0.0	0.0	0.42503569873615815	0.0
8	0.0	0.0	0.0	0.6084769655617566	0.0
9	0.0	0.0	0.0	0.6947933126904821	0.0
10	0.0	0.0	0.0	0.8415463261509607	0.0
11	0.0	0.0	0.0	0.9636375261460893	0.0
12	0.0	0.0	0.0	1.0791826892337484	0.0
13	0.0	0.0	0.0	1.1183066772621124	0.0
14	0.0	0.0	0.0	1.1355090533212764	0.0
15	0.0	0.0	0.0	1.1591813495796834	0.0
16	0.0	0.0	0.0	1.2215970503253228	0.0
17	0.0	0.0	0.0	1.293984039848619	0.0
18	0.0	0.0	0.0	1.4090725026869197	0.0
19	0.0	0.0	0.0	1.4583200129093936	0.0
20	0.0	0.0	0.0	1.5139613266228844	0.0
21	0.0	0.0	0.0	1.5880990004353877	0.0
22	0.0	0.0	0.0	1.6629978413301547	0.0
23	0.0	0.0	0.0	1.7462695201298246	0.0
24	0.0	0.0	0.0	1.811425422371614	0.0
25	0.0	0.0	0.0	1.865011584962992	0.0
26	0.0	0.0	0.0	1.9222513495492368	0.0
27	0.0	0.0	0.0	1.977816546554501	0.0
28	0.0	0.0	0.0	2.041526231339989	0.0
29	0.0	0.0	0.0	2.115816138568945	0.0
30	0.0	0.0	0.0	2.21689912709359	0.0
31	0.0	0.0	0.0	2.305270625344428	0.0
32	0.0	0.0	0.0	2.3857259859397217	0.0
33	0.0	0.0	0.0	2.465952996410336	0.0
34	0.0	0.0	0.0	2.5570646961573242	0.0
35	0.0	0.0	0.0	2.691182336052222	0.0
36	0.0	0.0	0.0	2.79051464028766	0.0
37	7.611670822638936E-5	0.0	0.0	2.894642297141361	0.0
38	7.611670822638936E-5	0.0	0.0	2.9964103360400434	0.0
39	7.611670822638936E-5	0.0	0.0	3.101908093641819	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	30	2.1657397E-6	45.000004	43
GTCGACG	30	2.1657397E-6	45.000004	1
GCGAATT	55	1.8189894E-12	45.000004	14
TCTTCGA	30	2.1657397E-6	45.000004	19
GACCGTA	30	2.1657397E-6	45.000004	29
ACGGGCC	45	3.8562575E-10	45.000004	5
ACGGACC	55	1.8189894E-12	45.000004	39
GCGATAT	90	0.0	45.000004	9
TCGTAAG	65	0.0	45.000004	1
GGACCGT	30	2.1657397E-6	45.000004	8
GTGCGTA	30	2.1657397E-6	45.000004	16
CGTCAAT	30	2.1657397E-6	45.000004	43
GCCGTGC	30	2.1657397E-6	45.000004	32
CGGACCA	55	1.8189894E-12	45.000004	40
CTAGACG	40	6.8157533E-9	45.0	1
CTATGCG	35	1.2121018E-7	45.0	1
TCGCATC	20	7.033656E-4	45.0	21
TCGCAAA	20	7.033656E-4	45.0	27
GCGCACA	20	7.033656E-4	45.0	9
CATTACG	20	7.033656E-4	45.0	38
>>END_MODULE