##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545135_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1545429
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.82394208986631	31.0	31.0	33.0	28.0	33.0
2	31.13097140017432	31.0	31.0	33.0	30.0	34.0
3	31.26969016370212	31.0	31.0	34.0	28.0	34.0
4	35.143188719766485	35.0	35.0	37.0	32.0	37.0
5	35.01305009806339	35.0	35.0	37.0	32.0	37.0
6	35.091711104165896	37.0	35.0	37.0	32.0	37.0
7	35.516595068424365	36.0	35.0	37.0	35.0	37.0
8	35.58267380772588	37.0	35.0	37.0	35.0	37.0
9	37.38123524277078	39.0	37.0	39.0	35.0	39.0
10	36.852561327631356	39.0	37.0	39.0	32.0	39.0
11	36.48299857191757	38.0	35.0	39.0	32.0	39.0
12	35.33110417883966	37.0	35.0	39.0	31.0	39.0
13	34.90821577697843	35.0	34.0	39.0	30.0	39.0
14	35.59118988966818	37.0	34.0	40.0	29.0	41.0
15	35.98640312819288	37.0	34.0	40.0	31.0	41.0
16	36.14093368249205	36.0	34.0	40.0	31.0	41.0
17	35.971118699079675	36.0	34.0	40.0	31.0	41.0
18	36.0714513575195	36.0	35.0	40.0	31.0	41.0
19	35.949291102988234	36.0	35.0	40.0	31.0	41.0
20	35.69598150416486	36.0	34.0	40.0	30.0	41.0
21	35.55788069202791	35.0	34.0	40.0	30.0	41.0
22	35.38111035835357	35.0	34.0	40.0	30.0	41.0
23	35.27521354911808	35.0	34.0	40.0	29.0	41.0
24	35.39152688347378	35.0	34.0	40.0	30.0	41.0
25	35.2602973025613	35.0	34.0	40.0	30.0	41.0
26	35.00071889423584	35.0	34.0	39.0	29.0	41.0
27	35.01172425261853	35.0	34.0	39.0	29.0	41.0
28	35.05487990713258	36.0	34.0	40.0	29.0	41.0
29	35.0504707754287	36.0	34.0	40.0	29.0	41.0
30	35.014494357230255	36.0	34.0	40.0	29.0	41.0
31	34.80917790464654	35.0	34.0	40.0	29.0	41.0
32	34.66723867612165	35.0	34.0	40.0	27.0	41.0
33	34.41806126324794	35.0	33.0	40.0	26.0	41.0
34	34.175844377192355	35.0	33.0	40.0	25.0	41.0
35	33.96615955828447	35.0	33.0	40.0	24.0	41.0
36	33.72029643548814	35.0	33.0	40.0	23.0	41.0
37	33.760803634460075	35.0	33.0	40.0	23.0	41.0
38	33.79085936655777	35.0	33.0	40.0	23.0	41.0
39	33.7738886742775	35.0	33.0	40.0	23.0	41.0
40	33.48333763634564	35.0	32.0	40.0	23.0	41.0
41	33.53901602726492	35.0	33.0	39.0	23.0	41.0
42	33.52045030861981	35.0	33.0	39.0	23.0	41.0
43	33.464973803390514	35.0	33.0	39.0	23.0	41.0
44	33.41979993904605	35.0	33.0	39.0	23.0	41.0
45	33.483569934303034	35.0	33.0	39.0	23.0	41.0
46	33.48132719134946	35.0	33.0	39.0	23.0	41.0
47	33.414811033052956	35.0	33.0	39.0	23.0	41.0
48	33.451741878792234	35.0	33.0	39.0	23.0	41.0
49	33.47934715862068	35.0	33.0	39.0	23.0	41.0
50	33.31136661729526	35.0	32.0	39.0	23.0	41.0
51	32.990129601554	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	1.0
12	3.0
13	3.0
14	5.0
15	43.0
16	139.0
17	377.0
18	885.0
19	2021.0
20	3458.0
21	5573.0
22	8407.0
23	12459.0
24	17680.0
25	24086.0
26	31001.0
27	35564.0
28	38809.0
29	43662.0
30	52113.0
31	63015.0
32	79047.0
33	104087.0
34	179431.0
35	253516.0
36	95288.0
37	115308.0
38	162831.0
39	216526.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.028563589786394	24.443568743695117	28.77000496302321	13.757862703495274
2	30.93134657108156	25.996988538457604	30.970817811753243	12.100847078707595
3	27.93392643725464	25.618064628009442	34.006026805501904	12.441982129234018
4	25.43921461290037	28.80882913417569	33.05011100477602	12.701845248147926
5	22.011299127944408	33.504742048971515	31.854908895847046	12.629049927237032
6	20.385925202645996	41.30063561638872	28.859494677529668	9.453944503435615
7	85.14839568818755	5.198103568653106	8.17080564684628	1.4826950963130625
8	87.21300040312431	3.7664622574055486	7.552013065627731	1.468524273842409
9	82.1652110837832	5.872155886812012	9.508233636097161	2.454399393307619
10	54.23587884011495	22.925025996017933	15.062031319458868	7.777063844408253
11	50.885417576608184	18.81328744316303	20.92525764690581	9.37603733332298
