##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545138_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2209950
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.852972239190933	31.0	31.0	33.0	30.0	33.0
2	31.168141813163192	31.0	31.0	33.0	30.0	34.0
3	31.29788683001878	31.0	31.0	34.0	28.0	34.0
4	35.203365234507565	37.0	35.0	37.0	33.0	37.0
5	35.08449964931333	37.0	35.0	37.0	32.0	37.0
6	35.171848684359375	37.0	35.0	37.0	32.0	37.0
7	35.49398447928686	35.0	35.0	37.0	35.0	37.0
8	35.55235095816647	37.0	35.0	37.0	35.0	37.0
9	37.37523699631213	39.0	37.0	39.0	35.0	39.0
10	36.82064028597932	39.0	37.0	39.0	32.0	39.0
11	36.511721984660284	38.0	35.0	39.0	32.0	39.0
12	35.640959297721665	37.0	35.0	39.0	31.0	39.0
13	35.32489151338265	37.0	35.0	39.0	30.0	39.0
14	36.12043666146293	38.0	35.0	40.0	31.0	41.0
15	36.41981990542772	38.0	35.0	40.0	31.0	41.0
16	36.508901558858796	38.0	35.0	40.0	32.0	41.0
17	36.34001085997421	37.0	35.0	40.0	31.0	41.0
18	36.41859046584764	37.0	35.0	40.0	31.0	41.0
19	36.294474535622975	37.0	35.0	40.0	31.0	41.0
20	36.04364849883481	36.0	34.0	40.0	31.0	41.0
21	35.96314260503631	36.0	34.0	40.0	31.0	41.0
22	35.81082196429784	36.0	34.0	40.0	30.0	41.0
23	35.67634516618023	36.0	34.0	40.0	30.0	41.0
24	35.7305889273513	36.0	34.0	40.0	30.0	41.0
25	35.59188035928415	36.0	34.0	40.0	30.0	41.0
26	35.33953211611122	35.0	34.0	40.0	29.0	41.0
27	35.36212402995543	36.0	34.0	40.0	29.0	41.0
28	35.39567003778366	36.0	34.0	40.0	29.0	41.0
29	35.332470870381684	36.0	34.0	40.0	29.0	41.0
30	35.290872191678545	36.0	34.0	40.0	29.0	41.0
31	35.123222697346094	36.0	34.0	40.0	29.0	41.0
32	34.99198217154234	36.0	34.0	40.0	28.0	41.0
33	34.7384180637571	35.0	34.0	40.0	27.0	41.0
34	34.46724767528677	35.0	34.0	40.0	25.0	41.0
35	34.23829407905156	35.0	33.0	40.0	24.0	41.0
36	34.02105477499491	35.0	33.0	40.0	23.0	41.0
37	34.043941265639496	35.0	33.0	40.0	23.0	41.0
38	34.05783253014774	35.0	33.0	40.0	23.0	41.0
39	34.03251023778819	35.0	33.0	40.0	23.0	41.0
40	33.78935134279056	35.0	33.0	40.0	23.0	41.0
41	33.790934184031315	35.0	33.0	40.0	23.0	41.0
42	33.76052489875337	35.0	33.0	40.0	23.0	41.0
43	33.718437068711964	35.0	33.0	40.0	23.0	41.0
44	33.664434941967016	35.0	33.0	40.0	23.0	41.0
45	33.72349057670988	35.0	33.0	40.0	23.0	41.0
46	33.70415439263332	35.0	33.0	40.0	23.0	41.0
47	33.62563134912555	35.0	33.0	40.0	23.0	41.0
48	33.65696870969931	35.0	33.0	40.0	23.0	41.0
49	33.64950247743162	36.0	33.0	40.0	23.0	41.0
50	33.48772641914975	35.0	33.0	39.0	23.0	41.0
51	33.21337541573339	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	5.0
13	7.0
14	10.0
15	51.0
16	173.0
17	511.0
18	1184.0
19	2471.0
20	4409.0
21	7285.0
22	11357.0
23	16638.0
24	24033.0
25	33628.0
26	43101.0
27	49223.0
28	52693.0
29	58414.0
30	69121.0
31	84262.0
32	106180.0
33	138016.0
34	233265.0
35	330281.0
36	151200.0
37	184971.0
38	261819.0
39	345548.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.16692685354872	23.915654200321274	29.00106337247449	14.916355573655512
2	32.615127039073286	25.831127401072422	29.67736826625037	11.876377293603928
3	27.1757279576461	26.49154958256974	33.76813955066857	12.56458290911559
4	25.20400914047829	27.417679133012058	33.571800266974364	13.806511459535283
5	21.66211905246725	31.932577660128057	32.67834113893979	13.726962148464898
6	19.76338831195276	40.39824430416978	30.15226588836851	9.686101495508947
7	85.0323762981063	4.71816104436752	8.748387972578566	1.5010746849476233
8	87.03536279101337	3.2096201271521982	8.292359555646055	1.4626575261883754
9	81.78718975542432	5.635421615873662	10.073214326115975	2.5041743025860312
10	47.62311364510509	25.777325278852466	17.6419376003982	8.957623475644246
11	42.90988483902351	20.158510373537865	24.660376931604787	12.271227855833843
