FastQCFastQC Report
Thu 26 May 2016
SRR1545131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545131_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1671072
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84520.5057831140728826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG253.891569E-545.01
ATCTCGC406.8175723E-945.019
TCGTTAG750.045.01
ACGTTCA207.034207E-445.036
CTATCGC253.891569E-545.044
GCGAACG207.034207E-445.01
CGGTTGA207.034207E-445.025
CCGGATA207.034207E-445.020
GCCGGAT207.034207E-445.019
CGACCCG1400.045.032
CAATTCG253.891569E-545.011
CTAAGCG700.045.01
TAGCACG453.8562575E-1045.01
TACGATA406.8175723E-945.023
ATATACG950.045.01
CGTAGCT253.891569E-545.013
TCGCTAA406.8175723E-945.022
ACGCTAA253.891569E-545.025
TTACGTC207.034207E-445.026
TGTAGCG207.034207E-445.01
Produced by FastQC (version 0.11.5)