FastQCFastQC Report
Thu 26 May 2016
SRR1545125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545125_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1956529
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.4044918322192005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC207.0345023E-445.00000420
CGAACGG351.2125201E-745.0000042
CGCGGGC207.0345023E-445.0000044
ACGCAAA207.0345023E-445.00000419
GCGCGAC750.045.0000049
GCGCCCT406.8193913E-945.00000421
CGTTCTA351.2125201E-745.00000440
TACGCAT406.8193913E-945.00000445
TCTCGAG700.045.0000041
AGCGCAC207.0345023E-445.00000444
ACTACGG406.8193913E-945.0000042
GTCCGTC207.0345023E-445.00000412
CGAATGT406.8193913E-945.00000441
TCCGTCG351.2125201E-745.0000041
AACCTCG207.0345023E-445.00000425
CGTCGCA207.0345023E-445.00000439
CGGTCCC207.0345023E-445.00000419
CACGTCA207.0345023E-445.00000434
ACTCACG207.0345023E-445.0000042
AAGGCGC406.8193913E-945.00000418