##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545133_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1203780 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.853044576251474 31.0 31.0 33.0 30.0 33.0 2 31.170074266061906 31.0 31.0 33.0 30.0 34.0 3 31.329129076741598 31.0 31.0 34.0 30.0 34.0 4 35.19613218362159 35.0 35.0 37.0 33.0 37.0 5 35.07841466048613 37.0 35.0 37.0 32.0 37.0 6 35.17270514545847 37.0 35.0 37.0 32.0 37.0 7 35.51224974663145 36.0 35.0 37.0 35.0 37.0 8 35.57387230224792 37.0 35.0 37.0 35.0 37.0 9 37.43694196613999 39.0 37.0 39.0 35.0 39.0 10 36.91812872784064 39.0 37.0 39.0 32.0 39.0 11 36.48075478908106 38.0 35.0 39.0 32.0 39.0 12 35.194253102726414 35.0 35.0 39.0 31.0 39.0 13 34.73947897456346 35.0 33.0 39.0 30.0 39.0 14 35.43722274834272 36.0 33.0 40.0 29.0 41.0 15 35.89597268603898 36.0 34.0 40.0 31.0 41.0 16 36.115498679160645 36.0 34.0 40.0 32.0 41.0 17 35.97581700975261 36.0 34.0 40.0 31.0 41.0 18 36.06207446543388 36.0 35.0 40.0 31.0 41.0 19 35.91485404309758 36.0 35.0 40.0 31.0 41.0 20 35.64207662529698 36.0 34.0 40.0 31.0 41.0 21 35.50581750818256 35.0 34.0 40.0 30.0 41.0 22 35.362013823123824 35.0 34.0 40.0 30.0 41.0 23 35.299427636279056 35.0 34.0 40.0 30.0 41.0 24 35.39602169831697 35.0 34.0 40.0 30.0 41.0 25 35.31351575869345 35.0 34.0 40.0 30.0 41.0 26 35.09291731047201 35.0 34.0 39.0 29.0 41.0 27 35.08446809217631 35.0 34.0 39.0 29.0 41.0 28 35.18446809217631 36.0 34.0 40.0 29.0 41.0 29 35.17687783482032 36.0 34.0 40.0 29.0 41.0 30 35.138319294223194 36.0 34.0 39.0 29.0 41.0 31 34.907690774061706 35.0 34.0 40.0 29.0 41.0 32 34.75380966621808 35.0 34.0 40.0 29.0 41.0 33 34.49146937147984 35.0 33.0 40.0 27.0 41.0 34 34.29064862350263 35.0 34.0 40.0 26.0 41.0 35 34.045968532456094 35.0 33.0 40.0 25.0 41.0 36 33.782660452906676 35.0 33.0 39.0 23.0 41.0 37 33.830944192460414 35.0 33.0 39.0 23.0 41.0 38 33.904827294023825 35.0 33.0 39.0 24.0 41.0 39 33.913227500041536 35.0 33.0 40.0 24.0 41.0 40 33.54183571748991 35.0 33.0 39.0 23.0 41.0 41 33.64690142717108 35.0 33.0 39.0 23.0 41.0 42 33.66543471398428 35.0 33.0 39.0 23.0 41.0 43 33.6203525561149 35.0 33.0 39.0 23.0 41.0 44 33.59307514662147 35.0 33.0 39.0 23.0 41.0 45 33.66984249613717 35.0 33.0 39.0 23.0 41.0 46 33.674553489840335 35.0 33.0 39.0 23.0 41.0 47 33.59727774178006 35.0 33.0 39.0 23.0 41.0 48 33.65956071707455 35.0 33.0 39.0 23.0 41.0 49 33.70875326056256 35.0 33.0 39.0 24.0 41.0 50 33.47724335011381 35.0 33.0 39.0 24.0 41.0 51 33.19724866669989 35.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 3.0 12 1.0 13 1.0 14 3.0 15 21.0 16 95.0 17 253.0 18 641.0 19 1328.0 20 2324.0 21 3998.0 22 5914.0 23 8895.0 24 12870.0 25 17628.0 26 22651.0 27 26261.0 28 28039.0 29 32504.0 30 38751.0 31 48050.0 32 61182.0 33 82718.0 34 147964.0 35 217100.0 36 69380.0 37 84350.0 38 121804.0 39 168985.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.308195849739988 25.499426805562482 30.906560999518184 13.285816345179352 2 29.994434198940006 26.245742577547393 31.59198524647361 12.167837977038994 3 26.34816993138281 26.538902457259635 34.17310472013158 12.939822891225973 4 23.508282244263903 27.678313313063853 34.91684527073053 13.896559171941716 5 21.066723155393845 32.88632474372394 33.620927411985576 12.426024688896643 6 18.93385834620944 42.986259947831 29.15574274369071 8.924138962268852 7 87.66826164249282 4.387761883400621 6.750984399142701 1.1929920749638638 8 90.18774194620279 2.515409792486999 6.264184485537225 1.0326637757729817 9 86.70247055109738 3.820880891857316 7.837893967336224 1.6387545897090832 10 57.86215089135889 22.95103756500357 12.76387712040406 6.422934423233481 11 52.1644320390769 16.262273837412152 