##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545137_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1684980 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93612802525846 31.0 31.0 33.0 30.0 33.0 2 31.25616624529668 31.0 31.0 33.0 30.0 34.0 3 31.431446070576506 31.0 31.0 34.0 30.0 34.0 4 35.27068273807404 37.0 35.0 37.0 33.0 37.0 5 35.15817101686667 37.0 35.0 37.0 32.0 37.0 6 35.22062754454059 37.0 35.0 37.0 32.0 37.0 7 35.586176097045666 36.0 35.0 37.0 35.0 37.0 8 35.65408372799677 37.0 35.0 37.0 35.0 37.0 9 37.49499578630014 39.0 37.0 39.0 35.0 39.0 10 37.04115538463365 39.0 37.0 39.0 33.0 39.0 11 36.646953079561776 39.0 35.0 39.0 32.0 39.0 12 35.02014267231659 35.0 35.0 39.0 31.0 39.0 13 34.4589532219967 35.0 33.0 39.0 27.0 39.0 14 34.99468361642275 35.0 33.0 40.0 27.0 41.0 15 35.497532908402476 35.0 33.0 40.0 30.0 41.0 16 35.78198851024938 35.0 34.0 40.0 32.0 41.0 17 35.65951583995062 35.0 34.0 40.0 31.0 41.0 18 35.780706002445136 36.0 35.0 39.0 31.0 41.0 19 35.64977803890847 36.0 35.0 39.0 31.0 41.0 20 35.36476872129046 35.0 34.0 39.0 31.0 41.0 21 35.19503020807369 35.0 34.0 39.0 30.0 41.0 22 35.01574143313274 35.0 34.0 39.0 30.0 41.0 23 34.99115241723938 35.0 34.0 39.0 30.0 41.0 24 35.1033852033852 35.0 34.0 39.0 30.0 41.0 25 35.01364526581918 35.0 34.0 39.0 30.0 41.0 26 34.79813291552422 35.0 34.0 39.0 29.0 41.0 27 34.76239005804223 35.0 34.0 39.0 29.0 41.0 28 34.90525822264953 35.0 34.0 39.0 29.0 41.0 29 34.91842573799096 35.0 34.0 39.0 29.0 41.0 30 34.88156773374165 35.0 34.0 38.0 29.0 41.0 31 34.625764697503826 35.0 34.0 39.0 29.0 41.0 32 34.46660079051384 35.0 34.0 39.0 29.0 41.0 33 34.22282104238626 35.0 33.0 39.0 27.0 41.0 34 34.04822609170435 35.0 34.0 39.0 27.0 41.0 35 33.84198862894515 35.0 33.0 38.0 25.0 41.0 36 33.545343564908784 35.0 33.0 38.0 23.0 41.0 37 33.548302056997706 35.0 33.0 38.0 23.0 41.0 38 33.644349487827746 35.0 33.0 38.0 24.0 41.0 39 33.64743261047609 35.0 33.0 38.0 24.0 41.0 40 33.29227646618951 35.0 32.0 38.0 23.0 41.0 41 33.449899702073616 35.0 33.0 38.0 23.0 41.0 42 33.45523329653764 35.0 33.0 38.0 23.0 41.0 43 33.41753789362485 35.0 33.0 38.0 23.0 41.0 44 33.411474913648824 35.0 33.0 38.0 23.0 41.0 45 33.475960545525766 35.0 33.0 38.0 23.0 41.0 46 33.49829256133604 35.0 33.0 38.0 23.0 41.0 47 33.46112891547674 35.0 33.0 38.0 23.0 41.0 48 33.5271599662904 35.0 33.0 38.0 23.0 41.0 49 33.6065923631141 35.0 33.0 38.0 24.0 41.0 50 33.35206233901886 35.0 33.0 38.0 24.0 40.0 51 32.990068724851334 35.0 32.0 38.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 3.0 12 1.0 13 6.0 14 17.0 15 31.0 16 104.0 17 312.0 18 829.0 19 1781.0 20 3120.0 21 5047.0 22 7961.0 23 12249.0 24 17199.0 25 24221.0 26 31011.0 27 36569.0 28 40362.0 29 46190.0 30 55621.0 31 69614.0 32 89879.0 33 121788.0 34 234087.0 35 352785.0 36 85456.0 37 100779.0 38 145144.0 39 202698.0 40 114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.266175266175267 24.379043074695247 31.813196595805294 13.541585063324193 2 29.393761350283086 26.703403007750833 31.416812068985983 12.486023572980095 3 28.350781611651175 26.079063252976297 33.03321107668934 12.536944058683188 4 25.303089650915737 28.145972059015538 32.82050825529087 13.73043003477786 5 23.1510166292775 32.26625835321488 32.07254685515555 12.510178162352076 6 19.221533786751177 42.47593443245617 28.8631912544956 9.439340526297048 7 87.46833790312051 4.3283006326484585 6.812543769065509 1.3908176951655213 8 90.36789754181058 2.603829125568256 5.886241973198495 1.1420313594226639 9 86.24980711937233 4.330733895951287 7.8614582962409045 1.558000688435471 10 65.22492848579805 16.257403648707996 11.631354674832936 6.886313190661017 11 62.29676316632838 14.510973424016901 