FastQCFastQC Report
Thu 26 May 2016
SRR1545118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545118_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1737728
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73640.4237717295226871No Hit
TTTGACGGGCAGGACACTTATTAATACACCTAATCGGAGGAGCTACTCTAG27910.16061201753093693No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24800.14271508544490277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21750.12516343179139658No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18930.10893534546258102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC302.1661454E-645.00000416
CGGGTAC302.1661454E-645.0000046
AGCGTCG351.212411E-745.00000417
TAATACG207.034284E-445.0000041
CGAACGG207.034284E-445.0000042
GCGACTA207.034284E-445.00000421
GTCGATG600.045.0000041
GTCGATA406.8193913E-945.0000049
GGTCGCG207.034284E-445.00000428
CACGGAT351.212411E-745.00000419
CGAAACA207.034284E-445.00000417
ACGTTAG406.8193913E-945.0000041
ACGTTAC207.034284E-445.0000041
TCTTCGA650.045.00000427
TAGCGAA650.045.0000041
GACCGAC850.045.00000431
ACCGACA850.045.00000432
CCGTGAG406.8193913E-945.0000042
GCACGGA207.034284E-445.0000043
CCTACCG207.034284E-445.00000437