12	41.75067309215141	21.26921423561619	26.22941695513473	10.750695717097672
13	17.843028122808207	44.83567501527184	23.552569062648477	13.768727799271478
14	11.671259530758613	45.1221520848888	27.5527500622186	15.653838322133984
15	11.35668227787959	23.83072015203964	50.249688906988844	14.562908663091925
16	11.438856082716804	19.927735921627185	48.330188465802394	20.303219529853617
17	11.099798637978235	20.857575963257087	33.23210932374035	34.810516075024324
18	15.309124640828978	24.031041426276612	42.65657594063214	18.003257992262267
19	27.532749609719676	23.39632118373719	28.14660965180208	20.924319554741057
20	28.510102038507657	21.6988167153103	32.39344781556144	17.3976334306206
21	15.484467974388561	32.859114459603155	31.456413041019026	20.200004524989254
22	18.77114866852191	26.804362089640037	24.043168397475057	30.381320844362996
23	14.085567546777074	33.62062490101586	27.56284078825313	24.730966763953933
24	12.495033824294667	22.451050928754043	51.2218375981357	13.832077648815583
25	12.563270662232176	27.83556189054051	42.404986538156976	17.196180909070343
26	12.541550713817054	36.58508111043236	30.371411117898596	20.50195705785199
27	11.055227493834701	42.84069775334284	27.781669268535484	18.322405484286975
28	10.472227878458789	31.978596800832598	40.50449105183375	17.04468426887486
29	11.249213783117264	23.415823887418266	40.6856716215299	24.64929070793457
30	14.583090115160976	39.04477476865992	31.319260616756033	15.052874499423064
31	26.658793185366186	31.707685694246475	27.814113441480576	13.819407678906762
32	29.686553994434263	23.193601665196045	29.766329554967307	17.353514785402385
33	29.097490893459128	29.897780492771332	23.44347157175502	17.561257042014525
34	17.64374759609946	27.106359872395302	27.750356342903686	27.499536188601553
35	19.04346252177651	25.210072626077512	35.171248218285484	20.575216633860492
36	31.023824068417838	29.505282924953054	24.981062919975567	14.489830086653544
37	15.335867327315098	41.32659109934614	30.390054073621574	12.94748749971719
38	15.637322111359985	37.77216679110387	29.184913685829994	17.405597411706147
39	18.073757324826353	37.54383583338989	29.081789180750693	15.300617661033055
40	24.809113328355846	26.844543994208014	27.66180230321953	20.684540374216613
41	14.182764315934747	24.73503925428177	31.925428177108078	29.156768252675402
42	14.719201791895745	24.033168171225594	28.95721622661146	32.2904138102672
43	15.620715400800922	24.65055770492545	30.404398289554063	29.32432860471956
44	13.610534174981334	29.533292608430056	33.6666440417204	23.18952917486821
45	12.329600217199484	46.44625444014571	22.972239190931923	18.251906151722892
46	16.977533428358107	40.33584470236883	26.08678929387543	16.599832575397635
47	16.070227833208897	30.776985904658478	29.119029842304123	24.033756419828503
48	16.212176746080228	27.1312020633951	38.7177085454422	17.938912645082468
49	20.240684178375076	28.001040747528222	36.86771193918415	14.890563134912554
50	17.693703477454243	38.857575963257084	28.489287087943165	14.959433471345507
51	15.335098079142062	34.982601416321636	27.550713817054685	22.131586687481615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2062.0
1	3835.0
2	5608.0
3	16388.5
4	27169.0
5	20131.0
6	13093.0
7	13208.0
8	13323.0
9	13901.5
10	14480.0
11	14517.0
12	14554.0
13	14259.5
14	13965.0
15	13590.0
16	13215.0
17	12806.5
18	12398.0
19	11200.0
20	10002.0
21	9880.5
22	9759.0
23	11250.5
24	12742.0
25	14996.0
26	24472.0
27	31694.0
28	39251.0
29	46808.0
30	54055.0
31	61302.0
32	74434.0
33	87566.0
34	104673.0
35	121780.0
36	121485.5
37	121191.0
38	129906.0
39	138621.0
40	179627.5
41	220634.0
42	249168.0
43	277702.0
44	263686.5
45	249671.0