15.947548338852688 7.244715070802027 12 55.472290472290474 20.417274982492373 17.44750679533288 6.662927749884272 13 17.725551638595118 57.50400598226685 17.031418770549205 7.739023608588826 14 9.418153331196809 57.27812793030185 24.55483151135325 8.748887227148098 15 7.518071431114909 21.631948153687283 62.08874882787926 8.761231587318544 16 7.821220429916082 14.04123491080013 61.43360752056404 16.703937138719745 17 8.982895939417679 15.106766845897281 30.164690382081687 45.745646832603356 18 14.27856710465406 20.75086944652162 47.54234471625776 17.42821873256656 19 34.685752946622515 16.78963548528766 28.534285273415712 19.99032629467412 20 35.48914527175397 18.419743854526462 28.779273344490736 17.311837529228832 21 16.106125888734585 34.585514368123064 32.692910301605956 16.6154494415364 22 19.49732341036689 23.441821267908225 20.07881399185747 36.982041329867414 23 11.579603318733755 35.284038979691154 24.1856283160631 28.95072938551199 24 9.723023418675593 20.00979240109675 58.125497038540516 12.141687141687143 25 8.514403731795037 22.758727106553195 52.97053970967014 15.756329451981626 26 8.497252192904368 42.674631152892026 29.989317380621728 18.838799273581884 27 8.497311540789802 54.74492278840105 24.545810632767154 12.211955038041994 28 7.486795095490748 36.87717361630405 43.91707913447044 11.718952153734763 29 8.441583876366485 24.663141402271837 43.243005851701504 23.652268869660173 30 12.773979513109948 45.63917672613324 29.25518403779273 12.331659722964071 31 33.91642630773066 30.70244157200679 25.89508480812829 9.486047312134268 32 38.251729990860426 21.013543187456232 30.185462141983884 10.549264679699462 33 39.00141247967335 27.10079644862254 19.560232168927822 14.337558902776292 34 19.45797576232359 26.50227302401215 23.384075775380122 30.655675438284135 35 17.627865019169366 23.924319576493488 36.44891927500623 21.998896129330912 36 42.12584125627604 23.858324727889947 21.89112036938124 12.124713646452777 37 16.01413666631058 46.056807795938234 27.800033234815842 10.129022302935347 38 16.301795867013258 44.15423328466807 21.118885684103077 18.4250851642156 39 18.841173188999278 41.57693266388919 26.118410901019594 13.463483246091942 40 29.349843915061307 23.160809030374246 24.458213153865326 23.031133900699118 41 12.674453109235717 22.989412337238424 27.7890538760104 36.54708067751546 42 16.058172797303232 18.960403090837875 26.607081389690084 38.374342722168805 43 15.853066505240418 19.322128452563238 30.076024641242032 34.74878040095432 44 10.98054576315446 30.076855511638122 36.36161853553158 22.58098018967584 45 10.212584125627604 55.13276121971774 21.238352977483412 13.41630167717124 46 18.619924272098185 44.292869945043854 22.96632600980427 14.120879773053685 47 17.29070968201403 31.06268323659628 23.626808626808625 28.019798454581064 48 18.215705824401475 26.716281498890194 40.357927097057534 14.710085579650798 49 22.840745884224145 25.668435233652627 39.39643200512766 12.094386876995571 50 19.60658286745243 45.66546783938089 23.161165117686856 11.566784175479828 51 16.16131942218899 40.831048439744094 22.788163657728873 20.219468480338048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2246.0 1 2653.5 2 3061.0 3 6923.0 4 10785.0 5 8736.0 6 6687.0 7 7312.0 8 7937.0 9 8468.5 10 9000.0 11 9224.5 12 9449.0 13 9089.0 14 8729.0 15 8083.0 16 7437.0 17 6808.5 18 6180.0 19 6175.5 20 6171.0 21 6489.5 22 6808.0 23 6744.5 24 6681.0 25 7876.0 26 10759.0 27 12447.0 28 16803.0 29 21159.0 30 28405.5 31 35652.0 32 43238.5 33 50825.0 34 56734.0 35 62643.0 36 64814.0 37 66985.0 38 78576.0 39 90167.0 40 145978.5 41 201790.0 42 244472.5 43 287155.0 44 275611.0 45 264067.0 46 224200.5 47 184334.0 48 150961.0 49 117588.0 50 100721.0 51 83854.0 52 63059.5 53 42265.0 54 30780.5 55 19296.0 56 15344.5 57 11393.0 58 10338.5 59 9284.0 60 8329.0 61 7374.0 62 6231.0 63 5088.0 64 4494.0 65 3900.0 66 3194.0 67 2488.0 68 2091.0 69 1694.0 70 1506.0 71 1318.0 72 1122.5 73 927.0 74 732.5 75 398.0 76 258.0 77 187.5 78 117.0 79 96.0 80 75.0 81 52.0 82 29.0 83 21.0 84 13.0 85 11.0 86 9.0 87 4.5 88 0.0 89 1.