##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545126_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2192489
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.830509069828857	31.0	31.0	33.0	28.0	33.0
2	31.143481221570553	31.0	31.0	33.0	30.0	34.0
3	31.257841658498627	31.0	31.0	34.0	28.0	34.0
4	35.14862104211241	35.0	35.0	37.0	32.0	37.0
5	35.04296897270636	35.0	35.0	37.0	32.0	37.0
6	35.12699721640565	37.0	35.0	37.0	32.0	37.0
7	35.508653407155066	36.0	35.0	37.0	35.0	37.0
8	35.58410007986357	37.0	35.0	37.0	35.0	37.0
9	37.40166814976039	39.0	37.0	39.0	35.0	39.0
10	36.85992449677057	39.0	37.0	39.0	32.0	39.0
11	36.48428658022914	38.0	35.0	39.0	32.0	39.0
12	35.55324108809668	37.0	35.0	39.0	31.0	39.0
13	35.21743871919084	37.0	35.0	39.0	30.0	39.0
14	36.000417790009436	38.0	35.0	40.0	30.0	41.0
15	36.31773067048455	38.0	35.0	40.0	31.0	41.0
16	36.423954692589106	38.0	35.0	40.0	32.0	41.0
17	36.25769479345164	37.0	35.0	40.0	31.0	41.0
18	36.31660364088486	37.0	35.0	40.0	31.0	41.0
19	36.167230029432304	37.0	35.0	40.0	31.0	41.0
20	35.93230844031601	36.0	34.0	40.0	31.0	41.0
21	35.84478599436531	36.0	34.0	40.0	30.0	41.0
22	35.65726167839382	36.0	34.0	40.0	30.0	41.0
23	35.55480643232418	36.0	34.0	40.0	30.0	41.0
24	35.636670469042265	36.0	34.0	40.0	30.0	41.0
25	35.52956708106631	35.0	34.0	40.0	30.0	41.0
26	35.25834793241836	35.0	34.0	40.0	29.0	41.0
27	35.27882739662548	35.0	34.0	40.0	29.0	41.0
28	35.30269068624746	36.0	34.0	40.0	29.0	41.0
29	35.26047838780491	36.0	34.0	40.0	29.0	41.0
30	35.23382466229021	36.0	34.0	40.0	29.0	41.0
31	35.06785347611778	36.0	34.0	40.0	29.0	41.0
32	34.934272418242465	35.0	34.0	40.0	28.0	41.0
33	34.67216528794443	35.0	33.0	40.0	27.0	41.0
34	34.39490962098328	35.0	34.0	40.0	25.0	41.0
35	34.181636943218415	35.0	33.0	40.0	24.0	41.0
36	33.92115353828457	35.0	33.0	40.0	23.0	41.0
37	33.94862368750767	35.0	33.0	40.0	23.0	41.0
38	33.979297501606624	35.0	33.0	40.0	23.0	41.0
39	33.97299188274149	35.0	33.0	40.0	23.0	41.0
40	33.7039529046668	35.0	33.0	40.0	23.0	41.0
41	33.738823775170594	35.0	33.0	40.0	23.0	41.0
42	33.7095720890732	35.0	33.0	40.0	23.0	41.0
43	33.66144277120661	35.0	33.0	40.0	23.0	41.0
44	33.620128538843296	35.0	33.0	40.0	23.0	41.0
45	33.66840882668055	35.0	33.0	40.0	23.0	41.0
46	33.64677542281854	35.0	33.0	40.0	23.0	41.0
47	33.582935193745556	35.0	33.0	40.0	23.0	41.0
48	33.64899025719171	35.0	33.0	40.0	23.0	41.0
49	33.63337877635874	36.0	33.0	39.0	23.0	41.0
50	33.49465835404419	35.0	33.0	39.0	23.0	41.0
51	33.182869332525726	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	3.0
12	5.0
13	9.0
14	17.0
15	55.0
16	179.0
17	538.0
18	1394.0
19	2692.0
20	4794.0
21	7883.0
22	11833.0
23	17286.0
24	24474.0
25	34076.0
26	43367.0
27	49129.0
28	52592.0
29	59168.0
30	69432.0
31	84776.0
32	106192.0
33	139664.0
34	235434.0
35	330859.0
36	144403.0
37	177789.0
38	251786.0
39	342555.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.30565808996077	23.4730026011533	29.339941956379256	14.881397352506673
2	31.211650320708568	26.314202716638484	30.485033220235085	11.989113742417864
3	27.197536680913792	25.857096660462155	35.07096272774915	11.874403930874909
4	25.08122047590661	27.766615932850748	33.75788886512087	13.394274726121774
5	21.22555688990914	32.31313817309916	34.18384311164161	12.277461825350093
6	18.960596837658024	41.508030370961954	30.44485057849777	9.086522212882254
7	86.18606524365686	4.176623007002544	8.25249294295205	1.3848188063885385
8	88.24386348118509	2.8648262317393614	7.729023954054045	1.1622863330215112
9	83.55677041025064	5.242078751592368	9.23279432644816	1.9683565117088389
10	49.37995127911702	27.763605655490174	15.179870913833545	7.676572151559255