12	46.933181660238034	21.201944573319125	22.716216662169533	9.148657104273312
13	18.661355520053007	48.461042208991806	21.617298497698698	11.260303773256487
14	11.343516913426628	49.383504515574636	26.761695296257543	12.511283274741189
15	10.085160819422956	24.200529432280614	54.595196544131106	11.119113204165316
16	10.47443784217845	17.670497965289897	53.387700114337186	18.46736407819447
17	10.830067249935132	19.076838858336423	31.762895610215676	38.33019828151277
18	15.62886421828502	23.335397485099605	43.45188293994742	17.583855356667954
19	30.436079561079804	20.436396625144216	29.463210538950673	19.664313274825307
20	31.160862129544615	20.394790055059143	31.73960110752419	16.704746707872054
21	17.080694098531865	33.562460650084866	31.837502725780347	17.519342525602923
22	20.198145628171854	25.13936259769941	22.381293479027505	32.281198295101234
23	13.411227562055583	34.11402270825771	26.237439571795274	26.237310157891436
24	11.526637587362472	21.19819157010772	53.98630412655645	13.288866715973363
25	11.077571341032167	26.404189386895162	45.94698300601321	16.571256266059457
26	10.927062970864402	39.05433378045837	30.366843122524557	19.651760126152674
27	10.812596372916516	48.005505267469424	27.26278593193217	13.919112427681894
28	9.985123871753409	33.976067486762574	42.15289088013749	13.885917761346525
29	10.019547970175271	26.02261249141824	40.12549266255518	23.8323468758513
30	14.03720261493734	41.907780946261525	30.62599446496733	13.429021973833802
31	29.38769752605911	31.818220054107954	26.761760003209467	12.032322416623476
32	32.48748405782472	23.119082144828393	30.80749746510516	13.585936332241728
33	32.349917078041116	29.045591871253873	23.22714275453612	15.377348296168897
34	18.659673139303067	26.68068219245271	26.86477347066737	27.794871197576853
35	18.93726596304327	24.91269414512087	34.75158030553329	21.39845958630257
36	34.51837645081074	27.047311782035926	25.389972622488642	13.044339144664685
37	16.087765921307287	42.06670122017899	29.343826212656808	12.501706645856911
38	15.396825088697055	40.49587525534981	26.173962051960974	17.93333760399216
39	18.52650623225007	38.464465206748415	28.51046537886891	14.498563182132598
40	25.563193132780604	25.693059985285636	27.253985786470942	21.489761095462814
41	14.095374164714134	24.97086569489766	29.792892458987115	31.140867681401087
42	15.566616130537216	22.411899867286042	28.435922970256154	33.585561031920584
43	16.86004339248196	22.411446918622595	30.50583365525042	30.222676033645023
44	12.54894272075909	30.805103307884092	34.34205000682658	22.303903964530235
45	12.307715203998372	49.17560107905313	23.378945263742303	15.137738453206198
46	17.67179210432831	41.60003468292623	24.788650918288706	15.939522294456749
47	17.09997677020426	30.892392986025243	27.099853826995613	24.907776416774887
48	17.0655526717824	27.92156740943777	38.49960108164141	16.513278837138426
49	21.138467053484824	28.427381652602612	37.08750126987393	13.346650024038635
50	18.339244313391298	41.339783322300796	26.358635692742922	13.962336671564982
51	16.34743491936543	37.08465416398942	25.46613270489942	21.101778211745735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2078.0
1	2791.0
2	3504.0
3	8679.0
4	13854.0
5	11050.0
6	8246.0
7	8637.0
8	9028.0
9	9442.0
10	9856.0
11	9713.0
12	9570.0
13	9325.0
14	9080.0
15	8345.5
16	7611.0
17	7343.5
18	7076.0
19	7190.0
20	7304.0
21	7284.0
22	7264.0
23	8219.5
24	9175.0
25	11625.5
26	18151.5
27	22227.0
28	25501.5
29	28776.0
30	35503.5
31	42231.0
32	47000.0
33	51769.0
34	63478.0
35	75187.0
36	74546.0
37	73905.0
38	85493.0
39	97081.0
40	130178.0
41	163275.0
42	192780.0
43	222285.0
44	210512.5
45	198740.0
46	174441.5
47	150143.0
48	127090.0
49	104037.0
50	88555.5
51	73074.0
52	56288.5
53	39503.0
54	30212.0
55	20921.0
56	17890.0
57	14859.0
58	13765.0
59	12671.0
