FastQCFastQC Report
Thu 26 May 2016
SRR1545124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545124_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1928597
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101470.5261337645967509No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG33210.1721977167858293No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31620.16395338165516177No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22240.11531698950065775No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20830.10800597532817897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTG207.034476E-445.0000041
TATCACG351.212502E-745.0000041
GGTAATC1250.045.0000048
ACGCATG406.8193913E-945.0000041
CAGTTCG207.034476E-445.0000041
GTTAGTC207.034476E-445.00000435
TACGGCA207.034476E-445.00000436
ATCCGGT207.034476E-445.00000411
TGTTGCG207.034476E-445.0000041
GTTACGT351.212502E-745.00000429
TGATTCG351.212502E-745.00000415
CTCATCG207.034476E-445.00000421
CGTGTTA207.034476E-445.00000432
ATACGTT207.034476E-445.00000427
GATTCGT207.034476E-445.00000411
TGCGTAG207.034476E-445.0000041
AGTCGAT207.034476E-445.00000424
ATAGCCG253.891794E-545.015
TCGATTG950.045.01
CACGAAC253.891794E-545.042