FastQCFastQC Report
Thu 26 May 2016
SRR1545130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545130_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2285177
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105620.46219614498132966No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG50130.21937031573484242No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG48080.21039945702236632No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG40000.1750411456092898No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26410.11557091638853359No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA351.2126293E-745.00000425
CGCATTA302.1664473E-645.00000424
ATTGTCG351.2126293E-745.0000041
GTTAGCG950.045.0000041
TGCGCGT302.1664473E-645.00000437
AACGACC302.1664473E-645.00000431
ACATGCG351.2126293E-745.0000041
CCGACAA302.1664473E-645.00000418
ATAGTCG302.1664473E-645.0000041
CGCAAAA351.2126293E-745.00000443
CGTCCAA453.8562575E-1045.00000444
GCATCGA351.2126293E-745.0000049
TGCGTAG1150.045.0000041
ACACGGC207.0347526E-445.09
TCACCGC253.8920203E-545.027
CAACGAC207.0347526E-445.023
CTATACG406.8193913E-945.01
CGTCTTA551.8189894E-1245.027
TTACTCG207.0347526E-445.025
GATCACG551.8189894E-1245.01