60	11817.5
61	10964.0
62	9511.0
63	8058.0
64	7006.0
65	5954.0
66	4869.0
67	3784.0
68	3239.0
69	2694.0
70	2400.0
71	2106.0
72	1856.0
73	1606.0
74	1279.5
75	710.0
76	467.0
77	349.0
78	231.0
79	158.0
80	85.0
81	74.5
82	64.0
83	41.0
84	18.0
85	18.0
86	18.0
87	12.5
88	7.0
89	6.5
90	6.0
91	5.5
92	5.0
93	2.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1545429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.53692542781175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87234215824434	15.604599924902843
2	7.5661780709034705	2.9563971348957128
3	2.828342414467764	1.6577134450732118
4	1.5821614305781366	1.2364227953586004
5	1.0840672376632479	1.0589670390480375
6	0.7483099679638908	0.877180562459924
7	0.5821210374247888	0.7960998708689958
8	0.4651974374995217	0.7270822116509817
9	0.39247593063865305	0.6900995690188543
>10	3.1822447934385467	12.732024318848294
>50	0.569069614994255	7.993423779250889
>100	1.0915486695046013	48.12880394934018
>500	0.03194776593671974	4.0699856243883605
>1k	0.00366068151358247	1.0438444575720878
>5k	3.327892285074972E-4	0.4273553173230354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6573	0.4253187949753758	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2573	0.16649098729220171	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1876	0.12139024180340863	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1598	0.10340170916942804	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1569	0.10152520756372503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032677010720000725	0.0
2	0.0	0.0	0.0	0.12656679795707212	0.0
3	0.0	0.0	0.0	0.17917354986867723	0.0
4	0.0	0.0	0.0	0.2524218194430155	0.0
5	0.0	0.0	0.0	0.4262246923022669	0.0
6	0.0	0.0	0.0	0.5901274015176369	0.0
7	0.0	0.0	0.0	0.7037528090905503	0.0
8	0.0	0.0	0.0	0.9970694221475073	0.0
9	0.0	0.0	0.0	1.1081712585955097	0.0
10	0.0	0.0	0.0	1.3132275892325043	0.0
11	0.0	0.0	0.0	1.503530734831558	0.0
12	0.0	0.0	0.0	1.6730629488640372	0.0
13	0.0	0.0	0.0	1.7419111457077614	0.0
14	0.0	0.0	0.0	1.7666292013415046	0.0
15	0.0	0.0	0.0	1.8117946537822185	0.0
16	0.0	0.0	0.0	1.9199199704418644	0.0
17	0.0	0.0	0.0	2.050433892466105	0.0
18	0.0	0.0	0.0	2.2545843257762086	0.0
19	0.0	0.0	0.0	2.3371503964271407	0.0
20	0.0	0.0	0.0	2.4241812467606083	0.0
21	0.0	0.0	0.0	2.545506781612096	0.0
22	0.0	0.0	0.0	2.6643734522905937	0.0
23	0.0	0.0	0.0	2.8102229219200625	0.0
24	0.0	0.0	0.0	2.9226836043584012	0.0
25	0.0	0.0	0.0	3.0150851317012948	0.0
26	0.0	0.0	0.0	3.1134396986209008	0.0
27	0.0	0.0	0.0	3.2089471596559918	0.0
28	0.0	0.0	0.0	3.311701799306212	0.0
29	0.0	0.0	0.0	3.429597865705898	0.0
30	0.0	0.0	0.0	3.5886475535272084	0.0
31	0.0	0.0	0.0	3.736114696954697	0.0
32	0.0	0.0	0.0	3.867987464969274	0.0
33	0.0	0.0	0.0	4.00303087362797	0.0
34	0.0	0.0	0.0	4.1336095026041315	0.0
35	0.0	0.0	0.0	4.325659735905046	0.0
36	0.0	0.0	0.0	4.475262208745921	0.0
37	0.0	0.0	0.0	4.627711787471311	0.0
38	0.0	0.0	0.0	4.7727200667258085	0.0
39	0.0	0.0	0.0	4.918051880739911	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	20	7.0340413E-4	45.000004	25
AACCGTC	35	1.2122837E-7	45.000004	29
GGTACGA	20	7.0340413E-4	45.000004	8
ACGCCTA	40	6.8175723E-9	45.000004	7
CAAGTCG	35	1.2122837E-7	45.000004	1
ATTCGTC	40	6.8175723E-9	45.000004	38
AAGCGTG	35	1.2122837E-7	45.000004	1
TAACGTG	20	7.0340413E-4	45.000004	1
TCGATTG	30	2.165989E-6	45.000004	1
CCATGCG	20	7.0340413E-4	45.000004	1
CTACGCT	20	7.0340413E-4	45.000004	37
ACGCTAG	40	6.8175723E-9	45.000004	1
CCGACAG	20	7.0340413E-4	45.000004	11
CAGTCGC	20	7.0340413E-4	45.000004	37
TAGGCCG	20	7.0340413E-4	45.000004	1
ATACGTT	35	1.2122837E-7	45.000004	37
TAAATCG	20	7.0340413E-4	45.000004	17
GTGCGTA	20	7.0340413E-4	45.000004	16
CGTCAAT	20	7.0340413E-4	45.000004	18
ATACCGG	35	1.2122837E-7	45.000004	2
>>END_MODULE