##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545131_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1671072
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.799432340437754	31.0	31.0	33.0	28.0	33.0
2	31.120520839317518	31.0	31.0	33.0	30.0	34.0
3	31.249363282970453	31.0	31.0	34.0	28.0	34.0
4	35.13408279236322	35.0	35.0	37.0	32.0	37.0
5	35.02381584994542	35.0	35.0	37.0	32.0	37.0
6	35.09687853066774	37.0	35.0	37.0	32.0	37.0
7	35.46397821278796	35.0	35.0	37.0	35.0	37.0
8	35.53203093583041	37.0	35.0	37.0	35.0	37.0
9	37.3809219471094	39.0	37.0	39.0	35.0	39.0
10	36.84816213783727	39.0	37.0	39.0	32.0	39.0
11	36.47141415809732	38.0	35.0	39.0	32.0	39.0
12	35.222852755596406	35.0	35.0	39.0	30.0	39.0
13	34.76298208575094	35.0	33.0	39.0	29.0	39.0
14	35.441385529767715	36.0	33.0	40.0	27.0	41.0
15	35.881893179946765	36.0	34.0	40.0	30.0	41.0
16	36.090471864767046	36.0	34.0	40.0	31.0	41.0
17	35.9474636640432	36.0	34.0	40.0	31.0	41.0
18	36.02253463644894	36.0	35.0	40.0	31.0	41.0
19	35.8692462084219	36.0	35.0	40.0	31.0	41.0
20	35.58482100112981	36.0	34.0	40.0	30.0	41.0
21	35.45050183355355	35.0	34.0	40.0	30.0	41.0
22	35.27102063824898	35.0	34.0	40.0	29.0	41.0
23	35.19991538365792	35.0	34.0	40.0	29.0	41.0
24	35.268345110204706	35.0	34.0	40.0	30.0	41.0
25	35.17392129124298	35.0	34.0	39.0	30.0	41.0
26	34.92148872101262	35.0	34.0	39.0	29.0	41.0
27	34.920469016296124	35.0	34.0	39.0	29.0	41.0
28	34.99251079546543	36.0	34.0	39.0	29.0	41.0
29	34.97686036268934	36.0	34.0	39.0	29.0	41.0
30	34.93495193504529	36.0	34.0	39.0	29.0	41.0
31	34.68992060186515	35.0	34.0	39.0	28.0	41.0
32	34.517951350989065	35.0	34.0	40.0	27.0	41.0
33	34.24251917332108	35.0	33.0	40.0	25.0	41.0
34	33.98352255318741	35.0	33.0	40.0	24.0	41.0
35	33.74208950900979	35.0	33.0	40.0	23.0	41.0
36	33.48497910323433	35.0	33.0	39.0	22.0	41.0
37	33.5123070699527	35.0	33.0	39.0	23.0	41.0
38	33.534867438386854	35.0	33.0	39.0	23.0	41.0
39	33.53487342256946	35.0	33.0	39.0	23.0	41.0
40	33.22305023362249	35.0	32.0	39.0	22.0	41.0
41	33.27837280500182	35.0	32.0	39.0	23.0	41.0
42	33.25572387066506	35.0	32.0	39.0	23.0	41.0
43	33.20944579288026	35.0	32.0	39.0	23.0	41.0
44	33.185179333984415	35.0	32.0	39.0	23.0	41.0
45	33.265876036460426	35.0	32.0	39.0	23.0	41.0
46	33.271674110989835	35.0	33.0	39.0	23.0	41.0
47	33.18782793320695	35.0	32.0	39.0	23.0	41.0
48	33.24854344995308	35.0	32.0	39.0	23.0	41.0
49	33.28039486030524	35.0	32.0	39.0	23.0	41.0
50	33.04822951973344	35.0	32.0	39.0	22.0	41.0
51	32.70731303019858	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	2.0
13	4.0
14	23.0
15	52.0
16	152.0
17	463.0
18	1127.0
19	2314.0
20	4180.0
21	6540.0
22	9825.0
23	14015.0
24	20167.0
25	28083.0
26	35682.0
27	40901.0
28	43319.0
29	47737.0
30	56510.0
31	69147.0
32	85699.0
33	113331.0
34	197926.0
35	287248.0
36	97109.0
37	117177.0
38	166389.0
39	225859.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.724050190536374	24.91412697956761	29.596271136132973	14.765551693763046
2	30.358117424024815	26.42507324639513	31.679065893031538	11.537743436548514
3	26.695797667604985	26.066321499013807	34.98131738189617	12.256563451485034
4	23.266322456483024	28.04164033626319	35.01069971850405	13.681337488749737
5	21.232538155148312	31.45579603990732	33.989439114532466	13.322226690411904
6	18.40136152122709	41.77731420309837	30.57061574845369	9.25070852722085
7	85.93459767143486	4.048598743800387	8.730563374887497	1.2862402098772525
8	88.32097001206411	2.6021619654928094	7.642279925700389	1.4345880967426898
9	84.75284128990252	3.941003140499033	9.287271882958963	2.0188836866394744
10	56.116014151395035	22.2911999004232	14.567594933072902	7.025191015108864
11	50.56113680320178	15.962866950077556	22.967891269795675	10.508104976924992