22.237119739487284 9.336174384023659 12 50.449168452707305 19.753526391865623 20.861951519380618 8.935353636046454 13 16.931416039475653 52.392879097509514 19.69604080479822 10.979664058216617 14 9.248866071873598 51.91372177640433 25.169798468158632 13.667613683563443 15 7.783149744970011 22.91066473940421 56.195567296349836 13.110618219275946 16 8.334413264882286 17.27699413514097 55.18666201465384 19.2019305853229 17 8.688298526308794 17.777750087225243 32.177308146006744 41.35664324045922 18 14.932296599046339 21.864127996810048 45.65709681170978 17.546478592433836 19 30.702121650135407 20.25419927229228 28.79953147585107 20.244147601721245 20 31.401585007227233 20.319493595175196 29.91958663543172 18.359334762165844 21 14.157819535131003 35.26873681237435 31.21924271876921 19.354200933725433 22 17.38797786971041 25.61946535081161 20.821744837096478 36.1708119423815 23 11.397597567661865 36.335459967768195 25.681436807390057 26.58550565717988 24 10.292412234793732 19.45571449932712 57.278489424977984 12.973383840901162 25 9.072089584475568 25.34973167854591 47.899117778996164 17.679060957982358 26 9.276778148831182 40.88853444981641 30.696638920733026 19.138048480619382 27 7.743026134343485 49.7968067254814 26.475186495871338 15.984980644303775 28 7.733805180348568 33.41424512784728 42.9540281446776 15.89792154712655 29 7.7873863995082155 22.718104637059927 44.29970592633205 25.1948030370998 30 12.480021266344348 43.28805927993487 30.330542125637578 13.901377328083205 31 30.58798119257672 31.286946119722874 26.109920417352008 12.015152270348404 32 34.6552526209108 21.285700044858697 29.46792603299606 14.591121301234445 33 35.17195833125654 27.108275598531296 20.631676884480555 17.08808918573161 34 18.36315605841599 24.810015119041683 25.04892920633338 31.777899616208945 35 17.619913937762714 23.844639385934308 38.12939241389623 20.40605426240675 36 37.172739204838095 25.61182275831132 23.42413065510309 13.791307381747494 37 14.155161242087425 45.46420442273505 28.674093272857164 11.706541062320358 38 14.932711957334396 42.06017710877403 26.433401452092575 16.573709481799 39 18.123411254548174 41.32549136885477 27.08883683065012 13.462260545946933 40 29.025320241240095 24.007459834853545 25.53855355629766 21.4286663676087 41 12.666101779394905 22.704314741896358 30.240990878731992 34.38859259997674 42 14.328947149811427 19.48927544900231 28.10272641180282 38.07905098938344 43 14.872318862250577 21.43099237402183 29.03337819202844 34.66331057169915 44 10.606755387196996 29.319809267474124 36.22771602784562 23.845719317483262 45 9.98122580537972 51.52660785193308 21.37774344149263 17.11442290119457 46 15.945438535280532 43.76115237003439 24.120852647493727 16.172556447191347 47 15.17361976440878 28.843725597700576 27.73878947980528 28.243865158085363 48 16.216169067437573 25.203940919437105 41.75173204406121 16.828157969064115 49 20.509644619448736 25.826978351526027 39.95198458190035 13.71139244712489 50 16.761119141371346 43.03660137234379 26.95957733140607 13.242702154878799 51 14.184319393909187 38.76248151655618 25.689328614863182 21.36387047467145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1544.0 1 1985.5 2 2427.0 3 5596.0 4 8765.0 5 6815.0 6 4865.0 7 5078.0 8 5291.0 9 5720.0 10 6149.0 11 6305.0 12 6461.0 13 6444.0 14 6427.0 15 6190.0 16 5953.0 17 5835.0 18 5717.0 19 5234.0 20 4751.0 21 4247.5 22 3744.0 23 4625.0 24 5506.0 25 6667.0 26 11157.5 27 14487.0 28 15828.5 29 17170.0 30 24477.0 31 31784.0 32 37161.5 33 42539.0 34 50173.5 35 57808.0 36 60146.5 37 62485.0 38 74851.0 39 87217.0 40 115195.0 41 143173.0 42 161647.0 43 180121.0 44 170262.5 45 160404.0 46 135408.0 47 110412.0 48 94324.0 49 78236.0 50 65278.5 51 52321.0 52 42337.5 53 32354.0 54 26448.0 55 20542.0 56 16668.5 57 12795.0 58 10394.0 59 7993.0 60 6411.0 61 4829.0 62 4239.5 63 3650.0 64 3149.0 65 2648.0 66 2280.0 67 1912.0 68 1578.0 69 1244.0 70 1101.0 71 958.0 72 768.0 73 578.0 74 479.5 75 274.5 76 168.0 77 121.0 78 74.0 79 54.0 80 34.0 81 24.5 82 15.0 83 16.0 84 17.0 85 10.0 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1203780.