FastQCFastQC Report
Thu 26 May 2016
SRR1545126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545126_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2192489
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126580.5773347095470035No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30520.13920252279486922No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27580.1257931054614185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTC302.1664073E-645.00000418
CGTTAAG453.8562575E-1045.00000418
CTTACCG302.1664073E-645.0000041
ATCTCGC750.045.015
ACCGGTA207.034689E-445.035
ACGGGTC253.891969E-545.05
TGATTCG351.2126111E-745.015
GCGATTC850.045.09
TTACGCA351.2126111E-745.013
TCGAACA351.2126111E-745.043
TACGTAG502.1827873E-1145.01
CTAGCGA406.8193913E-945.017
TAATCCG207.034689E-445.024
ACTTACG650.044.9999961
TAATGCG1450.043.448281
CGTTTTT105550.043.4012341
TACGGGA6500.042.9230774
CGACGGT1800.042.50000428
CGGTCTA1800.042.50000431
TTGCGAG4900.042.24491
Produced by FastQC (version 0.11.5)