FastQCFastQC Report
Thu 26 May 2016
SRR1545138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545138_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2209950
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147480.6673454150546393No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG32550.14728840019004955No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT29250.1323559356546528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG800.045.0000041
TGTATCG650.045.0000041
TACGGAC207.034701E-445.00000441
CGTTAGT207.034701E-445.00000425
CCCGTAG207.034701E-445.00000424
CGTAGAT207.034701E-445.00000441
CACTACG406.8193913E-945.0000041
TATTACG650.045.0000041
ACGTCCA207.034701E-445.00000414
ACCGTAA207.034701E-445.00000424
CCACGTA207.034701E-445.00000410
TCGAACA207.034701E-445.00000444
ATTCGAT351.2126111E-745.014
GCCGATA453.8562575E-1045.09
GTGTCGA253.891979E-545.08
GACGGTT502.1827873E-1145.028
TCGATTG600.044.9999961
TCCGTAA302.1664146E-644.99999612
TACGAAG1150.043.043481
TCGCGCA1050.042.85714334