##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545122_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2221889
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92810261898772	31.0	31.0	33.0	30.0	33.0
2	31.254379944272642	31.0	31.0	33.0	30.0	34.0
3	31.408895313852312	31.0	31.0	34.0	30.0	34.0
4	35.22376770396721	37.0	35.0	37.0	33.0	37.0
5	35.10687887648753	37.0	35.0	37.0	32.0	37.0
6	35.18398803900645	37.0	35.0	37.0	32.0	37.0
7	35.59628541299768	36.0	35.0	37.0	35.0	37.0
8	35.67175542972669	37.0	35.0	37.0	35.0	37.0
9	37.491840501483196	39.0	37.0	39.0	35.0	39.0
10	37.02273695940706	39.0	37.0	39.0	33.0	39.0
11	36.57756575598511	39.0	35.0	39.0	32.0	39.0
12	35.14262323635429	35.0	35.0	39.0	31.0	39.0
13	34.642821491082586	35.0	33.0	39.0	30.0	39.0
14	35.25400773846038	35.0	33.0	40.0	27.0	41.0
15	35.70890760069472	35.0	34.0	40.0	30.0	41.0
16	35.93845057066307	35.0	34.0	40.0	32.0	41.0
17	35.79315888417468	35.0	34.0	40.0	31.0	41.0
18	35.906427818851434	36.0	35.0	40.0	31.0	41.0
19	35.78666125985592	36.0	35.0	40.0	31.0	41.0
20	35.50717565098886	35.0	34.0	40.0	31.0	41.0
21	35.35544799942751	35.0	34.0	40.0	30.0	41.0
22	35.19758457780744	35.0	34.0	40.0	30.0	41.0
23	35.13983641847095	35.0	34.0	39.0	30.0	41.0
24	35.22449636322967	35.0	34.0	39.0	30.0	41.0
25	35.12873685409127	35.0	34.0	39.0	30.0	41.0
26	34.882397815552444	35.0	34.0	39.0	29.0	41.0
27	34.86991249337838	35.0	34.0	39.0	29.0	41.0
28	34.98584222704194	35.0	34.0	39.0	29.0	41.0
29	34.98746967107718	36.0	34.0	39.0	29.0	41.0
30	34.94652073078358	36.0	34.0	39.0	29.0	41.0
31	34.686801635905304	35.0	34.0	39.0	29.0	41.0
32	34.53191361044588	35.0	34.0	39.0	29.0	41.0
33	34.29091147217525	35.0	33.0	39.0	27.0	41.0
34	34.10419242365393	35.0	34.0	39.0	26.0	41.0
35	33.86593569705777	35.0	33.0	39.0	24.0	41.0
36	33.57866437072239	35.0	33.0	39.0	23.0	41.0
37	33.56767687314713	35.0	33.0	39.0	23.0	41.0
38	33.62417474500301	35.0	33.0	39.0	23.0	41.0
39	33.65717639360022	35.0	33.0	39.0	23.0	41.0
40	33.2741469083289	35.0	32.0	39.0	23.0	41.0
41	33.408505105340545	35.0	33.0	39.0	23.0	41.0
42	33.39893261994636	35.0	33.0	39.0	23.0	41.0
43	33.34305539115591	35.0	33.0	39.0	23.0	41.0
44	33.30045200277782	35.0	33.0	39.0	23.0	41.0
45	33.36461722435279	35.0	33.0	39.0	23.0	41.0
46	33.389074791765026	35.0	33.0	39.0	23.0	41.0
47	33.3633192297185	35.0	33.0	39.0	23.0	41.0
48	33.409030784166085	35.0	33.0	39.0	23.0	41.0
49	33.42826216791208	35.0	33.0	38.0	24.0	41.0
50	33.19962158325641	35.0	32.0	38.0	23.0	41.0
51	32.91135740804334	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	4.0
13	2.0
14	10.0
15	45.0
16	159.0
17	518.0
18	1266.0
19	2692.0
20	4692.0
21	7737.0
22	11813.0
23	17069.0
24	24240.0
25	33368.0
26	42705.0
27	49655.0
28	54271.0
29	61788.0
30	73350.0
31	91286.0
32	116299.0
33	155794.0
34	289941.0
35	425080.0
36	119229.0
37	144437.0
38	207194.0
39	287094.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.205969335101795	25.67545003373256	30.081205676791235	13.037374954374409
2	29.500348577269165	26.569374077642944	31.008704755278053	12.921572589809843
3	28.68788674861796	25.42323221367044	32.93004285992684	12.95883817778476
4	25.886351658431185	28.925747415825004	31.62151664642113	13.566384279322685
5	21.764768627055627	33.50567917659253	31.630472989424764	13.099079206927078
6	19.90792519338275	42.201163064401506	28.715565899106572	9.175345843109174
7	85.62695976261641	5.329114100659394	7.5256684739876745	1.5182576627365274
8	88.1826229843165	3.600809941450721	6.733909749766978	1.4826573244658037
9	83.51893366410293	5.590063229981336	8.535709929703959	2.355293176211773
10	60.908083167070906	19.34579090134566	12.974050458866307	6.772075472717135
11	58.08665509393134	15.894763419774796	18.23254897071816	7.786032515575711
12	52.58674038172024	20.32441764642608	19.760033016950892	7.328808954902788
13	18.083981693054874	54.19712685917253	18.926688056874127	8.792203390898464
