##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545134_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1412592
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.872908808771392	31.0	31.0	33.0	30.0	33.0
2	31.19485031771382	31.0	31.0	33.0	30.0	34.0
3	31.352405365455844	31.0	31.0	34.0	28.0	34.0
4	35.20696351104919	37.0	35.0	37.0	33.0	37.0
5	35.05036486119134	37.0	35.0	37.0	32.0	37.0
6	35.14444510516837	37.0	35.0	37.0	32.0	37.0
7	35.54708861440529	36.0	35.0	37.0	35.0	37.0
8	35.638029947783934	37.0	35.0	37.0	35.0	37.0
9	37.510906192304645	39.0	37.0	39.0	35.0	39.0
10	37.01758681912399	39.0	37.0	39.0	33.0	39.0
11	36.52584752001994	39.0	35.0	39.0	32.0	39.0
12	34.75654329063169	35.0	34.0	39.0	30.0	39.0
13	34.172473014147045	35.0	33.0	39.0	26.0	39.0
14	34.685830020274786	35.0	33.0	40.0	25.0	41.0
15	35.29751265758266	35.0	33.0	40.0	30.0	41.0
16	35.60661464881579	35.0	34.0	40.0	31.0	41.0
17	35.485627130834665	35.0	34.0	39.0	31.0	41.0
18	35.59969403762728	36.0	35.0	39.0	31.0	41.0
19	35.43837073974651	36.0	34.0	39.0	31.0	41.0
20	35.111526895239386	35.0	34.0	39.0	30.0	41.0
21	34.93579887186109	35.0	34.0	39.0	29.0	41.0
22	34.75365639901684	35.0	34.0	39.0	29.0	41.0
23	34.75689441820427	35.0	34.0	39.0	29.0	41.0
24	34.908835672296036	35.0	34.0	39.0	30.0	41.0
25	34.81774709187083	35.0	34.0	38.0	30.0	41.0
26	34.588038867556946	35.0	34.0	38.0	29.0	41.0
27	34.575500923125716	35.0	34.0	38.0	29.0	41.0
28	34.709432730753115	35.0	34.0	38.0	29.0	41.0
29	34.72760075095994	35.0	34.0	38.0	29.0	41.0
30	34.711675416539244	35.0	34.0	38.0	29.0	41.0
31	34.39208490491239	35.0	34.0	38.0	29.0	41.0
32	34.21283923454189	35.0	34.0	38.0	29.0	41.0
33	33.94753686839512	35.0	33.0	38.0	27.0	41.0
34	33.76215425260797	35.0	33.0	38.0	25.0	41.0
35	33.51906212126361	35.0	33.0	38.0	24.0	41.0
36	33.21424445275069	35.0	33.0	38.0	23.0	41.0
37	33.245590375706506	35.0	32.0	38.0	23.0	41.0
38	33.348405625969846	35.0	33.0	38.0	23.0	41.0
39	33.39460297099233	35.0	33.0	38.0	23.0	41.0
40	32.971763962984355	35.0	32.0	38.0	23.0	41.0
41	33.14238152276099	35.0	32.0	38.0	23.0	41.0
42	33.14591686771552	35.0	32.0	38.0	23.0	41.0
43	33.11084800140451	35.0	32.0	38.0	23.0	41.0
44	33.122518745681695	35.0	33.0	38.0	23.0	41.0
45	33.21381686998086	35.0	33.0	38.0	23.0	40.0
46	33.256030049724195	35.0	33.0	38.0	23.0	40.0
47	33.1973336957876	35.0	33.0	38.0	23.0	40.0
48	33.25087994268692	35.0	33.0	38.0	23.0	40.0
49	33.33760845311314	35.0	33.0	38.0	24.0	40.0
50	33.01601028463987	35.0	32.0	38.0	24.0	40.0
51	32.698550607677234	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	5.0
14	12.0
15	42.0
16	124.0
17	380.0
18	853.0
19	1823.0
20	3205.0
21	5207.0
22	7827.0
23	11270.0
24	16066.0
25	22272.0
26	28310.0
27	32624.0
28	35681.0
29	40993.0
30	49071.0
31	60361.0
32	78096.0
33	104688.0
34	202015.0
35	299477.0
36	65845.0
37	77461.0
38	109130.0
39	159697.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.49472175971547	25.085728929513973	30.968036064199712	13.451513246570842
2	28.66907075786922	26.447834902080714	32.95374743733505	11.92934690271501
3	26.153128433404692	26.750611641578036	33.08202226828412	14.014237656733156
4	25.45950989386886	29.95436757393501	31.30047458855777	13.285647943638363
5	21.11352747290088	33.02036256753542	33.41339891490253	12.452711044661163
6	19.789365931564102	41.97093003499949	28.39029245528787	9.849411578148539
7	87.97649993770317	4.63707850532921	6.344294743280438	1.0421268136871793
8	91.47375887729791	2.009497434503381	5.614784736144619	0.9019589520540963
9	88.67146352237589	2.9707799562789536	7.207035010816995	1.1507215105281638
10	68.7794494093128	15.809660538924192	10.647943638361253	4.762946413401747
11	65.67048376318144	13.154399855018292	15.469222535594144	5.7058938462061235
