##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545132_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1643167
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.877089182049055	31.0	31.0	33.0	30.0	33.0
2	31.17651583801281	31.0	31.0	33.0	30.0	34.0
3	31.34378124682397	31.0	31.0	34.0	28.0	34.0
4	35.18029329946378	37.0	35.0	37.0	32.0	37.0
5	35.0659811206043	37.0	35.0	37.0	32.0	37.0
6	35.13272235871339	37.0	35.0	37.0	32.0	37.0
7	35.560676425463754	36.0	35.0	37.0	35.0	37.0
8	35.63478696930988	37.0	35.0	37.0	35.0	37.0
9	37.47733127551856	39.0	37.0	39.0	35.0	39.0
10	37.02003204786854	39.0	37.0	39.0	33.0	39.0
11	36.56174631063063	39.0	35.0	39.0	32.0	39.0
12	34.8417026388675	35.0	34.0	39.0	30.0	39.0
13	34.273418343966256	35.0	33.0	39.0	27.0	39.0
14	34.865773837960475	35.0	33.0	40.0	26.0	41.0
15	35.409976587893986	35.0	33.0	40.0	30.0	41.0
16	35.67800473110767	35.0	34.0	40.0	31.0	41.0
17	35.55296022863166	35.0	34.0	40.0	31.0	41.0
18	35.69340000133888	36.0	35.0	39.0	31.0	41.0
19	35.531101220995794	36.0	34.0	39.0	31.0	41.0
20	35.185930584048975	35.0	34.0	39.0	30.0	41.0
21	35.029057910729705	35.0	34.0	39.0	30.0	41.0
22	34.85194383772313	35.0	34.0	39.0	29.0	41.0
23	34.845796562370104	35.0	34.0	39.0	29.0	41.0
24	34.955060562925134	35.0	34.0	39.0	30.0	41.0
25	34.881980346489435	35.0	34.0	39.0	30.0	41.0
26	34.64792805600405	35.0	34.0	38.0	29.0	41.0
27	34.641609769426964	35.0	34.0	39.0	29.0	41.0
28	34.79262972053358	35.0	34.0	38.0	29.0	41.0
29	34.808956728074506	35.0	34.0	38.0	29.0	41.0
30	34.778745556598935	35.0	34.0	38.0	29.0	41.0
31	34.440297912506765	35.0	34.0	38.0	29.0	41.0
32	34.26037767311539	35.0	34.0	38.0	28.0	41.0
33	34.01503012171009	35.0	33.0	38.0	27.0	41.0
34	33.858176922978615	35.0	33.0	38.0	25.0	41.0
35	33.62571911436878	35.0	33.0	38.0	24.0	41.0
36	33.334494302770196	35.0	33.0	38.0	23.0	41.0
37	33.361189702568275	35.0	33.0	38.0	23.0	41.0
38	33.46123187722246	35.0	33.0	38.0	23.0	41.0
39	33.52308621095725	35.0	33.0	38.0	23.0	41.0
40	33.06344881561034	35.0	32.0	38.0	23.0	41.0
41	33.21988331070427	35.0	32.0	38.0	23.0	41.0
42	33.21582468489204	35.0	32.0	38.0	23.0	41.0
43	33.20778046297181	35.0	33.0	38.0	23.0	41.0
44	33.17656209015882	35.0	33.0	38.0	23.0	41.0
45	33.263444312111915	35.0	33.0	38.0	23.0	40.0
46	33.27005167460155	35.0	33.0	38.0	23.0	40.0
47	33.23578796312243	35.0	33.0	38.0	23.0	40.0
48	33.29793380709325	35.0	33.0	38.0	23.0	40.0
49	33.35161063969761	35.0	33.0	38.0	24.0	40.0
50	33.07286477880824	35.0	32.0	38.0	24.0	40.0
51	32.81360141726313	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	2.0
14	11.0
15	33.0
16	141.0
17	422.0
18	1049.0
19	2051.0
20	3779.0
21	6031.0
22	9027.0
23	13278.0
24	18848.0
25	25622.0
26	32727.0
27	37116.0
28	41398.0
29	47150.0
30	56689.0
31	69345.0
32	89033.0
33	120113.0
34	228357.0
35	338536.0
36	79297.0
37	94739.0
38	134682.0
39	193561.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.843770596658764	25.22920676961015	28.854279571096548	13.072743062634535
2	29.42512842577778	26.61019847647865	31.4948511015618	12.469821996181764
3	28.47136048861741	26.643731282334667	32.740555281356066	12.144352947691866
4	25.297611259232934	27.918099621036692	33.279271066178914	13.505018053551465
5	21.718486313320557	33.6530614356301	31.875640151001083	12.75281210004826
6	18.206548695293908	42.6400968373878	29.801109686355677	9.352244780962616
7	87.33804902362328	4.873150446667928	6.63194915671992	1.1568513729888685
8	90.15243125014074	2.6063084275670096	6.17502664062752	1.0662336816647364
9	85.92857573210757	4.693801664712108	7.562712737049855	1.8149098661304663
10	66.11312179468064	16.775044776337403	11.545935379666219	5.565898049315742
11	63.745072777143164	13.614440893713178	16.310454141301523	6.330032187842137
12	57.41479715695361	19.80285631344836	16.719907349648576	6.062439179949451
13	16.90558537263711	58.74801526564251	16.077428526741347	8.268970834979038
