FastQCFastQC Report
Thu 26 May 2016
SRR1545136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545136_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1456905
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.3784049062910759No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23010.1579375456876049No Hit
TTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA17810.12224544496724221No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16110.11057687357789286No Hit
TGGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA14650.10055562991409872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.2122291E-745.00000425
TAACGCG1300.045.0000041
GCGCATG1300.045.0000041
CTCGATT351.2122291E-745.00000441
CGTAAGG1250.045.0000042
GGTTGAC351.2122291E-745.00000423
TTACGAG351.2122291E-745.0000041
TACTACC351.2122291E-745.00000427
ATACGTC351.2122291E-745.00000429
TCGAAAG351.2122291E-745.0000041
TGCGTTG551.8189894E-1245.0000041
ATCGACG351.2122291E-745.0000041
ATTTCGA850.045.0000041
ACGCGAG406.8175723E-945.01
CTTAACG207.0339086E-445.01
CTTCGCA207.0339086E-445.029
AGGTACG207.0339086E-445.01
ATCTCGG207.0339086E-445.011
CTCACGT253.891321E-545.045
CGTATGG1650.045.02