##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545121_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1412448
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.910930526291942	31.0	31.0	33.0	30.0	33.0
2	31.236024972246767	31.0	31.0	33.0	30.0	34.0
3	31.382046631097214	31.0	31.0	34.0	30.0	34.0
4	35.234085077822336	37.0	35.0	37.0	33.0	37.0
5	35.10416949862933	37.0	35.0	37.0	32.0	37.0
6	35.200542604046305	37.0	35.0	37.0	32.0	37.0
7	35.57135767122046	36.0	35.0	37.0	35.0	37.0
8	35.64422194657786	37.0	35.0	37.0	35.0	37.0
9	37.495704620630285	39.0	37.0	39.0	35.0	39.0
10	37.02211267246653	39.0	37.0	39.0	33.0	39.0
11	36.59463428034165	39.0	35.0	39.0	32.0	39.0
12	35.07150068533497	35.0	35.0	39.0	31.0	39.0
13	34.58721453816353	35.0	33.0	39.0	29.0	39.0
14	35.21035323070301	35.0	33.0	40.0	27.0	41.0
15	35.7129012891094	35.0	34.0	40.0	30.0	41.0
16	35.94192069371758	35.0	34.0	40.0	32.0	41.0
17	35.80357082172229	35.0	34.0	40.0	31.0	41.0
18	35.90626699177598	36.0	35.0	40.0	31.0	41.0
19	35.727730153605656	36.0	35.0	40.0	31.0	41.0
20	35.43092913863023	35.0	34.0	39.0	30.0	41.0
21	35.313267461881786	35.0	34.0	40.0	30.0	41.0
22	35.123092673146196	35.0	34.0	39.0	30.0	41.0
23	35.08110174675457	35.0	34.0	39.0	29.0	41.0
24	35.201639989578375	35.0	34.0	39.0	30.0	41.0
25	35.148225633793245	35.0	34.0	39.0	30.0	41.0
26	34.90412885996511	35.0	34.0	39.0	29.0	41.0
27	34.896786996760234	35.0	34.0	39.0	29.0	41.0
28	34.998228607354044	35.0	34.0	39.0	29.0	41.0
29	35.01486143206688	36.0	34.0	39.0	29.0	41.0
30	34.96918399827817	36.0	34.0	39.0	29.0	41.0
31	34.68348144498063	35.0	34.0	39.0	29.0	41.0
32	34.526866829787714	35.0	34.0	39.0	29.0	41.0
33	34.288880723396545	35.0	33.0	39.0	27.0	41.0
34	34.10698517750742	35.0	34.0	39.0	26.0	41.0
35	33.88042179251909	35.0	33.0	39.0	24.0	41.0
36	33.57556455175695	35.0	33.0	39.0	23.0	41.0
37	33.61690625070799	35.0	33.0	39.0	23.0	41.0
38	33.69106827295589	35.0	33.0	39.0	24.0	41.0
39	33.720196424930336	35.0	33.0	39.0	23.0	41.0
40	33.34408063164095	35.0	32.0	39.0	23.0	41.0
41	33.46382167697501	35.0	33.0	39.0	23.0	41.0
42	33.48018475724416	35.0	33.0	39.0	23.0	41.0
43	33.47035572283015	35.0	33.0	39.0	23.0	41.0
44	33.446964419221096	35.0	33.0	39.0	23.0	41.0
45	33.51434176691814	35.0	33.0	39.0	23.0	41.0
46	33.526624696979994	35.0	33.0	39.0	23.0	41.0
47	33.487903979473934	35.0	33.0	39.0	23.0	41.0
48	33.5392340107388	35.0	33.0	39.0	23.0	41.0
49	33.58990065474977	35.0	33.0	39.0	24.0	41.0
50	33.377453187657174	35.0	33.0	39.0	24.0	41.0
51	33.059589450372684	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	14.0
15	28.0
16	102.0
17	336.0
18	805.0
19	1651.0
20	3007.0
21	4818.0
22	7637.0
23	10931.0
24	15319.0
25	21217.0
26	26948.0
27	31211.0
28	33801.0
29	38240.0
30	45960.0
31	57576.0
32	72901.0
33	98400.0
34	186348.0
35	275254.0
36	72762.0
37	88205.0
38	128058.0
39	190800.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.284054351027436	25.78077210630055	29.84208976188858	13.093083780783434
2	30.547460862275994	26.551136749813093	30.963759373796417	11.937643014114501
3	27.629902127370354	26.05349011078638	34.281616031174245	12.034991730669024
4	26.297392895172074	28.54837841817893	31.96549536690908	13.188733319739912
5	21.385495253630577	33.54672172002084	32.39970604227554	12.668076984073043
6	18.950573755635606	42.565248419764835	28.921985092548542	9.56219273205102
7	87.09460454473368	4.714014250436122	6.889669566596433	1.3017116382337615
8	89.40152133034277	3.155160402365255	6.339065225764064	1.1042530415279006
9	85.7759011305195	4.567460182604952	8.068261628038696	1.5883770588368564
10	63.027240648859284	19.177626362174042	11.99251229071796	5.802620698248714
11	59.81551179229253	13.849996601644804	18.682953283943903	7.651538322118761