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.44361959836198 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56775740791912 14.053933121168516 2 7.093925804379506 2.474874863812002 3 2.5376558492881482 1.3279770991962199 4 1.4050570045370394 0.9803711960463233 5 1.0029388474997807 0.8747441868102876 6 0.6847600035181587 0.7166815810526262 7 0.5647255535659286 0.6895600413714915 8 0.48645125310682846 0.6788376489873613 9 0.39113119129894053 0.6140469342665581 >10 3.193687083742945 11.359723470052826 >50 0.6102855866713405 7.69502596422214 >100 1.4147529509192178 52.8126366190565 >500 0.04256694139006436 4.502350230772395 >1k 0.004304522163040217 1.219237043184742 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4743 0.3940088720530329 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2068 0.17179218794131818 No Hit TGTTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA 1342 0.11148216451511074 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1225 0.10176278057452358 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.024506138995497515 0.0 2 0.0 0.0 0.0 0.08257322766618486 0.0 3 0.0 0.0 0.0 0.11397431424346642 0.0 4 0.0 0.0 0.0 0.17096147136519962 0.0 5 0.0 0.0 0.0 0.28825865191314026 0.0 6 0.0 0.0 0.0 0.4055558324610809 0.0 7 0.0 0.0 0.0 0.4961870109156158 0.0 8 0.0 0.0 0.0 0.7531276479090864 0.0 9 0.0 0.0 0.0 0.8577979365000249 0.0 10 0.0 0.0 0.0 1.0237751084085132 0.0 11 0.0 0.0 0.0 1.1684028643107545 0.0 12 0.0 0.0 0.0 1.2988253667613683 0.0 13 0.0 0.0 0.0 1.3426041303228164 0.0 14 0.0 0.0 0.0 1.363122829752945 0.0 15 0.0 0.0 0.0 1.3872136104603832 0.0 16 0.0 0.0 0.0 1.4560800146206117 0.0 17 0.0 0.0 0.0 1.5428068251673894 0.0 18 0.0 0.0 0.0 1.687766867700078 0.0 19 0.0 0.0 0.0 1.74201266012062 0.0 20 0.0 0.0 0.0 1.8051471199056306 0.0 21 0.0 0.0 0.0 1.888551064147934 0.0 22 0.0 0.0 0.0 1.9804283174666468 0.0 23 0.0 0.0 0.0 2.0942364883948894 0.0 24 0.0 0.0 0.0 2.1765605010882387 0.0 25 0.0 0.0 0.0 2.244845403645184 0.0 26 0.0 0.0 0.0 2.3196929671534665 0.0 27 0.0 0.0 0.0 2.3974480386781636 0.0 28 0.0 0.0 0.0 2.4732093904201764 0.0 29 0.0 0.0 0.0 2.5540381132765124 0.0 30 0.0 0.0 0.0 2.6820515376563825 0.0 31 0.0 0.0 0.0 2.7869710412201565 0.0 32 0.0 0.0 0.0 2.8803435843758827 0.0 33 0.0 0.0 0.0 2.9842662280483143 0.0 34 0.0 0.0 0.0 3.111033577564007 0.0 35 0.0 0.0 0.0 3.2835734104238314 0.0 36 0.0 0.0 0.0 3.410091544966688 0.0 37 0.0 0.0 0.0 3.5231520709764244 0.0 38 0.0 0.0 0.0 3.645101264350629 0.0 39 0.0 0.0 0.0 3.783498645931981 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTAG 20 7.033419E-4 45.000004 6 ACCGGGT 30 2.165587E-6 45.000004 4 CGAACGG 30 2.165587E-6 45.000004 2 ATCTACG 20 7.033419E-4 45.000004 1 ACGTTTA 40 6.8157533E-9 45.000004 26 ACGCATA 20 7.033419E-4 45.000004 34 GACAATG 30 2.165587E-6 45.000004 1 CGCATAG 20 7.033419E-4 45.000004 35 GGCAACG 40 6.8157533E-9 45.000004 8 TTCACGG 40 6.8157533E-9 45.000004 2 ACCGACT 20 7.033419E-4 45.000004 25 TGAACGT 20 7.033419E-4 45.000004 45 AAGCGTG 30 2.165587E-6 45.000004 3 TCTAGCG 30 2.165587E-6 45.000004 1 CGCCTAG 30 2.165587E-6 45.000004 39 TCTACCG 20 7.033419E-4 45.000004 1 TATCTCG 30 2.165587E-6 45.000004 1 CTCGGTA 40 6.8157533E-9 45.000004 13 CGTACAG 20 7.033419E-4 45.000004 15 ACGTATA 30 2.165587E-6 45.000004 13 >>END_MODULE