12	48.174704620746446	19.93726182953218	22.53421755615557	9.353815993565808
13	17.76781610846211	49.71299860209494	20.982159954807454	11.53702533463549
14	9.8481693188564	50.06115834625917	26.348834759962468	13.741837574921966
15	9.013196319488328	23.45709819804293	54.99224449933935	12.537460983129392
16	8.642117155933436	17.53628808333812	54.877946611516435	18.943648149212002
17	9.558115987820992	17.537903812642426	34.022950537140225	38.88102966239636
18	14.36425240803508	21.518582083836005	44.63805269910572	19.47911280902319
19	30.037245552555486	20.508751268646712	28.70899638076636	20.745006798031444
20	31.34616581451906	19.91541956301105	30.840741751402696	17.897672871067194
21	14.997199402539207	34.26985791156814	31.082263361482926	19.65067932440972
22	17.15665153865303	26.24225646770456	22.016884969648228	34.58420702399418
23	12.448176978610137	35.518637138316	25.477298404856285	26.555887478217578
24	11.030524118649586	20.066699699354665	55.65750607992953	13.245270102066216
25	9.843142605465234	25.671604814155224	46.770097278872484	17.715155301507057
26	9.870011585377531	38.7128142892706	32.75795417552326	18.65921994982861
27	8.468695543938262	47.294969935466575	27.171121292200457	17.065213228394708
28	8.284801612378162	31.953620191110854	43.36820914957584	16.393369046935142
29	8.568152658892018	22.219329867294764	44.71842027153827	24.494097202274947
30	14.148941517780203	40.11371143792727	31.089205013308824	14.648142030983704
31	29.81044503169223	30.49024817602114	26.492694509871505	13.20661228241512
32	33.51315802071964	20.930516458895845	29.754074031519888	15.802251488864632
33	33.805964075754964	26.912544761685915	21.436598782099157	17.844892380459967
34	18.957890503820302	24.166223837153638	25.88583855154057	30.990047107485495
35	18.309983052794855	22.915409988318874	36.91654219566841	21.858064763217865
36	35.27998793588786	27.210257846460234	24.047796863330845	13.461957354321058
37	15.694177150954596	43.63570211217709	28.7657862737213	11.90433446314701
38	16.43112923919496	40.76287556730051	26.856353286991823	15.949641906512705
39	18.804097010781103	40.932467302426225	26.415438712395396	13.847996974397272
40	27.880007564006814	23.85785890733613	26.00211121962429	22.260022309032763
41	14.008373068305854	21.93867170295475	30.01294977116486	34.04000545757454
42	14.357490281687443	19.772996016928055	28.517323011815172	37.35219068956933
43	15.171997376534344	21.547126634878687	29.277374044924454	34.00350194366251
44	11.433738342812278	28.8788873250225	35.764587043526554	23.92278728863867
45	10.54789979127171	48.67210987916739	22.460013691809806	18.319976637751097
46	16.188530476245187	42.191180272304244	24.758179180789337	16.86211007066123
47	15.718831983301737	28.394108691905558	28.490094980946363	27.396964343846346
48	16.214621512418375	24.464595182014897	41.030727580858276	18.290055724708452
49	20.97838991976408	26.51453677639264	38.7274755366615	13.779597767181784
50	17.737057409854273	41.74110989831677	27.249932977154785	13.271899714674174
51	14.289629650906724	38.486013768407346	26.251352425269523	20.9730041554164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2252.0
1	3147.0
2	4042.0
3	10178.0
4	16314.0
5	12460.0
6	8606.0
7	8852.0
8	9098.0
9	9573.0
10	10048.0
11	10240.5
12	10433.0
13	10454.0
14	10475.0
15	10511.0
16	10547.0
17	10003.5
18	9460.0
19	9219.5
20	8979.0
21	7981.5
22	6984.0
23	7662.5
24	8341.0
25	10219.0
26	16166.0
27	20235.0
28	23508.5
29	26782.0
30	32301.0
31	37820.0
32	48157.0
33	58494.0
34	65526.0
35	72558.0
36	73245.5
37	73933.0
38	87697.0
39	101461.0
40	140460.0
41	179459.0
42	209828.5
43	240198.0
44	228112.5
45	216027.0
46	187834.5
47	159642.0
48	134230.0
49	108818.0
50	90967.5
