##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545120_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1665845
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.849842572388187	31.0	31.0	33.0	30.0	33.0
2	31.165615648514716	31.0	31.0	33.0	30.0	34.0
3	31.30623557413805	31.0	31.0	34.0	28.0	34.0
4	35.16172392989744	35.0	35.0	37.0	32.0	37.0
5	35.04861916925044	37.0	35.0	37.0	32.0	37.0
6	35.11955794206544	37.0	35.0	37.0	32.0	37.0
7	35.5240301468624	36.0	35.0	37.0	35.0	37.0
8	35.59821772133662	37.0	35.0	37.0	35.0	37.0
9	37.43395213840423	39.0	37.0	39.0	35.0	39.0
10	36.93825596018837	39.0	37.0	39.0	32.0	39.0
11	36.49661883308471	38.0	35.0	39.0	32.0	39.0
12	35.03816321446473	35.0	34.0	39.0	30.0	39.0
13	34.53688008188037	35.0	33.0	39.0	27.0	39.0
14	35.163405358841906	35.0	33.0	40.0	27.0	41.0
15	35.65603342447827	35.0	34.0	40.0	30.0	41.0
16	35.89007200549871	35.0	34.0	40.0	31.0	41.0
17	35.74354876954339	35.0	34.0	40.0	31.0	41.0
18	35.83205040084762	36.0	35.0	40.0	31.0	41.0
19	35.69786684835624	36.0	34.0	40.0	31.0	41.0
20	35.38673586077937	35.0	34.0	39.0	30.0	41.0
21	35.22783212123577	35.0	34.0	39.0	30.0	41.0
22	35.047797964396445	35.0	34.0	39.0	29.0	41.0
23	34.97898784100562	35.0	34.0	39.0	29.0	41.0
24	35.084350584838326	35.0	34.0	39.0	30.0	41.0
25	35.00304890310923	35.0	34.0	39.0	30.0	41.0
26	34.768315779679384	35.0	34.0	39.0	29.0	41.0
27	34.744991280701385	35.0	34.0	39.0	29.0	41.0
28	34.86398734576146	35.0	34.0	39.0	29.0	41.0
29	34.86099547076709	36.0	34.0	39.0	29.0	41.0
30	34.83306730217998	36.0	34.0	39.0	29.0	41.0
31	34.54035099303957	35.0	34.0	39.0	29.0	41.0
32	34.345619790556746	35.0	34.0	39.0	27.0	41.0
33	34.078511506172546	35.0	33.0	39.0	26.0	41.0
34	33.859398083255044	35.0	33.0	39.0	24.0	41.0
35	33.639142297152496	35.0	33.0	39.0	23.0	41.0
36	33.3530670620616	35.0	33.0	39.0	23.0	41.0
37	33.387537856163085	35.0	33.0	39.0	23.0	41.0
38	33.4417487821496	35.0	33.0	39.0	23.0	41.0
39	33.43853719883903	35.0	33.0	39.0	23.0	41.0
40	33.07717284621318	35.0	32.0	39.0	23.0	41.0
41	33.21070987997083	35.0	32.0	39.0	23.0	41.0
42	33.19944112447437	35.0	32.0	39.0	23.0	41.0
43	33.16001608793135	35.0	32.0	39.0	23.0	41.0
44	33.124629842512356	35.0	32.0	39.0	23.0	41.0
45	33.228781789422186	35.0	33.0	39.0	23.0	41.0
46	33.22468657047925	35.0	33.0	38.0	23.0	41.0
47	33.167151205544336	35.0	32.0	38.0	23.0	41.0
48	33.229836509399135	35.0	33.0	38.0	23.0	41.0
49	33.28891283402717	35.0	33.0	38.0	23.0	41.0
50	33.05054972101246	35.0	32.0	38.0	23.0	40.0
51	32.75668204424782	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	15.0
15	55.0
16	152.0
17	449.0
18	1097.0
19	2311.0
20	3963.0
21	6667.0
22	10108.0
23	14289.0
24	20160.0
25	27351.0
26	34944.0
27	39323.0
28	42624.0
29	47518.0
30	56448.0
31	69242.0
32	87964.0
33	117201.0
34	214694.0
35	311161.0
36	90571.0
37	106859.0
38	151685.0
39	208894.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.308342012612215	24.541418919527327	31.305193460375964	13.84504560748449
2	29.741722669275955	26.593110403428888	31.40376205469296	12.261404872602192
3	27.165612647034987	26.01124354306673	34.579207549321815	12.243936260576465
4	24.825719079506197	28.890503017987868	33.229922351719395	13.053855550786539
5	22.570527269944083	32.24111486963073	32.897958693635964	12.290399166789227
6	19.375332038695074	41.87496435742821	29.105228877836776	9.644474726039938
7	86.32940039439444	4.984977593953819	7.512883851738908	1.172738159912837
8	88.88684121271787	3.016126950586639	7.1112858639309176	0.9857459727645729
9	84.88887021301503	4.600187892631067	8.699488848002066	1.8114530463518514
10	61.1042443924855	20.260048203764455	12.484955082855848	6.150752320894201