12	57.76225548495248	19.841610316354615	15.843782210291435	6.552351988401463
13	17.470366531878987	59.5251849083104	16.54653289838821	6.457915661422406
14	7.567011564556503	59.69529772220146	24.602149806879837	8.135540906362206
15	5.91196891954648	21.70973642778665	65.04418827233907	7.334106380327794
16	6.859093071460125	13.03957547543806	64.00269858529568	16.09863286780613
17	7.5946203857872625	12.657299489166016	31.537485700046442	48.21059442500028
18	14.772701530236615	19.337430765571376	48.53921018949563	17.350657514696387
19	35.63498872993759	15.868700941248429	29.705958974707485	18.790351354106495
20	35.09916522251294	18.982763600530088	28.77129418827234	17.146776988684632
21	15.660431320579473	34.14078516655906	32.306993102042206	17.89179041081926
22	18.647422610350333	23.384529998754065	20.18863196167046	37.77941542922514
23	10.581824051106052	34.86045510664084	24.66593326310782	29.89178757914529
24	8.079261386161043	18.478725633445467	61.21378288989319	12.2282300905003
25	7.203566210200822	22.30969735068583	54.64401610656155	15.84272033255179
26	7.227210687870242	44.33035158064041	30.284116008019303	18.158321723470046
27	6.972855573300713	56.73924247057891	24.871583585352315	11.416318370768064
28	6.45133201943661	36.757889043687065	46.18955791905943	10.601221017816892
29	7.191248428420945	25.030865246299	44.17843227202193	23.599454053258125
30	12.917459535378933	44.81881534087691	29.45917858801409	12.804546535730063
31	34.704925413707564	30.4684579836216	25.540637353177704	9.28597924949313
32	40.27100535752716	19.630013478768106	31.496709594844084	8.602271568860647
33	40.3469650118364	25.27007090511627	19.11932107784838	15.263643005198954
34	20.062339302502068	25.350915197027874	23.381061198137893	31.205684302332166
35	17.529194558655295	23.890054594674186	36.49900325076172	22.081747595908794
36	43.16894050086649	22.797453192429238	22.536302060325983	11.497304246378288
37	15.92880322131231	46.70216169991052	27.303849943932857	10.065185134844315
38	15.564579156614226	45.52708779321984	21.15026844269258	17.758064607473354
39	19.755739803142024	42.27094589237373	25.225472040051194	12.747842264433043
40	29.649254703410467	21.980656835094635	24.132304303011768	24.237784158483127
41	12.103707227564648	21.458354570888126	27.863601096424162	38.574337105123064
42	15.574136056271026	17.38385889202261	26.78310510041116	40.258899951295206
43	15.778299749680022	18.013198432385288	31.242920815069038	34.965581002865655
44	9.934644964717343	30.0108594696841	37.340435171656075	22.714060393942486
45	8.945045703217914	57.609699049690214	20.097806019006196	13.347449228085676
46	18.324824153046315	45.12569800763419	22.196784351036957	14.352693488282533
47	17.068056452252314	29.87876187887231	23.91688470556254	29.13629696331283
48	17.90524086218809	26.796980302875852	41.61923612762921	13.678542707306852
49	24.162461630817674	24.37632380758209	40.57852515092822	10.882689410672013
50	19.37735736858201	47.5937142501161	21.804880673258804	11.224047708043086
51	15.804138774678039	41.7792964989183	22.978821910360526	19.437742816043134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2351.0
1	2409.0
2	2467.0
3	5535.5
4	8604.0
5	6931.0
6	5258.0
7	5931.0
8	6604.0
9	7218.5
10	7833.0
11	8066.0
12	8299.0
13	8012.5
14	7726.0
15	7250.0
16	6774.0
17	6254.5
18	5735.0
19	5377.5
20	5020.0
21	5088.5
22	5157.0
23	5250.0
24	5343.0
25	6244.5
26	12097.0
27	17048.0
28	20918.5
29	24789.0
30	24277.5
31	23766.0
32	29836.5
33	35907.0
34	38904.0
35	41901.0
36	47112.0
37	52323.0
38	66848.5
39	81374.0
40	121650.0
41	161926.0
42	210784.0
43	259642.0
44	244541.5
45	229441.0
46	195754.5
47	162068.0
48	129863.0
49	97658.0
50	84091.5
