##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545136_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1456905
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.059318898624138	31.0	31.0	33.0	30.0	33.0
2	31.383917276692713	31.0	31.0	33.0	30.0	34.0
3	31.53587364996345	31.0	31.0	34.0	30.0	34.0
4	35.39873361681098	37.0	35.0	37.0	33.0	37.0
5	35.30018978588171	37.0	35.0	37.0	33.0	37.0
6	35.38696414659844	37.0	35.0	37.0	33.0	37.0
7	35.687479279705954	37.0	35.0	37.0	35.0	37.0
8	35.756534571574676	37.0	35.0	37.0	35.0	37.0
9	37.632831927956865	39.0	37.0	39.0	35.0	39.0
10	37.16865821724821	39.0	37.0	39.0	33.0	39.0
11	36.77757575133588	39.0	35.0	39.0	33.0	39.0
12	35.299522618152864	35.0	35.0	39.0	31.0	39.0
13	34.81179967122084	35.0	34.0	39.0	30.0	39.0
14	35.437822644578745	35.0	34.0	40.0	30.0	41.0
15	35.88365473383646	35.0	34.0	40.0	31.0	41.0
16	36.10391274654147	35.0	35.0	40.0	32.0	41.0
17	35.982500574848736	35.0	35.0	40.0	32.0	41.0
18	36.10228738318559	36.0	35.0	40.0	32.0	41.0
19	35.9736228511811	36.0	35.0	40.0	31.0	41.0
20	35.715428940116205	35.0	34.0	40.0	31.0	41.0
21	35.55912018971724	35.0	34.0	40.0	31.0	41.0
22	35.386640172145746	35.0	34.0	40.0	30.0	41.0
23	35.34176353296886	35.0	34.0	39.0	30.0	41.0
24	35.43760711920132	35.0	34.0	39.0	31.0	41.0
25	35.32186518681726	35.0	34.0	39.0	31.0	41.0
26	35.10396010721358	35.0	34.0	39.0	30.0	41.0
27	35.103389033602056	35.0	34.0	39.0	30.0	41.0
28	35.23095671989594	35.0	34.0	39.0	30.0	41.0
29	35.249734196807616	36.0	34.0	39.0	30.0	41.0
30	35.21785222783915	36.0	34.0	39.0	30.0	41.0
31	34.959080379297205	35.0	34.0	39.0	30.0	41.0
32	34.810474258788325	35.0	34.0	39.0	29.0	41.0
33	34.58584121819885	35.0	34.0	39.0	29.0	41.0
34	34.39541081951123	35.0	34.0	39.0	27.0	41.0
35	34.175030629999895	35.0	33.0	39.0	27.0	41.0
36	33.89992346789942	35.0	33.0	39.0	24.0	41.0
37	33.89174723128824	35.0	33.0	39.0	24.0	41.0
38	33.959147645179335	35.0	33.0	39.0	26.0	41.0
39	33.97012159337774	35.0	33.0	39.0	25.0	41.0
40	33.64640934034821	35.0	33.0	39.0	23.0	41.0
41	33.77828822057718	35.0	33.0	39.0	23.0	41.0
42	33.76490162364739	35.0	33.0	39.0	24.0	41.0
43	33.751844492262705	35.0	33.0	39.0	24.0	41.0
44	33.70041972537674	35.0	33.0	39.0	23.0	41.0
45	33.73348433837484	35.0	33.0	39.0	24.0	41.0
46	33.756659493927195	35.0	33.0	39.0	24.0	41.0
47	33.74917513496076	35.0	33.0	39.0	24.0	41.0
48	33.803651576458314	35.0	33.0	39.0	24.0	41.0
49	33.856200644516974	35.0	33.0	39.0	24.0	41.0
50	33.63102810409738	35.0	33.0	39.0	24.0	41.0
51	33.36299690096472	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	5.0
14	11.0
15	22.0
16	60.0
17	239.0
18	533.0
19	1155.0
20	2254.0
21	3752.0
22	5975.0
23	8857.0
24	13561.0
25	19256.0
26	24780.0
27	28851.0
28	31559.0
29	36202.0
30	44048.0
31	55015.0
32	72488.0
33	100145.0
34	196117.0
35	298681.0
36	78587.0
37	94672.0
38	138805.0
39	201173.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.757321170563625	24.293896993970094	30.264430419279226	13.684351416187054
2	29.91080406752671	25.18434626828791	32.60240029377344	12.302449370411935
3	27.776004612517628	25.96456186230399	34.52634179991146	11.733091725266918
4	25.05008905865516	27.849653889580996	33.30093588806408	13.79932116369976
5	22.2504555890741	32.46910402531394	33.07566382159441	12.204776564017559
6	19.643696740693457	41.85894069963381	29.495128371444945	9.002234188227783
7	87.25036979075506	4.75233457226106	6.937240245589109	1.060055391394772
8	90.04917959647335	2.7080695035022875	6.3052155082177626	0.9375353918066037
9	86.95700817829577	3.9920241882621035	7.587111033320635	1.4638566001214903
10	60.89401848438985	21.203510180828538	12.084796194672954	5.817675140108655
11	59.24772033866312	13.94716882706834	18.73368544963467	8.071425384633864