46	219726.5
47	189782.0
48	161126.0
49	132470.0
50	115775.0
51	99080.0
52	81379.5
53	63679.0
54	54818.5
55	45958.0
56	41772.5
57	37587.0
58	33455.5
59	29324.0
60	25009.5
61	20695.0
62	17950.0
63	15205.0
64	13456.0
65	11707.0
66	9786.0
67	7865.0
68	7319.5
69	6774.0
70	5883.5
71	4993.0
72	4478.0
73	3963.0
74	3172.0
75	1631.5
76	882.0
77	716.0
78	550.0
79	388.5
80	227.0
81	178.0
82	129.0
83	100.0
84	71.0
85	46.0
86	21.0
87	13.5
88	6.0
89	6.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2209950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.876381216828282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33224161075168	14.975056354300762
2	6.974936221822374	2.633231101723662
3	2.7198630835376587	1.5402351726730474
4	1.626225173460302	1.2278898527455744
5	1.149471240106311	1.084892866301354
6	0.8551621398524115	0.9685419932431647
7	0.6950831891545508	0.9184458679123044
8	0.56824611064679	0.8581144167639035
9	0.4960573823750645	0.8427391429620306
>10	3.871334012775334	14.443341211539654
>50	0.5658203598891385	7.736826789670209
>100	1.1080316978189984	47.060573285741846
>500	0.034641025670202	4.180582805564453
>1k	0.002646189460918208	0.8598294167466427
>5k	0.0	0.0
>10k+	2.405626782652916E-4	0.6696997221114067
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14748	0.6673454150546393	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3255	0.14728840019004955	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2925	0.1323559356546528	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021131699812212947	0.0
2	0.0	0.0	0.0	0.07398357428901106	0.0
3	0.0	0.0	0.0	0.10778524401004548	0.0
4	0.0	0.0	0.0	0.1614516165524107	0.0
5	0.0	0.0	0.0	0.29579854747844975	0.0
6	0.0	0.0	0.0	0.4391954569107898	0.0
7	0.0	0.0	0.0	0.5463472024253943	0.0
8	0.0	0.0	0.0	0.8073938324396479	0.0
9	0.0	0.0	0.0	0.9219665603294193	0.0
10	0.0	0.0	0.0	1.1170388470327384	0.0
11	0.0	0.0	0.0	1.2618837530260865	0.0
12	0.0	0.0	0.0	1.4027014185841309	0.0
13	0.0	0.0	0.0	1.4615715287676192	0.0
14	0.0	0.0	0.0	1.486911468585262	0.0
15	0.0	0.0	0.0	1.5228851331478088	0.0
16	0.0	0.0	0.0	1.596687707866694	0.0
17	0.0	0.0	0.0	1.6897214869114685	0.0
18	0.0	0.0	0.0	1.83135365053508	0.0
19	0.0	0.0	0.0	1.887508767166678	0.0
20	0.0	0.0	0.0	1.9468766261680128	0.0
21	0.0	0.0	0.0	2.028507432294848	0.0
22	0.0	0.0	0.0	2.122898708115568	0.0
23	0.0	0.0	0.0	2.2314532002986494	0.0
24	0.0	0.0	0.0	2.31715649675332	0.0
25	0.0	0.0	0.0	2.3913210706124572	0.0
26	0.0	0.0	0.0	2.467431389850449	0.0
27	0.0	0.0	0.0	2.5474331998461506	0.0
28	0.0	0.0	0.0	2.6270730107015994	0.0
29	0.0	0.0	0.0	2.7110568112400735	0.0
30	0.0	0.0	0.0	2.8363537636598113	0.0
31	0.0	0.0	0.0	2.9436865087445416	0.0
32	0.0	0.0	0.0	3.0488020090952284	0.0
33	0.0	0.0	0.0	3.161066992465893	0.0
34	0.0	0.0	0.0	3.286454444670694	0.0
35	0.0	0.0	0.0	3.447317812620195	0.0
36	0.0	0.0	0.0	3.5714835177266453	0.0
37	0.0	0.0	0.0	3.686915993574515	0.0
38	0.0	0.0	0.0	3.81212244620919	0.0
39	0.0	4.524989253150524E-5	0.0	3.936650150455893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	80	0.0	45.000004	1
TGTATCG	65	0.0	45.000004	1
TACGGAC	20	7.034701E-4	45.000004	41
CGTTAGT	20	7.034701E-4	45.000004	25
CCCGTAG	20	7.034701E-4	45.000004	24
CGTAGAT	20	7.034701E-4	45.000004	41
CACTACG	40	6.8193913E-9	45.000004	1
TATTACG	65	0.0	45.000004	1
ACGTCCA	20	7.034701E-4	45.000004	14
ACCGTAA	20	7.034701E-4	45.000004	24
CCACGTA	20	7.034701E-4	45.000004	10
TCGAACA	20	7.034701E-4	45.000004	44
ATTCGAT	35	1.2126111E-7	45.0	14
GCCGATA	45	3.8562575E-10	45.0	9
GTGTCGA	25	3.891979E-5	45.0	8
GACGGTT	50	2.1827873E-11	45.0	28
TCGATTG	60	0.0	44.999996	1
TCCGTAA	30	2.1664146E-6	44.999996	12
TACGAAG	115	0.0	43.04348	1
TCGCGCA	105	0.0	42.857143	34
>>END_MODULE