14	9.517532153946483	55.412804149982286	24.653076728855492	10.416586967215734
15	8.766504537355377	22.862798276601577	59.21997003450667	9.150727151536373
16	9.033754611503996	16.309635629862697	58.16874740367318	16.487862354960125
17	8.853457575963516	17.031993947492428	31.422721837139477	42.69182663940458
18	14.789442676929406	23.350626426432644	45.705208496013974	16.154722400623974
19	33.29387741691867	19.14978651048725	29.10442420841005	18.451911864184034
20	33.07811506335375	20.274910222787906	29.351196211871972	17.295778501986373
21	15.810825833333709	35.24802544141494	31.543699977811674	17.39744874743968
22	18.690627659617558	25.589532150345946	20.857927646250555	34.86191254378594
23	12.255652735127633	34.58120545175749	25.202429104244185	27.960712708870695
24	10.191733250400898	21.271764701116933	56.03853297802005	12.497969070462116
25	8.974435716635709	25.319086597035223	50.2518802694464	15.454597416882661
26	9.10765569297116	41.67084854373913	30.884846182685095	18.33664958060461
27	9.34097067855325	51.664147038848476	26.89418778345813	12.100694499140147
28	8.490883207937031	36.60151339693387	42.753845939198584	12.153757455930517
29	8.938160277133555	26.119351596771935	42.05039045604889	22.892097670045626
30	13.834219441205208	42.543079334746245	30.231033143419854	13.39166808062869
31	32.40625431783496	31.362367787049667	26.16363823755372	10.067739657561651
32	36.32773734421477	22.141340093947086	30.200563574508	11.33035898733015
33	37.32369168756855	27.29713320512411	20.4054748009464	14.973700306360938
34	20.35056656745679	26.4786404721388	24.359407693183595	28.811385267220817
35	18.566048979044407	24.73404386987829	36.00202350342434	20.697883647652965
36	39.23094267985485	25.6081649443334	23.490237361092294	11.670655014719458
37	15.958988050258135	45.433367733491636	28.23358862661456	10.374055589635665
38	16.449651625261207	43.588046027501825	23.019871829780875	16.942430517456092
39	20.057437612770034	41.17523422637224	26.575404981977048	12.191923178880673
40	27.990777217043693	24.464858505532906	25.61649119285437	21.92787308456903
41	12.886962400011882	25.26548355925971	27.276520114191122	34.57103392653729
42	16.05899304600725	21.270459505402837	26.076100111211677	36.59444733737824
43	17.137579780088025	20.559307868214837	30.085346297677333	32.217766054019805
44	11.274685639111585	31.209569875002757	36.300778301706345	21.214966184179318
45	10.625328267973783	53.231462057735556	22.324742595152145	13.818467079138516
46	17.158507918262345	43.225066598736475	25.023347250920274	14.5930782320809
47	17.74062520674975	30.17058907983252	25.50568457740238	26.583101136015348
48	18.048921435769294	27.51046519425588	39.0988928789872	15.341720490987623
49	21.904919642700424	26.69444783245248	38.93331305029189	12.46731947455521
50	18.36941449370333	45.640308764299206	24.182036096312643	11.808240645684819
51	16.65569252109354	39.96693804235945	24.32907314451802	19.04829629202899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3937.0
1	4614.5
2	5292.0
3	10522.0
4	15752.0
5	13088.5
6	10425.0
7	11087.0
8	11749.0
9	12596.5
10	13444.0
11	13661.0
12	13878.0
13	13232.5
14	12587.0
15	11751.0
16	10915.0
17	10532.0
18	10149.0
19	9632.5
20	9116.0
21	9714.5
22	10313.0
23	12291.0
24	14269.0
25	16876.5
26	22589.0
27	25694.0
28	37145.0
29	48596.0
30	53660.5
31	58725.0
32	67300.0
33	75875.0
34	81862.0
35	87849.0
36	93960.5
37	100072.0
38	116055.5
39	132039.0
40	184910.0
41	237781.0
42	292911.5
43	348042.0
44	329784.0
45	311526.0
46	268043.0
47	224560.0
48	189819.0
49	155078.0
50	130407.5
51	105737.0
52	81684.5
53	57632.0
54	41945.0
55	26258.0
56	21000.5
57	15743.0
58	13992.5
59	12242.0
60	11668.0
61	11094.0
62	9685.5
63	8277.0
64	7162.5
65	6048.0
66	4953.5
67	3859.0
68	3333.5
69	2808.0
70	2399.5
71	1991.0
72	1739.0
73	1487.0
74	1114.5
75	605.5
76	469.0
77	313.5
78	158.0
79	132.5
80	107.0
81	78.5
82	50.0
83	34.0
84	18.0
85	14.5