11	44.73244791650038	18.20072985542915	26.281682599091717	10.785139628978754
12	43.99661754289303	21.290460294213563	25.078301419072112	9.63462074382129
13	18.639728637179022	46.56096336173181	22.559885135113564	12.23942286597561
14	11.084571005829448	45.88173532455579	26.624306895040295	16.409386774574468
15	9.69715241444769	25.504985429801476	50.919434487470625	13.878427668280205
16	10.400417060245228	19.643929798507543	48.761658553361045	21.193994587886188
17	10.739392535150689	21.4857178302833	31.87354645792978	35.90134317663624
18	15.86169873600278	23.946665182812776	41.93735977694757	18.254276304236875
19	27.418427184811417	24.555744635434888	27.93988932213571	20.085938857617986
20	28.81277853617509	21.942230953040127	32.47710706872417	16.7678834420606
21	16.059464836539657	33.28969951502607	31.38127488895041	19.269560759483856
22	19.857887542423246	25.99835164509377	23.42889747679464	30.714863335688342
23	13.869031954094183	32.445453546175145	27.323785889005602	26.36172861072507
24	11.28397907583573	22.195732795010603	52.976457350527184	13.543830778626484
25	11.380855274530454	29.10071612673997	42.78616677210239	16.73226182662718
26	12.506197294490418	38.083703042523815	29.819989974864185	19.590109688121583
27	10.69031589212078	44.878035876120705	27.837813553454545	16.593834678303974
28	10.519231795461687	33.41084037365752	39.83039367586337	16.239534155017427
29	10.730863415962407	24.38566396456265	39.24858916053855	25.634883458936397
30	13.697126872700387	40.60093345964336	30.43495315141832	15.266986516237937
31	26.432743790276714	32.62990144990465	27.601506780649753	13.33584797916888
32	29.211731506976772	24.167327635395207	30.372512701318	16.24842815631002
33	29.387695901781036	30.21565900672706	22.79277113819043	17.603873953301477
34	17.266950940232768	27.171037118088165	27.162462388636843	28.399549553042224
35	19.54769214349536	26.307224346393525	33.514330060492895	20.63075344961822
36	31.204717560726646	30.2631392905506	24.03492104179314	14.497222106929614
37	15.023290880820838	41.794918925476935	30.45105357427107	12.730736619431157
38	15.133211614744704	38.129769408193155	29.89433470361767	16.842684273444473
39	18.28998914019637	39.226741844542886	28.403608866452696	14.079660148808044
40	24.55998638989751	27.482692045433293	28.26714295944016	19.690178605229033
41	13.660091339112762	26.250257127857886	31.38018024263748	28.709471290391882
42	15.284181585403623	23.576401067462598	28.26103118419294	32.87838616294084
43	16.144619197633375	25.497550956926123	30.89402044890533	27.463809396535172
44	12.872219655377975	31.579588312643757	33.0291736925476	22.519018339430666
45	12.076229344822256	48.63317444238033	23.08412949848323	16.20646671431419
46	16.904714231177444	41.27245336236579	25.827632430538987	15.995199975917782
47	15.701013779316566	32.009875534153196	28.220529270614357	24.068581415915887
48	16.378326185444944	28.793531005172664	38.00005382011039	16.82808898927201
49	19.937659892478365	29.713991723561666	36.160546301486576	14.18780208247339
50	17.706725096454303	40.675916732079386	27.39197323224883	14.225384939217484
51	15.389997395653982	34.74494056754674	28.29993673856516	21.565125298234108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2139.0
1	3123.5
2	4108.0
3	13652.5
4	23197.0
5	18138.0
6	13079.0
7	13455.5
8	13832.0
9	14491.5
10	15151.0
11	15223.5
12	15296.0
13	14680.5
14	14065.0
15	13179.0
16	12293.0
17	11318.0
18	10343.0
19	10392.5
20	10442.0
21	9699.0
22	8956.0
23	10627.0
24	12298.0
25	15262.5
26	23346.5
27	28466.0
28	37440.0
29	46414.0
30	56777.0
31	67140.0
32	78043.0
33	88946.0
34	109125.0
35	129304.0
36	132510.5
37	135717.0
38	144448.0
39	153179.0
40	185973.5
41	218768.0
42	242837.5
43	266907.0
44	264906.0