14	8.491772290947907	58.66689143586744	23.613728854096998	9.227607419087652
15	7.5029500957602	20.594498307232314	63.768929147189546	8.133622449817942
16	7.695505082563124	14.108304268525353	61.301194583386845	16.894996065524683
17	7.794886338394089	14.458238267930163	30.4030570234188	47.343818370256955
18	15.41005874631124	21.74155152823785	46.694584299709035	16.153805425741872
19	37.32426466695108	16.507877775052687	27.56220152912029	18.605656028875945
20	36.00668708658341	18.972386860252183	28.0921537494363	16.928772303728106
21	15.109115506823104	36.690245118116415	31.13018944513857	17.070449929921914
22	19.14595412395697	24.980601484815605	18.840811676475976	37.03263271475145
23	11.224543822995471	36.283530523677754	23.834643709373424	28.657281943953354
24	8.872865630821456	18.88785497761335	61.305332933292846	10.933946458272349
25	7.889277231103107	24.093108004238157	52.48389238586218	15.533722378796554
26	7.960298618460571	44.34521871483544	29.26348934709619	18.430993319607804
27	8.066556838105926	55.005547214616655	25.797073578035583	11.130822369241836
28	6.9153044091075335	35.83750160513204	46.56106165715354	10.686132328606892
29	7.268889893723523	25.18831013524493	43.42784391361316	24.11495605741839
30	13.221967091598117	46.4168279913119	28.320189000874528	12.041015916215454
31	36.166317848398855	30.07935285944764	24.01977400958028	9.734555282573226
32	39.591593550746815	20.39019770966676	29.90615074426397	10.112057995322447
33	39.223280409112405	26.828557292107252	18.8837774857942	15.064384812986143
34	19.30388085934053	24.766015870571888	24.377193553668008	31.552909716419574
35	18.26290328371979	23.064362904074876	37.661479326203605	21.011254486001725
36	42.86734093369694	25.141023401760137	21.193950462734463	10.79768520180846
37	14.335852655268758	48.74099832822835	27.4350081275975	9.488140888905388
38	14.841035634235594	46.84788582049177	20.895745837154713	17.41533270811792
39	20.264525760315294	41.97242276652343	25.723191860596035	12.039859612565246
40	30.65762640072494	22.092885263640273	23.34552726533578	23.903961070299
41	11.65961828590764	22.392124476696527	27.451135520613544	38.49712171678228
42	14.978635768610252	18.669374445811044	25.46131951286753	40.89067027271117
43	16.809916460104176	18.943053262389036	29.805613184782803	34.441417092723995
44	10.287511859719675	30.703695972472673	37.47196724374333	21.53682492406432
45	9.67400148615448	58.47111097046132	18.988757685615642	12.866129857768566
46	17.254423926478562	45.4564873807714	22.360843420054078	14.928245272695959
47	17.001071710909482	28.762444718035358	24.617278706303132	29.61920486475203
48	17.50996703317435	26.095217345528482	42.331424620869335	14.063391000427833
49	22.75672527503291	25.687103015092198	40.59867317199043	10.957498537884463
50	18.324674241875595	48.34128241377778	21.6394316584985	11.694611685848121
51	15.735892943322256	41.59863239707224	23.038924223770316	19.62655043583519
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1978.0
1	2447.5
2	2917.0
3	6629.5
4	10342.0
5	8614.0
6	6886.0
7	7358.0
8	7830.0
9	8441.5
10	9053.0
11	9152.0
12	9251.0
13	8848.5
14	8446.0
15	7807.5
16	7169.0
17	6529.0
18	5889.0
19	6437.5
20	6986.0
21	6395.5
22	5805.0
23	6663.5
24	7522.0
25	8118.0
26	10454.0
27	12194.0
28	19410.5
29	26627.0
30	32092.0
31	37557.0
32	44657.5
33	51758.0
34	55930.5
35	60103.0
36	66691.5
37	73280.0
38	82721.0
39	92162.0
40	144639.5
41	197117.0
42	232915.5
43	268714.0
44	268790.5
45	268867.0
46	220976.0
47	173085.0
48	139951.5
49	106818.0
50	91045.5
51	75273.0
52	57427.0
53	39581.0
54	28775.0
55	17969.0
56	14866.0
57	11763.0
58	10169.5
59	8576.0
60	7828.0
61	7080.0
62	6167.0
63	5254.0
64	4343.0
65	3432.0
66	2945.5
67	2459.0
68	2094.0
69	1729.0
70	1453.5
71	1178.0
72	991.5
73	805.0
74	661.0
75	360.5
76	204.0
77	179.0
78	154.0
79	115.5
80	77.0
81	48.5
82	20.0
83	19.0
84	18.0