86	11.0
87	9.0
88	7.0
89	4.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2221889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.743329510159546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4019625998138	12.815379199825525
2	7.783237047577847	2.450681309913988
3	2.647635861260567	1.2504781136022052
4	1.3745387809610863	0.865592678126536
5	0.8769727881541657	0.6903235787677187
6	0.6524374569517276	0.616292272173695
7	0.4409630197764579	0.4859558285495044
8	0.36564751648721244	0.46052074693037454
9	0.2995591853493812	0.424445306647524
>10	2.614628098851583	8.657825147546129
>50	0.4308559784955558	4.764793563360818
>100	0.812659148564377	33.43672063287708
>500	0.2771015663651793	27.7345340646842
>1k	0.021227242143916428	4.726178520729868
>5k	5.737092471328764E-4	0.6202790362648442
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7455	0.3355253120205375	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6280	0.2826423822252147	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3278	0.14753212244176014	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3033	0.13650546899507582	No Hit
TGGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2727	0.12273340387391089	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2252	0.10135519821197188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027274089749757976	0.0
2	0.0	0.0	0.0	0.09064359200662139	0.0
3	0.0	0.0	0.0	0.12574885604096334	0.0
4	0.0	0.0	0.0	0.1816472380033386	0.0
5	0.0	0.0	0.0	0.3078011547831597	0.0
6	0.0	0.0	0.0	0.4513276765851039	0.0
7	0.0	0.0	0.0	0.5559233607079381	0.0
8	0.0	0.0	0.0	0.8850577144042749	0.0
9	0.0	0.0	0.0	1.0535179750203543	0.0
10	0.0	0.0	0.0	1.3416961873432922	0.0
11	0.0	0.0	0.0	1.5665949109068904	0.0
12	0.0	0.0	0.0	1.7615641465437741	0.0
13	0.0	0.0	0.0	1.8404609771235196	0.0
14	0.0	0.0	0.0	1.865034661947559	0.0
15	0.0	0.0	0.0	1.9036054456365732	0.0
16	0.0	0.0	0.0	1.9995148272483458	0.0
17	4.5006748761976855E-5	0.0	0.0	2.1105914831929047	0.0
18	4.5006748761976855E-5	0.0	0.0	2.301015037204829	0.0
19	4.5006748761976855E-5	0.0	0.0	2.3637544449790244	0.0
20	4.5006748761976855E-5	0.0	0.0	2.4337849460526604	0.0
21	4.5006748761976855E-5	0.0	0.0	2.5251486460394736	0.0
22	4.5006748761976855E-5	0.0	0.0	2.621508095138866	0.0
23	4.5006748761976855E-5	0.0	0.0	2.7350151155165716	0.0
24	4.5006748761976855E-5	0.0	0.0	2.820527938164328	0.0
25	4.5006748761976855E-5	0.0	0.0	2.8907834729817736	0.0
26	4.5006748761976855E-5	0.0	0.0	2.9587886703611206	0.0
27	4.5006748761976855E-5	0.0	0.0	3.0288641781835186	0.0
28	4.5006748761976855E-5	0.0	0.0	3.105330644330117	0.0
29	4.5006748761976855E-5	0.0	0.0	3.186477812347962	0.0
30	4.5006748761976855E-5	0.0	0.0	3.3093462364681585	0.0
31	4.5006748761976855E-5	0.0	0.0	3.4200178316738596	0.0
32	4.5006748761976855E-5	0.0	0.0	3.5149370648128686	0.0
33	4.5006748761976855E-5	0.0	0.0	3.614086932335504	0.0
34	4.5006748761976855E-5	0.0	0.0	3.7120216176415655	0.0
35	4.5006748761976855E-5	0.0	0.0	3.8576184498865604	0.0
36	4.5006748761976855E-5	0.0	0.0	3.9697752678014067	0.0
37	4.5006748761976855E-5	0.0	0.0	4.084002396159304	0.0
38	4.5006748761976855E-5	0.0	0.0	4.1905783772276655	0.0
39	4.5006748761976855E-5	0.0	0.0	4.293553818395068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	75	0.0	45.000004	12
TCACCAC	45	3.8562575E-10	45.000004	22
TCGGGCA	45	3.8562575E-10	45.000004	5
TGCGATA	75	0.0	45.000004	26
AGTGTCG	45	3.8562575E-10	45.000004	1
TGATCCG	20	7.0347113E-4	45.0	1
AACGTTG	85	0.0	45.0	1
CGGAACA	20	7.0347113E-4	45.0	20
TCGCACT	50	2.1827873E-11	45.0	23
CAAGTCG	35	1.2126111E-7	45.0	1
GGTCGAA	20	7.0347113E-4	45.0	8
CGAAAGT	55	1.8189894E-12	45.0	37
TTCACGG	20	7.0347113E-4	45.0	2
CGACAGA	20	7.0347113E-4	45.0	13
CGTTACA	35	1.2126111E-7	45.0	41
ATCCGGG	155	0.0	45.0	3
TAGCACG	55	1.8189894E-12	45.0	1
CCCCGTA	40	6.8193913E-9	45.0	34
TCAATCG	70	0.0	45.0	32
CCCGTAC	25	3.8919847E-5	45.0	15
>>END_MODULE