FastQCFastQC Report
Thu 26 May 2016
SRR1545135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545135_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1545429
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65730.4253187949753758No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25730.16649098729220171No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18760.12139024180340863No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15980.10340170916942804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15690.10152520756372503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT207.0340413E-445.00000425
AACCGTC351.2122837E-745.00000429
GGTACGA207.0340413E-445.0000048
ACGCCTA406.8175723E-945.0000047
CAAGTCG351.2122837E-745.0000041
ATTCGTC406.8175723E-945.00000438
AAGCGTG351.2122837E-745.0000041
TAACGTG207.0340413E-445.0000041
TCGATTG302.165989E-645.0000041
CCATGCG207.0340413E-445.0000041
CTACGCT207.0340413E-445.00000437
ACGCTAG406.8175723E-945.0000041
CCGACAG207.0340413E-445.00000411
CAGTCGC207.0340413E-445.00000437
TAGGCCG207.0340413E-445.0000041
ATACGTT351.2122837E-745.00000437
TAAATCG207.0340413E-445.00000417
GTGCGTA207.0340413E-445.00000416
CGTCAAT207.0340413E-445.00000418
ATACCGG351.2122837E-745.0000042