51	73117.0
52	61495.5
53	49874.0
54	44433.5
55	38993.0
56	33943.0
57	28893.0
58	23496.5
59	18100.0
60	14763.0
61	11426.0
62	9924.0
63	8422.0
64	7214.0
65	6006.0
66	5137.0
67	4268.0
68	3692.5
69	3117.0
70	2689.5
71	2262.0
72	1933.5
73	1605.0
74	1286.5
75	732.0
76	496.0
77	353.5
78	211.0
79	168.0
80	125.0
81	84.0
82	43.0
83	31.5
84	20.0
85	17.5
86	15.0
87	9.5
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1671072.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.043564671240432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34308056400633	15.300186506073347
2	7.222028015922718	2.75066315157469
3	2.7481686786516857	1.5700478387814223
4	1.503993142089961	1.145655626659691
5	1.0640744975654612	1.0131885754702763
6	0.7738512807932167	0.8842132147024718
7	0.6117553579577075	0.815500190557181
8	0.48937786399171607	0.7455599201279799
9	0.4105638189011033	0.7036738773223143
>10	3.2657369604662896	12.166754500803737
>50	0.43303094433799355	5.8912247481781925
>100	1.0813146874880648	49.37460551969943
>500	0.047974265177517725	5.990818487584849
>1k	0.0047343024846234595	1.139897590266541
>5k	3.15620165641564E-4	0.5080102521978804
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8452	0.5057831140728826	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0290831274774516	0.0
2	0.0	0.0	0.0	0.10717671051875682	0.0
3	0.0	0.0	0.0	0.14930535608280193	0.0
4	0.0	0.0	0.0	0.22596273529806016	0.0
5	0.0	0.0	0.0	0.4068047337278107	0.0
6	0.0	0.0	0.0	0.6075740602439632	0.0
7	0.0	0.0	0.0	0.7614872369353325	0.0
8	0.0	0.0	0.0	1.152852779533138	0.0
9	0.0	0.0	0.0	1.3258554987457154	0.0
10	0.0	0.0	0.0	1.552536335956799	0.0
11	0.0	0.0	0.0	1.7245815859520117	0.0
12	0.0	0.0	0.0	1.895130790295092	0.0
13	0.0	0.0	0.0	1.9594607533367803	0.0
14	0.0	0.0	0.0	1.992313915857605	0.0
15	0.0	0.0	0.0	2.029056797073974	0.0
16	0.0	0.0	0.0	2.108646425767412	0.0
17	0.0	0.0	0.0	2.2061287604603512	0.0
18	0.0	0.0	0.0	2.370394573064476	0.0
19	0.0	0.0	0.0	2.433826908714885	0.0
20	5.984182608529136E-5	0.0	0.0	2.5037820034085905	0.0
21	5.984182608529136E-5	0.0	0.0	2.598391930449436	0.0
22	5.984182608529136E-5	0.0	0.0	2.707244212098581	0.0
23	5.984182608529136E-5	0.0	0.0	2.8332710978342046	0.0
24	5.984182608529136E-5	0.0	0.0	2.936677773309588	0.0
25	5.984182608529136E-5	0.0	0.0	3.0234484211332604	0.0
26	5.984182608529136E-5	0.0	0.0	3.113630053043795	0.0
27	5.984182608529136E-5	0.0	0.0	3.200161333563126	0.0
28	5.984182608529136E-5	0.0	0.0	3.2878296087780776	0.0
29	5.984182608529136E-5	0.0	0.0	3.3890221366883053	0.0
30	5.984182608529136E-5	0.0	0.0	3.534138564945137	0.0
31	5.984182608529136E-5	0.0	0.0	3.6562158901591313	0.0
32	5.984182608529136E-5	0.0	0.0	3.7563911070259093	0.0
33	5.984182608529136E-5	0.0	0.0	3.875296815457383	0.0
34	5.984182608529136E-5	0.0	0.0	4.012334597192701	0.0
35	5.984182608529136E-5	0.0	0.0	4.205683537274276	0.0
36	5.984182608529136E-5	0.0	0.0	4.352834587618008	0.0
37	5.984182608529136E-5	0.0	0.0	4.485563757875184	0.0
38	5.984182608529136E-5	0.0	0.0	4.629124298653799	0.0
39	5.984182608529136E-5	0.0	0.0	4.792432642040558	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.891569E-5	45.0	1
ATCTCGC	40	6.8175723E-9	45.0	19
TCGTTAG	75	0.0	45.0	1
ACGTTCA	20	7.034207E-4	45.0	36
CTATCGC	25	3.891569E-5	45.0	44
GCGAACG	20	7.034207E-4	45.0	1
CGGTTGA	20	7.034207E-4	45.0	25
CCGGATA	20	7.034207E-4	45.0	20
GCCGGAT	20	7.034207E-4	45.0	19
CGACCCG	140	0.0	45.0	32
CAATTCG	25	3.891569E-5	45.0	11
CTAAGCG	70	0.0	45.0	1
TAGCACG	45	3.8562575E-10	45.0	1
TACGATA	40	6.8175723E-9	45.0	23
ATATACG	95	0.0	45.0	1
CGTAGCT	25	3.891569E-5	45.0	13
TCGCTAA	40	6.8175723E-9	45.0	22
ACGCTAA	25	3.891569E-5	45.0	25
TTACGTC	20	7.034207E-4	45.0	26
TGTAGCG	20	7.034207E-4	45.0	1
>>END_MODULE