12	53.549133265381066	20.070972369509338	18.549665214959106	7.83022915015049
13	17.757781049553678	54.92760337839461	18.80905069307882	8.50556487897289
14	9.318864304810539	54.80933897543079	25.535776183073022	10.336020536685647
15	7.777583301587955	22.06931817791826	60.880359391998795	9.272739128494994
16	8.259975770554703	16.04421702170011	59.18011126326013	16.515695944485056
17	9.116998019774798	15.30758697375601	31.99137898490293	43.58403602156626
18	15.386795981893123	21.065821038434215	47.35772064753707	16.189662332135587
19	32.4877737395369	18.400650694451596	29.461632707691994	19.64994285831952
20	34.12961037267358	18.18375254392016	31.121452668499316	16.565184414906945
21	16.241347239524885	33.14045871213291	33.34925750134703	17.26893654699517
22	20.471273006819253	24.239260624405848	21.308184130056524	33.98128223871838
23	12.121037404635167	33.20765595560452	25.65054001462003	29.020766625140283
24	9.88513321047014	18.970076978251843	59.2201962379153	11.92459357336271
25	9.31838383422392	23.653978811247132	51.57536009554501	15.452277258983942
26	9.284064506608185	40.95421458502785	30.384067595347673	19.377653313016292
27	8.59493240808426	52.46052419341001	26.91630545574351	12.028237942762225
28	7.611340478617342	36.71481668331154	43.75130842436535	11.922534413705767
29	8.825764205627683	24.342904993805362	42.77609041083667	24.055240389730283
30	13.309584358623248	43.83092926443385	30.492036200026767	12.367450176916133
31	31.99707599328714	31.545914112450706	26.380443474351452	10.076566419910701
32	35.65709500619464	20.558718653584137	32.68490395736167	11.099282382859554
33	36.32357634849218	27.917468880949688	20.97858130763502	14.780373462923112
34	19.61802588363689	26.54558807883836	24.97074277320759	28.865643264317164
35	18.255411299981812	23.891949028934626	36.47499322193279	21.37764644915077
36	40.06033337794846	24.966830369859395	22.876851956716465	12.095984295475683
37	15.309577494757724	44.85481208452164	29.551823900666136	10.2837865200545
38	15.627374468479413	42.93608711618122	23.386150778533946	18.05038763680542
39	19.012221112563964	40.62172893908663	27.544692344387588	12.821357603961822
40	28.10588198956006	23.056685233422908	25.100195276974134	23.737237500042898
41	13.014987250369792	23.6194535676657	28.93284050778877	34.43271867417573
42	14.584410102237278	20.269475360438737	28.49671049244803	36.64940404487596
43	15.986217358029522	19.312103397270242	32.23182019417876	32.469859050521485
44	11.036203458701838	29.55710907711896	37.026779371338556	22.379908092840644
45	11.190091323730785	53.67542839100696	22.391027554988145	12.743452730274107
46	18.948112608577773	42.592756562713426	23.79448213850594	14.664648690202862
47	16.896228649088307	31.100655155964184	25.15853813392088	26.84457806102663
48	17.26172948819587	27.881364948297932	39.945432269090986	14.911473294415215
49	22.832854578713093	26.177890802763393	38.98483428912661	12.004420329396906
50	19.607730085352166	44.74251924456296	23.577583987974506	12.072166682110364
51	15.546312216651051	39.441212707760634	24.61251763155456	20.399957444033756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2128.0
1	2515.5
2	2903.0
3	6951.0
4	10999.0
5	8793.0
6	6587.0
7	7063.5
8	7540.0
9	7978.0
10	8416.0
11	8460.5
12	8505.0
13	8414.0
14	8323.0
15	8317.0
16	8311.0
17	7466.5
18	6622.0
19	6106.5
20	5591.0
21	5232.0
22	4873.0
23	6294.5
24	7716.0
25	9154.0
26	13696.5
27	16801.0
28	19946.0
29	23091.0
30	29598.5
31	36106.0
32	41755.0
33	47404.0
34	52053.5
35	56703.0
36	61333.5
37	65964.0
38	79770.5
39	93577.0
40	131462.5
41	169348.0
42	204607.0
43	239866.0
44	223049.0
45	206232.0
46	176332.5
47	146433.0
48	124363.5
49	102294.0
50	83117.5
51	63941.0
52	49160.0
53	34379.0
54	25042.5
55	15706.0
