FastQCFastQC Report
Thu 26 May 2016
SRR1545121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545121_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1412448
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53150.3762970388998391No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG26710.1891043068488185No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16600.1175264505312762No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA16600.1175264505312762No Hit
TTTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA16490.11674766079884003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCG253.89126E-545.0000041
ACCGGGT453.8562575E-1045.0000046
CGAACAA253.89126E-545.00000435
TTGTGCG900.045.0000041
CGGCCTA453.8562575E-1045.0000042
CCGTATG502.1827873E-1145.0000041
ACGATGG1800.045.0000042
TACGCCC453.8562575E-1045.00000434
ACGTATG253.89126E-545.0000041
ACGTAAA453.8562575E-1045.00000422
TCGAGAG453.8562575E-1045.0000041
TTCGCGG502.1827873E-1145.0000042
GACTCGG253.89126E-545.0000042
TGCGTAG900.045.0000041
CGGGTAC406.8175723E-945.06
CTATGCG750.045.01
TTGGCGT351.2121927E-745.010
CGAACGG800.045.02
CGTATGA351.2121927E-745.015
ACGCATG351.2121927E-745.01