FastQCFastQC Report
Thu 26 May 2016
SRR1545120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545120_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1665845
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71630.4299919860491222No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18770.11267554904567953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16970.1018702220194556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA302.1660908E-645.0000046
GATCGGA600.045.0000049
CGTTGTG302.1660908E-645.00000415
GACCGTA302.1660908E-645.00000417
CACGACG650.045.00000426
TCTACCG650.045.00000412
GTACGCC302.1660908E-645.00000416
TCACGAA207.0342014E-445.037
CTATGCG750.045.01
ACTATCG406.8175723E-945.01
ACACGCG502.1827873E-1145.01
GGTCGAA253.891563E-545.011
TAAACGG1100.045.02
ATTCGTC207.0342014E-445.014
GATTTCG406.8175723E-945.013
GCGTTAA207.0342014E-445.012
ACGGGTA351.2123746E-745.05
TCTAGCG351.2123746E-745.01
CGTTAGG2450.045.02
ATCCGCC207.0342014E-445.018
Produced by FastQC (version 0.11.5)