45	262905.0
46	232191.5
47	201478.0
48	166796.5
49	132115.0
50	113264.5
51	94414.0
52	76437.0
53	58460.0
54	46011.5
55	33563.0
56	28195.5
57	22828.0
58	21295.0
59	19762.0
60	18277.5
61	16793.0
62	14740.0
63	12687.0
64	10841.5
65	8996.0
66	7216.5
67	5437.0
68	5171.5
69	4906.0
70	4380.0
71	3854.0
72	3330.0
73	2806.0
74	2174.0
75	1121.0
76	700.0
77	579.0
78	458.0
79	359.0
80	260.0
81	200.0
82	140.0
83	107.0
84	74.0
85	47.0
86	20.0
87	14.0
88	8.0
89	7.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2192489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.553997154472473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89653196052986	14.824000266478901
2	7.269333355445526	2.697503807836963
3	2.7342533013291828	1.521939839174059
4	1.559302380890348	1.1572516775200667
5	1.0384892541033188	0.9634063332791611
6	0.7748942827979836	0.8626431790830466
7	0.5982239313818708	0.7769611584417597
8	0.491797081379946	0.7299841318801108
9	0.4305599356765266	0.7189747039234913
>10	3.5209004523643928	13.24519882523031
>50	0.5631432881506503	7.531163784745895
>100	1.0703237936438652	47.660168561517466
>500	0.04756446974128022	5.517065755273457
>1k	0.004436064535456186	1.21493876853521
>5k	0.0	0.0
>10k+	2.464480297475659E-4	0.5787992070801162
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12658	0.5773347095470035	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3052	0.13920252279486922	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2758	0.1257931054614185	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01979485415890342	0.0
2	0.0	0.0	0.0	0.07060468718429146	0.0
3	0.0	0.0	0.0	0.10481238446350244	0.0
4	0.0	0.0	0.0	0.15671686380182523	0.0
5	0.0	0.0	0.0	0.2721108292903636	0.0
6	0.0	0.0	0.0	0.40980821340494755	0.0
7	0.0	0.0	0.0	0.5074597865713352	0.0
8	0.0	0.0	0.0	0.7797986671768935	0.0
9	0.0	0.0	0.0	0.8970170431869897	0.0
10	0.0	0.0	0.0	1.0870750092702859	0.0
11	0.0	0.0	0.0	1.2309753891581667	0.0
12	0.0	0.0	0.0	1.3616031825017139	0.0
13	0.0	0.0	0.0	1.4138725439443482	0.0
14	0.0	0.0	0.0	1.4326183620533557	0.0
15	0.0	0.0	0.0	1.4619457611873994	0.0
16	0.0	0.0	0.0	1.5328241099499245	0.0
17	0.0	0.0	0.0	1.6215360715606784	0.0
18	0.0	0.0	0.0	1.7572722143645874	0.0
19	0.0	0.0	0.0	1.8188004592041283	0.0
20	0.0	0.0	0.0	1.8809672477262143	0.0
21	0.0	0.0	0.0	1.966349660135125	0.0
22	0.0	0.0	0.0	2.0616751098865262	0.0
23	0.0	0.0	0.0	2.1772059061641813	0.0
24	0.0	0.0	0.0	2.270433283815791	0.0
25	0.0	0.0	0.0	2.3410835812631214	0.0
26	0.0	0.0	0.0	2.4166141768556195	0.0
27	0.0	0.0	0.0	2.4919167211329225	0.0
28	0.0	0.0	0.0	2.5725556661857825	0.0
29	0.0	0.0	0.0	2.66847404935669	0.0
30	0.0	0.0	0.0	2.8172547273897384	0.0
31	0.0	0.0	0.0	2.932466251826121	0.0
32	0.0	0.0	0.0	3.037324246552662	0.0
33	0.0	0.0	0.0	3.1451012981136963	0.0
34	0.0	0.0	0.0	3.261316248336936	0.0
35	0.0	0.0	0.0	3.4218643742340324	0.0
36	0.0	0.0	0.0	3.5448296433870365	0.0
37	0.0	0.0	0.0	3.6692088306942474	0.0
38	4.561026303894797E-5	0.0	0.0	3.79700878772938	0.0
39	4.561026303894797E-5	0.0	0.0	3.9258121705513687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTC	30	2.1664073E-6	45.000004	18
CGTTAAG	45	3.8562575E-10	45.000004	18
CTTACCG	30	2.1664073E-6	45.000004	1
ATCTCGC	75	0.0	45.0	15
ACCGGTA	20	7.034689E-4	45.0	35
ACGGGTC	25	3.891969E-5	45.0	5
TGATTCG	35	1.2126111E-7	45.0	15
GCGATTC	85	0.0	45.0	9
TTACGCA	35	1.2126111E-7	45.0	13
TCGAACA	35	1.2126111E-7	45.0	43
TACGTAG	50	2.1827873E-11	45.0	1
CTAGCGA	40	6.8193913E-9	45.0	17
TAATCCG	20	7.034689E-4	45.0	24
ACTTACG	65	0.0	44.999996	1
TAATGCG	145	0.0	43.44828	1
CGTTTTT	10555	0.0	43.401234	1
TACGGGA	650	0.0	42.923077	4
CGACGGT	180	0.0	42.500004	28
CGGTCTA	180	0.0	42.500004	31
TTGCGAG	490	0.0	42.2449	1
>>END_MODULE