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1684980.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.910444728047914 #Duplication Level Percentage of deduplicated Percentage of total 1 80.59777320646215 12.01748642598695 2 7.707304791925002 2.298386842044332 3 2.683362119859859 1.2003036772052376 4 1.4228178089435235 0.8485938519333858 5 0.9057331882550168 0.6752442320917522 6 0.6463043536570525 0.5782011205620129 7 0.4587815011859008 0.47884453509782565 8 0.4092332434655882 0.4881479726058743 9 0.32199494839896053 0.4320979092732008 >10 3.0160512934835024 9.527863884236796 >50 0.47783711907848464 5.094027511147856 >100 1.144651626828557 45.994402170053746 >500 0.1953698588199526 17.669891170248732 >1k 0.012385410272839533 2.382149338255206 >5k 3.9952936363998496E-4 0.31435935925709524 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5277 0.313178791439661 No Hit TTGATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 2239 0.13287991548861114 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 2181 0.1294377381333903 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1815 0.10771641206423815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.028012201925245405 0.0 2 0.0 0.0 0.0 0.0954907476646607 0.0 3 0.0 0.0 0.0 0.13216774086339303 0.0 4 0.0 0.0 0.0 0.1978658500397631 0.0 5 0.0 0.0 0.0 0.33804555543685977 0.0 6 0.0 0.0 0.0 0.4738335173117782 0.0 7 0.0 0.0 0.0 0.5588790371399067 0.0 8 0.0 0.0 0.0 0.7546083633040155 0.0 9 0.0 0.0 0.0 0.8303956130043086 0.0 10 0.0 0.0 0.0 0.971465536682928 0.0 11 0.0 0.0 0.0 1.10398936485893 0.0 12 0.0 0.0 0.0 1.224168832864485 0.0 13 0.0 0.0 0.0 1.276394972047146 0.0 14 0.0 0.0 0.0 1.2933684672815107 0.0 15 0.0 0.0 0.0 1.3260098042706738 0.0 16 0.0 0.0 0.0 1.398414224501181 0.0 17 0.0 0.0 0.0 1.4936675806241024 0.0 18 0.0 0.0 0.0 1.6603757908105734 0.0 19 0.0 0.0 0.0 1.7146791059834539 0.0 20 0.0 0.0 0.0 1.7700506830941614 0.0 21 0.0 0.0 0.0 1.8511792424835902 0.0 22 0.0 0.0 0.0 1.9315956272478012 0.0 23 0.0 0.0 0.0 2.035691818300514 0.0 24 0.0 0.0 0.0 2.1091051525834135 0.0 25 0.0 0.0 0.0 2.1680969507056465 0.0 26 0.0 0.0 0.0 2.2368218020391932 0.0 27 0.0 0.0 0.0 2.296941209984688 0.0 28 0.0 0.0 0.0 2.3677432373084546 0.0 29 0.0 0.0 0.0 2.442343529300051 0.0 30 0.0 0.0 0.0 2.545252762644067 0.0 31 0.0 0.0 0.0 2.6485774311861268 0.0 32 0.0 0.0 0.0 2.7381333903073033 0.0 33 0.0 0.0 0.0 2.8316656577526143 0.0 34 0.0 0.0 0.0 2.9251385773124903 0.0 35 0.0 0.0 0.0 3.0731522035869863 0.0 36 0.0 0.0 0.0 3.183717314152097 0.0 37 0.0 0.0 0.0 3.289119158684376 0.0 38 0.0 0.0 0.0 3.390485347007086 0.0 39 0.0 0.0 0.0 3.4868069650678346 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 45 3.8562575E-10 45.000004 25 GCAAGCG 45 3.8562575E-10 45.000004 1 CGAACAT 45 3.8562575E-10 45.000004 41 CCGATAG 90 0.0 45.000004 11 GCGCGAC 90 0.0 45.000004 9 CACGACG 45 3.8562575E-10 45.000004 26 AAGCGCG 90 0.0 45.000004 1 TCGTCAG 45 3.8562575E-10 45.000004 1 TCGTAAA 45 3.8562575E-10 45.000004 15 ATACGCA 45 3.8562575E-10 45.000004 36 ACGACGG 45 3.8562575E-10 45.000004 27 CGGAACC 25 3.8915823E-5 45.0 35 AACCGAT 40 6.8175723E-9 45.0 15 AGCCGAA 25 3.8915823E-5 45.0 14 TCGTTAG 25 3.8915823E-5 45.0 1 TCACCGC 25 3.8915823E-5 45.0 12 GCGACAG 20 7.034225E-4 45.0 1 CTCCGTA 35 1.2123746E-7 45.0 39 GTACACG 40 6.8175723E-9 45.0 1 CATGCGT 20 7.034225E-4 45.0 39 >>END_MODULE