85	11.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1643167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.952999399721149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36167128104368	12.166010218257556
2	7.669980643499974	2.293784319162559
3	2.5853002717071627	1.159739802328084
4	1.36034217792817	0.8136478307990114
5	0.8381848727045097	0.6266688949202941
6	0.6016944534610417	0.53982820808511
7	0.4514965216142021	0.4725859051671345
8	0.3570822968308188	0.42715610961298267
9	0.2859319967172124	0.3847986877746192
>10	2.724212933823407	8.711670833345734
>50	0.43888424155401984	4.700579378496141
>100	1.0927238689200596	44.28583740352912
>500	0.21332384600417736	19.301590157532868
>1k	0.019170594191579994	4.1161022509887975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4838	0.29443142419486273	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2568	0.15628356703852986	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2259	0.13747841820094975	No Hit
GGTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2068	0.12585452361202484	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2046	0.1245156457012586	No Hit
GTAGATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1881	0.11447406137051194	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1818	0.11064000189877231	No Hit
GTATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1649	0.1003549852206136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01807485179534399	0.0
2	0.0	0.0	0.0	0.06797848301481225	0.0
3	0.0	0.0	0.0	0.09767722939908116	0.0
4	0.0	0.0	0.0	0.15190178478511313	0.0
5	0.0	0.0	0.0	0.26199406390220836	0.0
6	0.0	0.0	0.0	0.3837102376082285	0.0
7	0.0	0.0	0.0	0.46179116304064044	0.0
8	0.0	0.0	0.0	0.6981031142908786	0.0
9	0.0	0.0	0.0	0.8015010038541427	0.0
10	0.0	0.0	0.0	0.973120808779631	0.0
11	0.0	0.0	0.0	1.1032962565582196	0.0
12	0.0	0.0	0.0	1.215275136367758	0.0
13	0.0	0.0	0.0	1.263718173502754	0.0
14	0.0	0.0	0.0	1.2802107150399198	0.0
15	0.0	0.0	0.0	1.3070491313420973	0.0
16	0.0	0.0	0.0	1.3760622018334108	0.0
17	0.0	0.0	0.0	1.4523782427470853	0.0
18	0.0	0.0	0.0	1.6026368591871671	0.0
19	0.0	0.0	0.0	1.6513841867564283	0.0
20	0.0	0.0	0.0	1.7053653097950483	0.0
21	0.0	0.0	0.0	1.7801598985373976	0.0
22	0.0	0.0	0.0	1.8509987116343014	0.0
23	0.0	0.0	0.0	1.9389995052237539	0.0
24	0.0	0.0	0.0	2.0025353478982963	0.0
25	0.0	0.0	0.0	2.054690728331326	0.0
26	0.0	0.0	0.0	2.109463006499035	0.0
27	6.0858086853010074E-5	0.0	0.0	2.1659393110986285	0.0
28	6.0858086853010074E-5	0.0	0.0	2.223024196566752	0.0
29	6.0858086853010074E-5	0.0	0.0	2.2880206333257664	0.0
30	6.0858086853010074E-5	0.0	0.0	2.3959220213161534	0.0
31	6.0858086853010074E-5	0.0	0.0	2.4945121220180297	0.0
32	6.0858086853010074E-5	0.0	0.0	2.5788614303963016	0.0
33	6.0858086853010074E-5	0.0	0.0	2.654568890441446	0.0
34	6.0858086853010074E-5	0.0	0.0	2.73611872682448	0.0
35	6.0858086853010074E-5	0.0	0.0	2.877796353018287	0.0
36	6.0858086853010074E-5	0.0	0.0	2.97955107423652	0.0
37	6.0858086853010074E-5	0.0	0.0	3.081975234410136	0.0
38	6.0858086853010074E-5	0.0	0.0	3.1760009785980365	0.0
39	6.0858086853010074E-5	0.0	0.0	3.2719133234783806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	40	6.8175723E-9	45.000004	35
ACGCGAG	30	2.1660726E-6	45.000004	1
AGTTTCG	30	2.1660726E-6	45.000004	1
CGAAATT	40	6.8175723E-9	45.000004	37
GTACAAT	20	7.034172E-4	45.000004	13
CCCTAGA	20	7.034172E-4	45.000004	44
TAGACCG	40	6.8175723E-9	45.000004	1
GGCACGA	40	6.8175723E-9	45.000004	33
ACACCGT	30	2.1660726E-6	45.000004	35
GGCGTTA	35	1.2123564E-7	45.000004	20
CGACCTT	20	7.034172E-4	45.000004	23
ATTCGGT	30	2.1660726E-6	45.000004	30
TACGGTG	35	1.2123564E-7	45.000004	4
ACCTCGT	30	2.1660726E-6	45.000004	37
CGTTATC	35	1.2123564E-7	45.000004	22
CCGTATG	60	0.0	45.000004	1
CCCGTAC	30	2.1660726E-6	45.000004	4
CCGTAGA	20	7.034172E-4	45.000004	33
TACGCGG	20	7.034172E-4	45.000004	2
CCGTAAG	80	0.0	45.000004	1
>>END_MODULE