51	70525.0
52	51014.0
53	31503.0
54	22006.5
55	12510.0
56	9795.0
57	7080.0
58	6214.5
59	5349.0
60	4764.0
61	4179.0
62	3674.5
63	3170.0
64	2635.5
65	2101.0
66	1778.0
67	1455.0
68	1155.0
69	855.0
70	758.0
71	661.0
72	547.0
73	433.0
74	384.0
75	249.5
76	164.0
77	106.5
78	49.0
79	39.5
80	30.0
81	19.0
82	8.0
83	11.0
84	14.0
85	10.5
86	7.0
87	4.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1412592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.128490554343083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18085830045578	12.281438479919096
2	7.499693729336669	2.2691809148947173
3	2.5712334377244717	1.166966423268773
4	1.4090310665601342	0.852660527249238
5	0.8682581855436186	0.6567717879363849
6	0.6203252804732354	0.5630751087755739
7	0.4569517507394254	0.4839093171396352
8	0.3570565465336705	0.4321381273280797
9	0.2907518942555465	0.39587735573121546
>10	2.834662866109343	9.078219868161272
>50	0.4621769749798564	5.002008778346362
>100	1.332516339447875	54.63931079467923
>500	0.10755947893322643	10.19779086035629
>1k	0.00892414890712359	1.980651656214133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4572	0.3236603350436644	No Hit
ATTGAAGGGGAATAATTTAGATTTTGATGAGATTCAGGAAATTATTGAAAG	2065	0.14618516882440225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2009	0.1422208252630625	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1990	0.1408757801261794	No Hit
AAGAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1602	0.1134085425940399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02640535979249493	0.0
2	0.0	0.0	0.0	0.09415315958181839	0.0
3	0.0	0.0	0.0	0.1307525456749012	0.0
4	0.0	0.0	0.0	0.19772163512181862	0.0
5	0.0	0.0	0.0	0.33201377326220166	0.0
6	0.0	0.0	0.0	0.45420050517063665	0.0
7	0.0	0.0	0.0	0.5337705437946697	0.0
8	0.0	0.0	0.0	0.7249085369306919	0.0
9	0.0	0.0	0.0	0.79761176617169	0.0
10	0.0	0.0	0.0	0.9244707601345612	0.0
11	0.0	0.0	0.0	1.063293576630761	0.0
12	0.0	0.0	0.0	1.1711095631293396	0.0
13	0.0	0.0	0.0	1.217549016276462	0.0
14	0.0	0.0	0.0	1.234963811206633	0.0
15	0.0	0.0	0.0	1.2642008449715134	0.0
16	0.0	0.0	0.0	1.335629820924938	0.0
17	0.0	0.0	0.0	1.417535990576189	0.0
18	0.0	0.0	0.0	1.559473648442013	0.0
19	0.0	0.0	0.0	1.6131338702187186	0.0
20	0.0	0.0	0.0	1.667006467543353	0.0
21	0.0	0.0	0.0	1.7469304654139342	0.0
22	0.0	0.0	0.0	1.822394576778008	0.0
23	0.0	0.0	0.0	1.9185299081404963	0.0
24	0.0	0.0	0.0	1.9888970063542764	0.0
25	0.0	0.0	0.0	2.043831481418555	0.0
26	0.0	0.0	0.0	2.1091723583313513	0.0
27	0.0	0.0	0.0	2.16800038510766	0.0
28	0.0	0.0	0.0	2.231005130993238	0.0
29	0.0	0.0	0.0	2.2967707590018915	0.0
30	0.0	0.0	0.0	2.403312492212897	0.0
31	0.0	0.0	0.0	2.5060314655611813	0.0
32	0.0	0.0	0.0	2.5830529976100673	0.0
33	0.0	0.0	0.0	2.6663042123982015	0.0
34	0.0	0.0	0.0	2.7538029381449136	0.0
35	0.0	0.0	0.0	2.8972980166955495	0.0
36	0.0	0.0	0.0	3.006388256481702	0.0
37	0.0	0.0	0.0	3.11618641476095	0.0
38	0.0	0.0	0.0	3.201773760576302	0.0
39	0.0	0.0	0.0	3.3061917383080184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	30	2.1658561E-6	45.000004	20
CTTAACG	30	2.1658561E-6	45.000004	1
CTCACGT	60	0.0	45.000004	45
CGACGGT	60	0.0	45.000004	28
ACGTTAG	60	0.0	45.000004	1
TTCACGA	30	2.1658561E-6	45.000004	19
GTTAGCG	30	2.1658561E-6	45.000004	14
CGATTGC	60	0.0	45.000004	10
GTAGCGT	30	2.1658561E-6	45.000004	9
CTAACGG	60	0.0	45.000004	2
ACGATAC	30	2.1658561E-6	45.000004	22
TATCTAG	60	0.0	45.000004	1
CTACGCG	30	2.1658561E-6	45.000004	1
CGTAGAA	30	2.1658561E-6	45.000004	45
TACGAAG	125	0.0	45.000004	1
ACGTGCG	30	2.1658561E-6	45.000004	1
ACCAGAC	30	2.1658561E-6	45.000004	22
CGAGCAA	30	2.1658561E-6	45.000004	19
ACGTCAT	30	2.1658561E-6	45.000004	10
AATTACG	60	0.0	45.000004	40
>>END_MODULE