56	12685.0
57	9664.0
58	8866.5
59	8069.0
60	7220.0
61	6371.0
62	5732.5
63	5094.0
64	4466.0
65	3838.0
66	3085.5
67	2333.0
68	2029.0
69	1725.0
70	1383.5
71	1042.0
72	906.0
73	770.0
74	660.5
75	389.0
76	227.0
77	182.0
78	137.0
79	106.0
80	75.0
81	55.0
82	35.0
83	22.5
84	10.0
85	8.0
86	6.0
87	6.0
88	6.0
89	3.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1456905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.29503273506804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52638811133889	11.511273540883831
2	7.088701009392671	2.026664259567562
3	2.5480693233398113	1.0927420316509582
4	1.4133879848774926	0.8081771004470243
5	0.8981197809553109	0.6419325834384152
6	0.6805594310039282	0.583717160661626
7	0.5424479504000251	0.5428017845627247
8	0.4347574560077348	0.49718976523563774
9	0.36849532892934117	0.47408875107881365
>10	3.3685061271423753	9.904325237522425
>50	0.5449280503548966	5.600847432200562
>100	1.471932090038502	54.89042618604465
>500	0.1059983829160296	9.692367762131392
>1k	0.0072271624715474735	1.3537384409531603
>5k	4.818108314364982E-4	0.37970796362120435
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5513	0.3784049062910759	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2301	0.1579375456876049	No Hit
TTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1781	0.12224544496724221	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1611	0.11057687357789286	No Hit
TGGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1465	0.10055562991409872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012423596596895474	0.0
2	0.0	0.0	0.0	0.05333223511484963	0.0
3	0.0	0.0	0.0	0.07467885689183577	0.0
4	0.0	0.0	0.0	0.10817452064479152	0.0
5	0.0	0.0	0.0	0.17763683973903582	0.0
6	0.0	0.0	0.0	0.24229445296707747	0.0
7	0.0	0.0	0.0	0.28711549483322524	0.0
8	0.0	0.0	0.0	0.41341062045912397	0.0
9	0.0	0.0	0.0	0.4602908219822157	0.0
10	0.0	0.0	0.0	0.5399803007059486	0.0
11	0.0	0.0	0.0	0.6225526029494031	0.0
12	0.0	0.0	0.0	0.6902989556628607	0.0
13	0.0	0.0	0.0	0.713430182475865	0.0
14	0.0	0.0	0.0	0.7228336782425758	0.0
15	0.0	0.0	0.0	0.7375223504621098	0.0
16	0.0	0.0	0.0	0.7791173755323786	0.0
17	0.0	0.0	0.0	0.825654383779313	0.0
18	0.0	0.0	0.0	0.9020492070519355	0.0
19	0.0	0.0	0.0	0.9343780136659563	0.0
20	0.0	0.0	0.0	0.9706878622834021	0.0
21	0.0	0.0	0.0	1.0196272234634378	0.0
22	0.0	0.0	0.0	1.0694588871614827	0.0
23	0.0	0.0	0.0	1.1302040970413307	0.0
24	0.0	0.0	0.0	1.1812026178783106	0.0
25	0.0	0.0	0.0	1.2208071219468668	0.0
26	0.0	0.0	0.0	1.2658340797787089	0.0
27	0.0	0.0	0.0	1.3112042308867085	0.0
28	0.0	0.0	0.0	1.3628891382760029	0.0
29	0.0	0.0	0.0	1.4110734742484925	0.0
30	0.0	0.0	0.0	1.477790247133478	0.0
31	0.0	0.0	0.0	1.5494490031951293	0.0
32	0.0	0.0	0.0	1.6191858769102996	0.0
33	0.0	0.0	0.0	1.6818529691366286	0.0
34	0.0	0.0	0.0	1.750011153781475	0.0
35	0.0	0.0	0.0	1.8560578761140911	0.0
36	0.0	0.0	0.0	1.9349236909750465	0.0
37	0.0	0.0	0.0	2.0196237915306763	0.0
38	0.0	0.0	0.0	2.0953322282509843	0.0
39	0.0	0.0	0.0	2.1733057405939302	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2122291E-7	45.000004	25
TAACGCG	130	0.0	45.000004	1
GCGCATG	130	0.0	45.000004	1
CTCGATT	35	1.2122291E-7	45.000004	41
CGTAAGG	125	0.0	45.000004	2
GGTTGAC	35	1.2122291E-7	45.000004	23
TTACGAG	35	1.2122291E-7	45.000004	1
TACTACC	35	1.2122291E-7	45.000004	27
ATACGTC	35	1.2122291E-7	45.000004	29
TCGAAAG	35	1.2122291E-7	45.000004	1
TGCGTTG	55	1.8189894E-12	45.000004	1
ATCGACG	35	1.2122291E-7	45.000004	1
ATTTCGA	85	0.0	45.000004	1
ACGCGAG	40	6.8175723E-9	45.0	1
CTTAACG	20	7.0339086E-4	45.0	1
CTTCGCA	20	7.0339086E-4	45.0	29
AGGTACG	20	7.0339086E-4	45.0	1
ATCTCGG	20	7.0339086E-4	45.0	11
CTCACGT	25	3.891321E-5	45.0	45
CGTATGG	165	0.0	45.0	2
>>END_MODULE