11	58.65419651888381	14.211046045700531	19.87789980460367	7.25685763081199
12	52.55362893906697	20.39229340064652	19.57427011516678	7.479807545119745
13	17.724938394628552	53.42675939238044	19.15568375208978	9.692618460901224
14	9.390009274572364	53.91840177207363	25.84424121091698	10.847347742437021
15	8.121283792909905	23.307030365970423	59.209050061680415	9.362635779439264
16	8.572106048281803	16.197425330688027	57.89746344948059	17.333005171549573
17	9.481914583889859	16.20168743190393	32.014503150052974	42.30189483415324
18	14.766559914037622	22.895167317487523	45.429316653109986	16.908956115364877
19	33.85543072734858	18.809853257656027	28.85418511326084	18.480530901734554
20	33.96474461909722	19.292131020593153	30.024161911822528	16.718962448487105
21	16.502495730395083	35.13622215752366	31.457968778607853	16.903313333473402
22	19.406607457476536	24.434146033994757	20.71321161332537	35.44603489520334
23	13.177636574831391	35.748403963153834	24.8506313612611	26.223328100753672
24	9.869045439401624	20.213105060794973	57.43493542316362	12.482914076639783
25	9.016805285005509	25.54511374107435	50.23804735734717	15.20003361657297
26	8.698108167326492	42.79017555654938	30.91650183540485	17.595214440719275
27	8.89644594785229	51.756015715747864	26.483796511680257	12.863741824719588
28	8.068157601697637	34.841356788896924	44.373276025080365	12.717209584325072
29	8.556018116931648	24.34380149413661	43.95282874457107	23.14735164436067
30	12.517190975150749	44.24259159765764	29.985742971284846	13.254474455906761
31	32.74638396729588	31.13987195687474	25.723701784979998	10.390042290849388
32	36.69915268227236	22.710516284528275	28.945790274605383	11.644540758593987
33	37.274836494391735	26.868045946651698	20.338206735920807	15.518910823035755
34	18.653476163748728	26.285638819938228	24.557987087634203	30.50289792867884
35	18.641950481587422	24.3225510176517	36.59115944160471	20.444339059156164
36	38.57255627024123	26.201357269133684	22.633918521831262	12.592167938793825
37	16.086790787858416	45.57806998850433	27.60767058159673	10.727468642040526
38	16.021538618538937	44.00151274578367	23.808937806338527	16.168010829338865
39	19.4500088543652	40.82054452845253	26.444957363980443	13.284489253201828
40	28.537228853824935	24.613394403440896	25.593317505530226	21.256059237203942
41	13.364808850763426	23.86056325768604	27.628800998892455	35.145826892658086
42	15.837067674363462	20.509110991718916	25.79267578916406	37.86114554475357
43	15.979758020704207	21.863378645672316	29.13950577634774	33.01735755727574
44	10.859293631760458	31.115920148633275	36.20072695839049	21.82405926121578
45	10.59852507286092	53.74959855208618	21.227004913422316	14.424871461630584
46	17.496225639240144	45.006288100033316	22.9725454649142	14.524940795812336
47	17.614543970177298	29.83398815616099	25.226656741773695	27.324811131888023
48	17.415785982489368	25.995215641311166	41.51070477745529	15.07829359874418
49	21.40769399313862	28.135450777233178	38.293178537018754	12.163676692609457
50	17.901125254750593	44.83190212774898	24.761007176538033	12.505965440962393
51	15.907362329628508	40.43689538942699	24.590523127902056	19.065219153042452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2222.0
1	2749.5
2	3277.0
3	8439.0
4	13601.0
5	10995.0
6	8389.0
7	8995.5
8	9602.0
9	10161.5
10	10721.0
11	10587.5
12	10454.0
13	10452.0
14	10450.0
15	9452.0
16	8454.0
17	8169.5
18	7885.0
19	8456.0
20	9027.0
21	7681.5
22	6336.0
23	7498.0
24	8660.0
25	9945.5
26	13544.0
27	15857.0
28	20988.0
29	26119.0
30	33500.0
31	40881.0
32	44702.5
33	48524.0
34	63069.5
35	77615.0
36	75997.0
37	74379.0
38	92952.5
39	111526.0
40	147047.0
41	182568.0
42	224363.0