12	54.201499807426536	20.50220609891479	17.92349169668547	7.3728023969731975
13	17.433137361517026	56.46317598948776	17.260458438115954	8.843228210879268
14	8.380131516346088	55.155517229660845	25.29714368245769	11.167207571535377
15	7.471283898593081	22.97167754140329	59.325511452457015	10.231527107546615
16	7.938416139921611	15.326227939010852	58.34225401572306	18.39310190534448
17	8.57440415505562	16.253837309408915	31.643430413013434	43.52832812252203
18	15.729499422279616	22.057095199256892	45.50624164571014	16.707163732753347
19	33.678903577335234	18.836587258433585	28.1734973606108	19.31101180362038
20	33.8064834953216	19.399510636851762	30.06885917216067	16.725146695665966
21	15.547758218355648	35.98532476947824	30.65288067242121	17.814036339744895
22	18.981654545866466	24.7970190761005	20.106156120437706	36.115170257595324
23	11.119489000657016	35.48980210244908	25.44171537642448	27.94899352046943
24	8.95112598835497	20.34064262896758	59.04564274224609	11.662588640431364
25	8.69518736264981	25.147545254763358	50.08049853870726	16.076768843879563
26	8.230391490518588	42.952023720519264	29.782406148757335	19.03517864020481
27	8.167663517524185	53.56537019415936	26.068924307301934	12.198041981014523
28	6.974770044631732	36.5041403294139	44.42011316522803	12.100976460726342
29	8.1343171571626	25.21190160628922	42.33451426176397	24.319266974784203
30	13.222362876367837	45.55714617458484	28.373009130247627	12.8474818187997
31	33.3520242869118	30.99229139763021	25.443626951222274	10.21205736423571
32	37.01077844989691	20.96055925598677	30.259521058474366	11.769141235641948
33	37.73130055053354	27.027189673531343	19.109517660119167	16.131992115815947
34	19.295648406171413	25.552161920297245	24.31091268492716	30.841276988604182
35	19.383014454337435	23.149666394798253	36.70669645891388	20.760622691950427
36	39.522446136070144	27.28850902829697	22.170232107659892	11.018812727972994
37	15.165301660662905	46.58621060739935	27.58480312195564	10.663684609982102
38	14.969117447155577	45.3800069099889	22.8347521466277	16.816123496227824
39	20.055109993429845	41.257164865538414	25.93468927705657	12.753035863975171
40	29.076681053036996	23.64299429076327	24.298239652008427	22.982085004191305
41	12.050921520650672	23.52320227010127	28.786475679104644	35.63940053014341
42	15.16841681959265	19.901900813339676	25.984673418065658	38.945008949002016
43	15.946144566030041	20.476860033077322	29.976112394934184	33.60088300595845
44	10.494333242710528	31.30586046353565	35.87969256213326	22.320113731620562
45	9.732818482521125	56.076259090600146	20.329173180180792	13.861749246697933
46	17.33755862162713	44.60454473368223	23.283122635311177	14.77477400937946
47	16.350407236231	29.753307732390855	26.596660549627316	27.299624481750833
48	17.28460091982147	26.723532476947824	41.48973979927049	14.502126803960216
49	21.83216656471601	27.72109132513197	38.41925508054102	12.027487029611002
50	18.552399805160967	45.614281021319016	24.007609483676568	11.825709689843448
51	15.649779673304787	40.612822560547365	23.560300980991865	20.177096785155985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1284.0
1	1979.0
2	2674.0
3	6568.5
4	10463.0
5	8438.5
6	6414.0
7	6921.5
8	7429.0
9	7741.0
10	8053.0
11	7983.0
12	7913.0
13	7665.0
14	7417.0
15	6938.0
16	6459.0
17	6731.0
18	7003.0
19	6617.0
20	6231.0
21	5494.5
22	4758.0
23	6226.0
24	7694.0
25	8536.0
26	11498.0
27	13618.0
28	20650.0
29	27682.0
30	33000.5
31	38319.0
32	42320.0
33	46321.0
34	49954.5
35	53588.0
36	58341.5
37	63095.0
38	72898.0
39	82701.0
40	118279.5
41	153858.0
42	199083.0
43	244308.0
44	229929.5
45	215551.0
46	173172.5
47	130794.0
48	114059.0
49	97324.0
50	80295.5
51	63267.0
52	48124.5
53	32982.0
54	24630.5
55	16279.0
