FastQCFastQC Report
Thu 26 May 2016
SRR1545128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545128_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1921811
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.34816118754653813No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG29860.15537427978089416No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT26350.13711025693993845No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21870.11379891154749348No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20510.10672225312478698No Hit
ATTGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC19340.10063424551113506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG1250.045.0000041
TATTGCG750.045.0000041
TTGCGAG750.045.0000041
TTACCCG750.045.00000445
TATCCGA750.045.00000430
AACGTGC207.034472E-445.044
CGGGTGC502.1827873E-1145.06
AACGTAG207.034472E-445.01
TACCCGG351.212502E-745.02
TACCCGC406.8193913E-945.045
CGAACGG1000.045.02
CACGGTA207.034472E-445.030
GTCGAAG453.8562575E-1045.01
ACGTTAC207.034472E-445.045
GCGAAAG700.045.01
TCCGAAC502.1827873E-1145.039
GCAACCG207.034472E-445.030
ATTACGT551.8189894E-1245.031
ATTACGC207.034472E-445.033
TTACTCG253.8917886E-545.020