43	266158.0
44	253724.5
45	241291.0
46	206461.0
47	171631.0
48	141669.5
49	111708.0
50	92054.0
51	72400.0
52	55081.5
53	37763.0
54	28265.5
55	18768.0
56	15409.0
57	12050.0
58	10574.5
59	9099.0
60	8354.5
61	7610.0
62	6790.0
63	5970.0
64	4987.0
65	4004.0
66	3467.5
67	2931.0
68	2605.0
69	2279.0
70	2005.5
71	1732.0
72	1451.0
73	1170.0
74	942.5
75	546.5
76	378.0
77	276.5
78	175.0
79	153.0
80	131.0
81	100.0
82	69.0
83	45.0
84	21.0
85	14.5
86	8.0
87	5.0
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1665845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.615899320255245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4250363429236	13.529502060221429
2	7.251051307853482	2.409654769945971
3	2.596905891066321	1.2944978049040716
4	1.3857432064691166	0.9210147840967406
5	0.8908321792834376	0.7400988901108584
6	0.6483136317722427	0.6463388420085968
7	0.5019628606598282	0.5838395048661712
8	0.3702497366060656	0.4921625877437927
9	0.30687168880274035	0.45890541768447296
>10	2.921682891543314	10.192357573094766
>50	0.45381432544923955	5.382891853607788
>100	1.115128081445786	48.50148047367088
>500	0.12225054113442742	12.380458336693078
>1k	0.009794553740740477	2.035039798065355
>5k	3.627612496570547E-4	0.4317573032860061
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7163	0.4299919860491222	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1877	0.11267554904567953	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1697	0.1018702220194556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018729233512121475	0.0
2	0.0	0.0	0.0	0.07419657891340431	0.0
3	0.0	0.0	0.0	0.10721285593797743	0.0
4	0.0	0.0	0.0	0.1658617698525373	0.0
5	0.0	0.0	0.0	0.29318453997820926	0.0
6	0.0	0.0	0.0	0.42110760604978253	0.0
7	0.0	0.0	0.0	0.5106717611782609	0.0
8	0.0	0.0	0.0	0.7544519448087907	0.0
9	0.0	0.0	0.0	0.844916543856121	0.0
10	0.0	0.0	0.0	0.9908484883047343	0.0
11	0.0	0.0	0.0	1.1143293643766377	0.0
12	0.0	0.0	0.0	1.2249038776116625	0.0
13	0.0	0.0	0.0	1.2703462807163932	0.0
14	0.0	0.0	0.0	1.2852936497693364	0.0
15	0.0	0.0	0.0	1.3109863162539133	0.0
16	0.0	0.0	0.0	1.3713760884115869	0.0
17	0.0	0.0	0.0	1.4434116019197465	0.0
18	0.0	0.0	0.0	1.567372714748371	0.0
19	0.0	0.0	0.0	1.6195384324471964	0.0
20	0.0	0.0	0.0	1.6727846828486443	0.0
21	0.0	0.0	0.0	1.7542448427074548	0.0
22	0.0	0.0	0.0	1.8318030789179065	0.0
23	0.0	0.0	0.0	1.9301315548565443	0.0
24	0.0	0.0	0.0	2.004388163364539	0.0
25	0.0	0.0	0.0	2.067659356062539	0.0
26	0.0	0.0	0.0	2.1349525315980777	0.0
27	0.0	0.0	0.0	2.198283753890668	0.0
28	0.0	0.0	0.0	2.265997136588338	0.0
29	0.0	0.0	0.0	2.34367543198797	0.0
30	0.0	0.0	0.0	2.458812194411845	0.0
31	0.0	0.0	0.0	2.5673457014308054	0.0
32	0.0	0.0	0.0	2.6572700341268245	0.0
33	0.0	0.0	0.0	2.7450333014175987	0.0
34	0.0	0.0	0.0	2.845462813166891	0.0
35	0.0	0.0	0.0	2.9861121532915726	0.0
36	0.0	0.0	0.0	3.096386518553647	0.0
37	0.0	0.0	0.0	3.2109229850316208	0.0
38	0.0	0.0	0.0	3.312853236645666	0.0
39	0.0	0.0	0.0	3.4141831923138106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	30	2.1660908E-6	45.000004	6
GATCGGA	60	0.0	45.000004	9
CGTTGTG	30	2.1660908E-6	45.000004	15
GACCGTA	30	2.1660908E-6	45.000004	17
CACGACG	65	0.0	45.000004	26
TCTACCG	65	0.0	45.000004	12
GTACGCC	30	2.1660908E-6	45.000004	16
TCACGAA	20	7.0342014E-4	45.0	37
CTATGCG	75	0.0	45.0	1
ACTATCG	40	6.8175723E-9	45.0	1
ACACGCG	50	2.1827873E-11	45.0	1
GGTCGAA	25	3.891563E-5	45.0	11
TAAACGG	110	0.0	45.0	2
ATTCGTC	20	7.0342014E-4	45.0	14
GATTTCG	40	6.8175723E-9	45.0	13
GCGTTAA	20	7.0342014E-4	45.0	12
ACGGGTA	35	1.2123746E-7	45.0	5
TCTAGCG	35	1.2123746E-7	45.0	1
CGTTAGG	245	0.0	45.0	2
ATCCGCC	20	7.0342014E-4	45.0	18
>>END_MODULE