56	12930.5
57	9582.0
58	8562.0
59	7542.0
60	6976.5
61	6411.0
62	5719.0
63	5027.0
64	4295.5
65	3564.0
66	3027.0
67	2490.0
68	2165.0
69	1840.0
70	1495.0
71	1150.0
72	964.0
73	778.0
74	645.5
75	426.0
76	339.0
77	268.5
78	198.0
79	141.0
80	84.0
81	58.5
82	33.0
83	23.5
84	14.0
85	12.5
86	11.0
87	8.5
88	6.0
89	6.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1412448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.708962688924563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.99252041226346	12.723084812382076
2	7.435593060996096	2.336109079304281
3	2.5962666404404704	1.223539673555366
4	1.3166026865392837	0.8272984991593381
5	0.8976313764650304	0.705042890064858
6	0.6500767311364759	0.6127218668616566
7	0.5077332509241601	0.5583173886285803
8	0.4011564590218293	0.504140147775601
9	0.3208431808998698	0.45361022019767366
>10	2.9748462563406965	9.762542707337376
>50	0.4918260265850517	5.569805386466681
>100	1.324946768304813	54.431914640444965
>500	0.08408055233868825	8.715909266625234
>1k	0.0054245517637863385	1.1985360529424283
>5k	4.5204598031552823E-4	0.3774273682538811
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5315	0.3762970388998391	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2671	0.1891043068488185	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1660	0.1175264505312762	No Hit
AGTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1660	0.1175264505312762	No Hit
TTTATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1649	0.11674766079884003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016850177847255263	0.0
2	0.0	0.0	0.0	0.06237397766147851	0.0
3	0.0	0.0	0.0	0.09310077255941458	0.0
4	0.0	0.0	0.0	0.1430141145019144	0.0
5	0.0	0.0	0.0	0.2502039013117651	0.0
6	0.0	0.0	0.0	0.35498651985772217	0.0
7	0.0	0.0	0.0	0.4255023901764879	0.0
8	0.0	0.0	0.0	0.6399527628627745	0.0
9	0.0	0.0	0.0	0.7259736287636783	0.0
10	0.0	0.0	0.0	0.8666513740682843	0.0
11	0.0	0.0	0.0	0.9850274133985818	0.0
12	0.0	0.0	0.0	1.091226013276241	0.0
13	0.0	0.0	0.0	1.1316522802963365	0.0
14	0.0	0.0	0.0	1.1464492852126238	0.0
15	0.0	0.0	0.0	1.1707333650513152	0.0
16	0.0	0.0	0.0	1.2323285529803576	0.0
17	0.0	0.0	0.0	1.3075171616937402	0.0
18	0.0	0.0	0.0	1.436796257278144	0.0
19	0.0	0.0	0.0	1.4856476132218672	0.0
20	0.0	0.0	0.0	1.5391717075602076	0.0
21	0.0	0.0	0.0	1.6090503862797072	0.0
22	0.0	0.0	0.0	1.6861505697908878	0.0
23	0.0	0.0	0.0	1.7771981694193344	0.0
24	0.0	0.0	0.0	1.8437492920093341	0.0
25	0.0	0.0	0.0	1.8975565826140148	0.0
26	0.0	0.0	0.0	1.9563198078796529	0.0
27	0.0	0.0	0.0	2.0133838555472483	0.0
28	0.0	0.0	0.0	2.0748374453431206	0.0
29	0.0	0.0	0.0	2.1440789324633545	0.0
30	0.0	0.0	0.0	2.24772876594395	0.0
31	0.0	0.0	0.0	2.341749926368971	0.0
32	0.0	0.0	0.0	2.4213280772106303	0.0
33	0.0	0.0	0.0	2.502605405650332	0.0
34	0.0	0.0	0.0	2.5949273884773105	0.0
35	0.0	0.0	0.0	2.728100432723895	0.0
36	0.0	0.0	0.0	2.8236791726137884	0.0
37	0.0	0.0	0.0	2.9232226602324474	0.0
38	0.0	0.0	0.0	3.01823500758966	0.0
39	0.0	0.0	0.0	3.119760869072702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTCG	25	3.89126E-5	45.000004	1
ACCGGGT	45	3.8562575E-10	45.000004	6
CGAACAA	25	3.89126E-5	45.000004	35
TTGTGCG	90	0.0	45.000004	1
CGGCCTA	45	3.8562575E-10	45.000004	2
CCGTATG	50	2.1827873E-11	45.000004	1
ACGATGG	180	0.0	45.000004	2
TACGCCC	45	3.8562575E-10	45.000004	34
ACGTATG	25	3.89126E-5	45.000004	1
ACGTAAA	45	3.8562575E-10	45.000004	22
TCGAGAG	45	3.8562575E-10	45.000004	1
TTCGCGG	50	2.1827873E-11	45.000004	2
GACTCGG	25	3.89126E-5	45.000004	2
TGCGTAG	90	0.0	45.000004	1
CGGGTAC	40	6.8175723E-9	45.0	6
CTATGCG	75	0.0	45.0	1
TTGGCGT	35	1.2121927E-7	45.0	10
CGAACGG	80	0.0	45.0	2
CGTATGA	35	1.2121927E-7	45.0	15
ACGCATG	35	1.2121927E-7	45.0	1
>>END_MODULE