FastQCFastQC Report
Thu 26 May 2016
SRR1545123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545123_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1085708
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34400.3168439396228083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14640.1348428859324975No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14490.1334612989864678No Hit
GGCTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12670.1166980440413076No Hit
TAGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12640.11642172665210168No Hit
TGGTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA12150.11190854262840469No Hit
GATTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA10970.10104005865297115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG453.8562575E-1045.01
GTTGACG502.1827873E-1145.01
GTGCACG351.2118471E-745.01
CGGGTAG453.8562575E-1045.06
CGGGTAC207.0331135E-445.06
CTCACTA850.045.045
TCGTTAG551.8189894E-1245.01
CCGGGTC207.0331135E-445.04
GTACCGG351.2118471E-745.02
AGTCCCA253.8906626E-545.015
CTACTTA253.8906626E-545.028
GTCGATA253.8906626E-545.09
CTACTAT207.0331135E-445.038
CTGTACG502.1827873E-1145.01
GTACACG551.8189894E-1245.01
TCCGATT207.0331135E-445.06
CGACGAA351.2118471E-745.035
TTAGCCG253.8906626E-545.01
CGTGCAT351.2118471E-745.029
GGCACGG453.8562575E-1045.02